{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T12:08:54Z","timestamp":1779797334631,"version":"3.53.1"},"reference-count":10,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"<jats:p>\n                    Tuberculous lymphadenitis, a form of extra-pulmonary tuberculosis, is diagnosed through the detection of\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    in tissue samples. Lymphadenitis may be caused by both nontuberculous mycobacteria (NTMs) and\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    complex (MTBC). Here, we highlight the advantage of using a fluorescent dye in combination with Confocal Laser Scanning Microscopy (CLSM) for identifying\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    in histological sections of lymph node tissue after correlating with Clinical, histopathological, and microbiological findings. CLSM enables the differentiation of non-specific fluorescence from the\n                    <jats:italic>Mycobacterium tuberculosis<\/jats:italic>\n                    fluorescence through the spectral emission method. This method proved effective even in lymph node tissue samples exhibiting weak staining using the Ziehl-Neelsen method. Overall, the integration of fluorescent dye with CLSM visualisation substantially improves bacillary visualisation, facilitates faster processing, and reduces observer fatigue due to the black background.\n                  <\/jats:p>","DOI":"10.25259\/ijmr_3219_2025","type":"journal-article","created":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T11:06:55Z","timestamp":1777979215000},"page":"606-609","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Application of confocal microscopy for detecting tuberculous lymphadenitis"],"prefix":"10.25259","volume":"163","author":[{"given":"Bhagyashri","family":"Patil-Takbhate","sequence":"first","affiliation":[{"name":"Central Research Facility, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charusheela","family":"Gore","sequence":"additional","affiliation":[{"name":"Department of Pathology, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Srikanth","family":"Tripathy","sequence":"additional","affiliation":[{"name":"Central Research Facility, Dr. D. Y. Patil Medical College, Hospital and Research Centre, Dr. D. Y. Patil Vidyapeeth, Pune, Maharashtra, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"10894","published-online":{"date-parts":[[2026,5,4]]},"reference":[{"key":"10.25259\/IJMR_3219_2025\/ref-1","doi-asserted-by":"crossref","first-page":"1185","DOI":"10.3390\/jcm8081185","article-title":"Application of laser scanning confocal microscopy for the visualization of m. tuberculosis in lung tissue samples with weak Ziehl\u2013Neelsen staining","volume":"8","author":"Erokhina","year":"2019","journal-title":"JCM."},{"key":"10.25259\/IJMR_3219_2025\/ref-2","doi-asserted-by":"publisher","first-page":"100116","DOI":"10.1016\/j.medmic.2024.100116","article-title":"Diagnosis of tuberculous lymphadenitis by molecular and immunological tools","volume":"22","author":"Kumar","year":"2024","journal-title":"Med Microecology."},{"key":"10.25259\/IJMR_3219_2025\/ref-3","doi-asserted-by":"publisher","first-page":"14","DOI":"10.1155\/2013\/824620","article-title":"Correlation of various techniques in diagnosis of tuberculous lymphadenitis on fine needle aspiration cytology","volume":"2013","author":"Thakur","year":"2013","journal-title":"Pathol Res Int."},{"key":"10.25259\/IJMR_3219_2025\/ref-4","first-page":"22630","article-title":"A comparative study of fluorescent microscopy with Ziehl-Neelsen staining and culture for the diagnosis of pulmonary tuberculosis","volume":"7","author":"Laifangbam","year":"2010","journal-title":"Kathmandu Univ Med J."},{"key":"10.25259\/IJMR_3219_2025\/ref-5","first-page":"577","article-title":"Comparison of Ziehl\u2013Neelsen-based light microscopy with LED fluorescent microscopy for tuberculosis diagnosis: an insight from a limited resource\u2013high burden setting","volume":"29","author":"Noori","year":"2017","journal-title":"J Ayub Med Coll Abbottabad."},{"key":"10.25259\/IJMR_3219_2025\/ref-6","doi-asserted-by":"publisher","first-page":"57081","DOI":"10.1016\/s1473-3099(06)70578-3","article-title":"Fluorescence versus conventional sputum smear microscopy for tuberculosis: A systematic review","volume":"6","author":"Steingart","year":"2006","journal-title":"Lancet Infect Dis."},{"key":"10.25259\/IJMR_3219_2025\/ref-7","doi-asserted-by":"publisher","first-page":"e0205233","DOI":"10.1371\/journal.pone.0205233","article-title":"Cost-effectiveness of GeneXpert and LED-FM for diagnosis of pulmonary tuberculosis: A systematic review","volume":"13","author":"Sagili","year":"2018","journal-title":"PLoS One."},{"key":"10.25259\/IJMR_3219_2025\/ref-8","doi-asserted-by":"crossref","first-page":"763","DOI":"10.51253\/pafmj.v72i3.4323","article-title":"Comparison of LED fluorescent microscopy with Ziehl\u2013Neelsen microscopy on sputum specimens for diagnosis of pulmonary tuberculosis, keeping culture as the gold standard","volume":"72","author":"Zahra","year":"2022","journal-title":"Pak Armed Forces Med J."},{"key":"10.25259\/IJMR_3219_2025\/ref-9","first-page":"363","article-title":"Comparison of conventional Ziehl\u2013Neelsen staining with LED fluorescent staining for pulmonary tuberculosis","volume":"3","author":"Khanna","year":"2016","journal-title":"Indian J Microbiol Res."},{"key":"10.25259\/IJMR_3219_2025\/ref-10","first-page":"383","article-title":"A comparative study of Ziehl\u2013Neelsen staining and auramine staining in sputum samples for the diagnosis of pulmonary tuberculosis","volume":"5","author":"Bhumbla","year":"2014","journal-title":"Int J Biomed Res."}],"container-title":["The Indian Journal of Medical Research"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/ijmr.org.in\/application-of-confocal-microscopy-for-detecting-tuberculous-lymphadenitis\/","content-type":"text\/html","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/ijmr.org.in\/application-of-confocal-microscopy-for-detecting-tuberculous-lymphadenitis\/","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T11:54:21Z","timestamp":1779796461000},"score":1,"resource":{"primary":{"URL":"http:\/\/ijmr.org.in\/application-of-confocal-microscopy-for-detecting-tuberculous-lymphadenitis\/"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5,4]]},"references-count":10,"alternative-id":["10.25259\/IJMR_3219_2025"],"URL":"https:\/\/doi.org\/10.25259\/ijmr_3219_2025","relation":{},"ISSN":["0971-5916"],"issn-type":[{"value":"0971-5916","type":"print"}],"subject":[],"published":{"date-parts":[[2026,5,4]]},"article-number":"606"}}