{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,13]],"date-time":"2026-10-13T05:14:03Z","timestamp":1791868443444,"version":"4.3.4"},"reference-count":25,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"\n Background & objectives<\/jats:title>\n \n Brucellosis, an occupational zoonosis, poses notable difficulties in diagnosis because of the need for secure facilities and the risk of exposure when using culture-based methods. Conventional serological tests often suffer from cross-reactivity, highlighting the need for more specific diagnostic markers. The purpose of this study was to understand the differences in whole-cell protein expression of\n Brucella melitensis<\/jats:italic>\n isolates from patients with acute and chronic brucellosis and to identify immunodominant proteins that could serve as potential diagnostic markers.\n <\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n \n The study included 16 blood culture-confirmed patients with acute or chronic brucellosis from a cohort of 185 individuals. Clinical isolates of\n B. melitensis<\/jats:italic>\n were subjected to two-dimensional gel electrophoresis (2-DE) and PDQuest software analysis to compare proteomic profiles. Differentially expressed proteins were identified using western blotting with infected human serum samples. Further identification of the protein spots was conducted using Matrix-Assisted Laser Desorption\/Ionization - Time-of-flight Mass Spectrometry (MALDI-TOF MS).\n <\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n \n The proteomic analysis of\n B. melitensis<\/jats:italic>\n identified an average of 249 protein spots. PDQuest software revealed 65 differentially expressed spots, with 17 of these spots successfully identified\n via<\/jats:italic>\n MALDI-TOF MS. Western blot assays demonstrated that human sera containing anti-Brucella antibodies reacted with seven immunodominant protein spots. Among these, the outer membrane porin protein (omp25), aldehyde dehydrogenase, and universal stress protein showed potential for differentiating acute from chronic brucellosis.\n <\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions<\/jats:title>\n \n The findings indicate that omp25, aldehyde dehydrogenase, and universal stress protein are highly specific to\n B. melitensis<\/jats:italic>\n infections and could serve as valuable targets for serodiagnosis.\n <\/jats:p>\n <\/jats:sec>","DOI":"10.25259\/ijmr_325_2025","type":"journal-article","created":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T14:40:52Z","timestamp":1767278452000},"page":"644-651","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":1,"title":["Comparative analysis of\n Brucella melitensis<\/i>\n whole cell proteomes for diagnosis of human brucellosis"],"prefix":"10.25259","volume":"162","author":[{"given":"Sudipta","family":"Patra","sequence":"first","affiliation":[{"name":"Department of Microbiology, Sapthagiri Institute of Medical Sciences & Research Centre, Bengaluru, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chaitanya","family":"Tellapragada","sequence":"additional","affiliation":[{"name":"Department of Clinical Microbiology, Karolisnka Institute, Stockholm, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Prachi","family":"Pathak","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Defence Research & Development Establishment, Gwalior, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M. Kameswara","family":"Rao","sequence":"additional","affiliation":[{"name":"Department of Pharmacology & Toxicology, Defence Research & Development Establishment, Gwalior, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vandana Eshwara","family":"Kalwaje","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Kasturba Medical College, Manipal, Karnataka, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Duraipandian","family":"Thavaselvam","sequence":"additional","affiliation":[{"name":"Department of Biodetector Development Test & Evaluation, Defence Research & Development Establishment, Gwalior, Madhya Pradesh, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chiranjay","family":"Mukhopadhyay","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Kasturba Medical College, Manipal, Karnataka, 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