{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T17:44:08Z","timestamp":1765215848959,"version":"3.46.0"},"reference-count":68,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"\n Background & objectives<\/jats:title>\n The impact of cell passaging and cryopreservation on the phenotypic and functional attributes of adipose-derived stem cells (ADSCs) must be understood to improve their clinical value. This systematic review investigates the phenotypic characteristics of ADSCs derived from fresh and cryopreserved adipose tissue, with a focus on how these cells change across passages.<\/jats:p>\n <\/jats:sec>\n \n Methods<\/jats:title>\n A thorough search of databases was conducted as per the PRISMA guideline to find publications that were aligned with the inclusion criteria and were published between January 2013 and January 2023.<\/jats:p>\n <\/jats:sec>\n \n Results<\/jats:title>\n In both cryopreserved and fresh stromal vascular fraction (SVF) cells, CD90, CD73, and CD105 consistently exhibited strong expression (90%) across passages in 50 screened studies. In fresh tissue, CD29 was upregulated in subsequent passages (up to 95%) but downregulated at passage 2 (2.3%). Variable CD29 was seen in cryopreserved groups (47% at P1, 90% at P4). While CD34 and CD45 were lower in cryopreserved ADSCs (less than 5%), they were higher in fresh tissue (41%), suggesting less haematopoietic contamination.<\/jats:p>\n <\/jats:sec>\n \n Interpretation & conclusions<\/jats:title>\n Passaging and cryopreservation protocols can help maintain the therapeutic potential of ADSCs, providing a reliable source of functional stem cells for regenerative utilization.<\/jats:p>\n <\/jats:sec>","DOI":"10.25259\/ijmr_686_2025","type":"journal-article","created":{"date-parts":[[2025,12,8]],"date-time":"2025-12-08T17:40:25Z","timestamp":1765215625000},"page":"479-492","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Unveiling the phenotypic impact of cryopreservation on adipose derived stem cells: A systematic review"],"prefix":"10.25259","volume":"162","author":[{"given":"Sadia","family":"Farhana","sequence":"first","affiliation":[{"name":"Department of Plastic and Reconstructive 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