{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T18:40:14Z","timestamp":1751308814808,"version":"3.41.0"},"reference-count":29,"publisher":"Scientific Scholar","content-domain":{"domain":["ijmr.org.in"],"crossmark-restriction":true},"short-container-title":["Indian J Med Res"],"abstract":"\nBackground & objectives<\/jats:title>\nThe role of human herpesvirus-8 (HHV-8) and Leishmania<\/jats:italic> species in the aetiology of malignant skin tumours and proliferative skin diseases remains a topic of debate. This study aims to analyse formalin-fixed, paraffin-embedded (FFPE) skin biopsy samples using polymerase chain reaction (PCR) to determine whether skin lesions caused by HHV-8 and Leishmania<\/jats:italic> spp. resemble malignant and proliferative skin diseases and assess the role of these pathogens in disease aetiology.<\/jats:p>\n<\/jats:sec>\n\nMethods<\/jats:title>\nIn this retrospective, single-center observational study, skin biopsies were collected from 275 individuals diagnosed with malignant skin tumours, psoriasis, actinic keratoses, seborrheic keratoses, and chronic dermatitis. The presence of HHV-8 and Leishmania<\/jats:italic> spp. in biopsy samples was evaluated using PCR.<\/jats:p>\n<\/jats:sec>\n\nResults<\/jats:title>\nHHV-8 DNA was not detected in any of the samples using PCR. However, Leishmania<\/jats:italic> spp. DNA was identified in 8.4 per cent of all samples (n=23). No positivity was observed in the control group (P<\/jats:italic>=0.387). Leishmania<\/jats:italic> spp. DNA PCR positivity was most frequently detected in psoriasis cases (32.4%), followed by actinic keratosis (AK) (8.7%), malignant skin tumours (4.2%), and seborrheic keratosis (SK) (3.8%). When the Leishmania<\/jats:italic> positivity rate in individuals diagnosed with psoriasis was compared with that of the control group, the difference was found to be significant (P<\/jats:italic>=0.002). The positivity rate in squamous cell carcinoma (SCC) (7.3%) was higher than in basal cell carcinoma (1.6%).<\/jats:p>\n<\/jats:sec>\n\nInterpretation & conclusions<\/jats:title>\nThe findings in this study suggests that there is no relationship between malignant and proliferative skin diseases and HHV-8. However, Leishmania<\/jats:italic> spp. DNA was detected in 8.4 per cent of all samples. Biopsy-archived samples may be preferred for the differential diagnosis of Leishmania<\/jats:italic> in diseases that do not respond to treatment and in atypical clinical presentations.<\/jats:p>\n<\/jats:sec>","DOI":"10.25259\/ijmr_849_2024","type":"journal-article","created":{"date-parts":[[2025,6,30]],"date-time":"2025-06-30T18:04:53Z","timestamp":1751306693000},"page":"559-566","update-policy":"https:\/\/doi.org\/10.25259\/crossmark_policy","source":"Crossref","is-referenced-by-count":0,"title":["Investigation of human herpesvirus 8 & Leishmania<\/i> species in malignant skin tumours, psoriasis, actinic keratoses, & seborrheic keratoses: A single-center experience from Ankara, Turkey"],"prefix":"10.25259","volume":"161","author":[{"given":"Ayfer","family":"Bakir","sequence":"first","affiliation":[{"name":"Department of Microbiology, Ministry of Health, Ankara Etlik City Hospital, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selma","family":"Usluca","sequence":"additional","affiliation":[{"name":"Department of Microbiology, Atilim University Faculty of Medicine, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Selda Pelin","family":"Kartal","sequence":"additional","affiliation":[{"name":"Department of Dermatology and Venerology, Ministry of Health, Ankara Etlik City Hospital, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Murat","family":"Alper","sequence":"additional","affiliation":[{"name":"Department of Pathology, Ministry of Health, Ankara Etlik City Hospital, Ankara, Turkey"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"10894","published-online":{"date-parts":[[2025,6,26]]},"reference":[{"key":"10.25259\/IJMR_849_2024\/ref-1","doi-asserted-by":"crossref","first-page":"665","DOI":"10.3390\/biomedicines13030665","article-title":"Small biological 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