{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T22:59:55Z","timestamp":1792450795370,"version":"4.5.0"},"reference-count":46,"publisher":"Oxford University Press (OUP)","issue":"9","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2004,9,1]]},"DOI":"10.2527\/2004.8292693x","type":"journal-article","created":{"date-parts":[[2016,8,9]],"date-time":"2016-08-09T21:10:55Z","timestamp":1470777055000},"page":"2693-2704","source":"Crossref","is-referenced-by-count":73,"title":["In vitro effects of individual fatty acids on protozoal numbers and on fermentation products in ruminal fluid from cattle fed a high-concentrate, barley-based diet12"],"prefix":"10.1093","volume":"82","author":[{"given":"A. N.","family":"Hristov","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Ivan","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"T. A.","family":"McAllister","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","reference":[{"key":"key\n\t\t\t\t20180202040843_AJISAKA-ETAL-2002","doi-asserted-by":"crossref","first-page":"479","DOI":"10.1046\/j.1344-3941.2002.00066.x","article-title":"Effects of medium-chain fatty acid-cyclodextrin complexes on ruminal methane production in vitro","volume":"73","author":"Ajisaka","year":"2002","journal-title":"Anim. Sci. J."},{"key":"key\n\t\t\t\t20180202040843_BRODERICK-AND-KANG-1980","doi-asserted-by":"crossref","first-page":"64","DOI":"10.3168\/jds.S0022-0302(80)82888-8","article-title":"Automated simultaneous determination of ammonia and total amino acids in ruminal fluid and in vitro media","volume":"63","author":"Broderick","year":"1980","journal-title":"J. Dairy Sci."},{"key":"key\n\t\t\t\t20180202040843_BROOKS-ETAL-1954","doi-asserted-by":"crossref","first-page":"758","DOI":"10.2527\/jas1954.134758x","article-title":"The effect of added fat on the digestion of cellulose and protein by ovine rumen microorganisms","volume":"13","author":"Brooks","year":"1954","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_CAMPBELL-ETAL-1982","doi-asserted-by":"crossref","first-page":"535","DOI":"10.1080\/00288233.1982.10425218","article-title":"An in vitro assay for compounds toxic to rumen protozoa","volume":"25","author":"Campbell","year":"1982","journal-title":"N. Z. J. Agric. Res."},{"key":"key\n\t\t\t\t20180202040843_CCAC-1993","volume-title":"Guide to the Care and Use of Experimental Animals.","author":"CCAC","year":"1993","edition":"2nd ed"},{"key":"key\n\t\t\t\t20180202040843_CRC-1988","volume-title":"CRC Handbook of Chemistry and Physics.","author":"CRC","year":"1988","edition":"69th ed"},{"key":"key\n\t\t\t\t20180202040843_DOHME-ETAL-1999","doi-asserted-by":"crossref","first-page":"187","DOI":"10.1046\/j.1365-2672.1999.00614.x","article-title":"The role of the rumen ciliate protozoa for methane suppression caused by coconut oil","volume":"29","author":"Dohme","year":"1999","journal-title":"Lett. Appl. Microbiol."},{"key":"key\n\t\t\t\t20180202040843_DOREAU-ETAL-1991","doi-asserted-by":"crossref","first-page":"2233","DOI":"10.3168\/jds.S0022-0302(91)78396-3","article-title":"Effect of source and level of supplemental fat on total and ruminal organic matter and nitrogen digestion in dairy cows","volume":"74","author":"Doreau","year":"1991","journal-title":"J. Dairy Sci."},{"key":"key\n\t\t\t\t20180202040843_DOREAU-AND-FERLAY-1995","doi-asserted-by":"crossref","first-page":"97","DOI":"10.1016\/0301-6226(95)00041-I","article-title":"Effect of dietary lipids on nitrogen metabolism in the rumen: A review","volume":"43","author":"Doreau","year":"1995","journal-title":"Livest. Prod. Sci."},{"key":"key\n\t\t\t\t20180202040843_FIRKINS-1996","doi-asserted-by":"crossref","first-page":"1347S","DOI":"10.1093\/jn\/126.suppl_4.1347S","article-title":"Maximizing microbial protein synthesis in the rumen","volume":"126","author":"Firkins","year":"1996","journal-title":"J. Nutr."},{"key":"key\n\t\t\t\t20180202040843_GOODALL-AND-BYERS-1978","doi-asserted-by":"crossref","first-page":"80","DOI":"10.1016\/0003-2697(78)90728-5","article-title":"Automated micro method for enzymatic L(+) and D(() lactic acid determination in biological fluids containing cellular extracts","volume":"89","author":"Goodall","year":"1978","journal-title":"Anal. Biochem."},{"key":"key\n\t\t\t\t20180202040843_HARFOOT-AND-HAZLEWOOD-1997","doi-asserted-by":"crossref","DOI":"10.1007\/978-94-009-1453-7_9","article-title":"Lipid metabolism in the rumen","volume-title":"Rumen Microbial Ecosystem.","author":"Harfoot","year":"1997"},{"key":"key\n\t\t\t\t20180202040843_HARFOOT-ETAL-1974","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1111\/j.1365-2672.1974.tb00487.x","article-title":"Competition between food particles and rumen bacteria in the uptake of long-chain fatty acids and triglycerides","volume":"37","author":"Harfoot","year":"1974","journal-title":"J. Appl. Bact."},{"key":"key\n\t\t\t\t20180202040843_HAWKE-AND-ROBERTSON-1964","doi-asserted-by":"crossref","first-page":"283","DOI":"10.1002\/jsfa.2740150503","article-title":"Studies on rumen metabolism. II. In vivo hydrolysis and hydrogenation of lipid","volume":"15","author":"Hawke","year":"1964","journal-title":"J. Sci. Food Agric."},{"key":"key\n\t\t\t\t20180202040843_HENDERSON-1973","doi-asserted-by":"crossref","first-page":"107","DOI":"10.1017\/S0021859600058378","article-title":"The effects of fatty acids on pure cultures of rumen bacteria","volume":"81","author":"Henderson","year":"1973","journal-title":"J. Agric. Sci. (Camb.)"},{"key":"key\n\t\t\t\t20180202040843_HOOGENRAAD-AND-HIRD-1970","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1099\/00221287-62-2-261","article-title":"Factors concerned in the lysis of bacteria in the alimentary tract of sheep","volume":"62","author":"Hoogenraad","year":"1970","journal-title":"J. Gen. Microbiol."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-2000","unstructured":"Hristov, A. N. 2000. Carbohydrate-protein interactions in the rumen and possibilities of improving the efficiency of ammonia-N utilization in dairy cows. Pages 51\u201371 in Proc. Pacific Northwest Anim. Nutr. Conf., Spokane, WA."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-AND-BRODERICK-1994","doi-asserted-by":"crossref","first-page":"1344","DOI":"10.2527\/1994.7251344x","article-title":"In vitro determination of ruminal protein degradability using [15]N-ammonia to correct for microbial nitrogen uptake","volume":"72","author":"Hristov","year":"1994","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-ETAL-2003","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1016\/S0377-8401(03)00060-9","article-title":"Evaluation of several potential bioactive agents for reducing protozoal activity in vitro","volume":"105","author":"Hristov","year":"2003","journal-title":"Anim. Feed Sci. Technol."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-ETAL-2001","doi-asserted-by":"crossref","first-page":"515","DOI":"10.2527\/2001.792515x","article-title":"Fermentation characteristics and ruminal ciliate protozoal populations in cattle fed medium- or high-concentrate barley-based diets","volume":"79","author":"Hristov","year":"2001","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-ETAL-1998","doi-asserted-by":"crossref","first-page":"3146","DOI":"10.2527\/1998.76123146x","article-title":"Effect of dietary or abomasal supplementation of exogenous polysaccharide-degrading enzymes on rumen fermentation and nutrient digestibility","volume":"76","author":"Hristov","year":"1998","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-ETAL-1999","doi-asserted-by":"crossref","first-page":"2554","DOI":"10.2527\/1999.7792554x","article-title":"Effect of Yucca schidigera extract on rumen fermentation and nutrient digestion in heifers","volume":"77","author":"Hristov","year":"1999","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_HRISTOV-ETAL-2002","doi-asserted-by":"crossref","first-page":"1886","DOI":"10.1002\/jsfa.1238","article-title":"Proteolytic activity in ruminal fluid from cattle fed two levels of barley grain: A comparison of three methods of determination","volume":"82","author":"Hristov","year":"2002","journal-title":"J. Sci. Food Agric."},{"key":"key\n\t\t\t\t20180202040843_JARVIS-1968","doi-asserted-by":"crossref","first-page":"714","DOI":"10.1128\/am.16.5.714-723.1968","article-title":"Lysis of viable rumen bacteria in bovine rumen fluid","volume":"16","author":"Jarvis","year":"1968","journal-title":"Appl. Microbiol."},{"key":"key\n\t\t\t\t20180202040843_JENKINS-1993","doi-asserted-by":"crossref","first-page":"3851","DOI":"10.3168\/jds.S0022-0302(93)77727-9","article-title":"Lipid metabolism in the rumen","volume":"76","author":"Jenkins","year":"1993","journal-title":"J. Dairy Sci."},{"key":"key\n\t\t\t\t20180202040843_JOUANY-1996","doi-asserted-by":"crossref","first-page":"1335S","DOI":"10.1093\/jn\/126.suppl_4.1335S","article-title":"Effect of rumen protozoa on nitrogen utilization by ruminants","volume":"126","author":"Jouany","year":"1996","journal-title":"J. Nutr."},{"key":"key\n\t\t\t\t20180202040843_MACHMULLER-AND-KREUZER-1999","doi-asserted-by":"crossref","first-page":"65","DOI":"10.4141\/A98-079","article-title":"Methane suppression by coconut oil and associated effects on nutrient and energy balance in sheep","volume":"79","author":"Machmu\u0308ller","year":"1999","journal-title":"Can. J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_MACRAE-ETAL-1995","volume-title":"Protein metabolism in specific organs.","author":"MacRae","year":"1995"},{"key":"key\n\t\t\t\t20180202040843_MACZULAK-ETAL-1981","doi-asserted-by":"crossref","first-page":"856","DOI":"10.1128\/aem.42.5.856-862.1981","article-title":"Effects of long-chain fatty acids on growth of rumen bacteria","volume":"42","author":"Maczulak","year":"1981","journal-title":"Appl. Environ. Microbiol."},{"key":"key\n\t\t\t\t20180202040843_MCDOUGALL-1948","doi-asserted-by":"crossref","first-page":"99","DOI":"10.1042\/bj0430099","article-title":"The composition and output of sheep's saliva","volume":"43","author":"McDougall","year":"1948","journal-title":"Biochem. J."},{"key":"key\n\t\t\t\t20180202040843_MATSUMOTO-ETAL-1991","doi-asserted-by":"crossref","first-page":"439","DOI":"10.2323\/jgam.37.439","article-title":"Defaunation effects of medium-chain fatty acids and their derivatives on goat rumen protozoa","volume":"37","author":"Matsumoto","year":"1991","journal-title":"J. Gen. Appl. Microbiol."},{"key":"key\n\t\t\t\t20180202040843_NAGARAJA-ETAL-1997","doi-asserted-by":"crossref","DOI":"10.1007\/978-94-009-1453-7_13","article-title":"Manipulation of ruminal fermentation","volume-title":"Rumen Microbial Ecosystem.","author":"Nagaraja","year":"1997"},{"key":"key\n\t\t\t\t20180202040843_NEWBOLD-AND-CHAMBERLAIN-1988","first-page":"154A","article-title":"Lipids as rumen-defaunating agents","volume":"43","author":"Newbold","year":"1988","journal-title":"Proc. Nutr. Soc."},{"key":"key\n\t\t\t\t20180202040843_NEWBOLD-ETAL-1997","doi-asserted-by":"crossref","first-page":"237","DOI":"10.1079\/BJN19970143","article-title":"Influence of foliage from African multipurpose trees on activity of rumen protozoa and bacteria","volume":"78","author":"Newbold","year":"1997","journal-title":"Br. J. Nutr."},{"key":"key\n\t\t\t\t20180202040843_NEWBOLD-ETAL-1987","doi-asserted-by":"crossref","first-page":"9","DOI":"10.1002\/jsfa.2740380104","article-title":"The in vitro metabolism of D,L-lactic acid by rumen microorganisms","volume":"38","author":"Newbold","year":"1987","journal-title":"J. Sci. Food Agric."},{"key":"key\n\t\t\t\t20180202040843_OGIMOTO-AND-IMAI-1981","volume-title":"Atlas of Rumen Microbiology.","author":"Ogimoto","year":"1981"},{"key":"key\n\t\t\t\t20180202040843_OLDICK-AND-FIRKINS-2000","doi-asserted-by":"crossref","first-page":"2412","DOI":"10.2527\/2000.7892412x","article-title":"Effects of degree of fat saturation on fiber digestion and microbial protein synthesis when diets are fed twelve times daily","volume":"78","author":"Oldick","year":"2000","journal-title":"J. Anim. Sci."},{"key":"key\n\t\t\t\t20180202040843_OOSTA-ETAL-1978","doi-asserted-by":"crossref","first-page":"31","DOI":"10.1016\/0003-2697(78)90723-6","article-title":"Optimization of Folin-Ciocalteu reagent concentration in an automated Lowry protein assay","volume":"89","author":"Oosta","year":"1978","journal-title":"Anal. Biochem."},{"key":"key\n\t\t\t\t20180202040843_PANTOJA-ETAL-1994","doi-asserted-by":"crossref","first-page":"2341","DOI":"10.3168\/jds.S0022-0302(94)77177-0","article-title":"Effects of fat saturation and source of fiber on site and nutrient digestion and milk production by lactating dairy cows","volume":"77","author":"Pantoja","year":"1994","journal-title":"J. Dairy Sci."},{"key":"key\n\t\t\t\t20180202040843_SUTTON-ETAL-1983","doi-asserted-by":"crossref","first-page":"419","DOI":"10.1079\/BJN19830051","article-title":"Digestion and synthesis in the rumen of sheep given diets supplemented with free and protected oils","volume":"49","author":"Sutton","year":"1983","journal-title":"Br. J. Nutr."},{"key":"key\n\t\t\t\t20180202040843_TAMMINGA-1992","doi-asserted-by":"crossref","first-page":"345","DOI":"10.3168\/jds.S0022-0302(92)77770-4","article-title":"Nutrition management of dairy cows as a contribution to pollution control","volume":"75","author":"Tamminga","year":"1992","journal-title":"J. Dairy Sci."},{"key":"key\n\t\t\t\t20180202040843_TESFA-1993","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1016\/0377-8401(93)90005-5","article-title":"Effects of rape-seed oil supplementation on digestion, microbial protein synthesis and duodenal microbial amino acid composition in ruminants","volume":"41","author":"Tesfa","year":"1993","journal-title":"Anim. Feed Sci. Technol."},{"key":"key\n\t\t\t\t20180202040843_WALLACE-1994","article-title":"Amino acid and protein synthesis, turnover, and breakdown by ruminal microorganisms","volume-title":"Principles of Protein Nutrition of Ruminants.","author":"Wallace","year":"1994"},{"key":"key\n\t\t\t\t20180202040843_WALLACE-AND-MCPHERSON-1987","doi-asserted-by":"crossref","first-page":"313","DOI":"10.1079\/BJN19870098","article-title":"Factors affecting the rate of breakdown of bacterial protein in rumen fluid","volume":"58","author":"Wallace","year":"1987","journal-title":"Br. J. Nutr."},{"key":"key\n\t\t\t\t20180202040843_WALLACE-AND-NEWBOLD-1991","doi-asserted-by":"crossref","first-page":"163","DOI":"10.1017\/S0021859600076279","article-title":"Effects of bentonite on fermentation in the rumen simulation technique (Rusitec) and on rumen ciliate protozoa","volume":"116","author":"Wallace","year":"1991","journal-title":"J. Agric. Sci. (Camb.)"},{"key":"key\n\t\t\t\t20180202040843_WILLIAMS-AND-COLEMAN-1992","doi-asserted-by":"crossref","DOI":"10.1007\/978-1-4612-2776-2","volume-title":"The Rumen Protozoa.","author":"Williams","year":"1992"}],"container-title":["Journal of Animal Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/jas\/article-pdf\/82\/9\/2693\/23751597\/2693.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,7,5]],"date-time":"2022-07-05T15:29:03Z","timestamp":1657034943000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/jas\/article\/82\/9\/2693\/4790595"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2004,9,1]]},"references-count":46,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2004,9,1]]}},"URL":"https:\/\/doi.org\/10.2527\/2004.8292693x","relation":{},"ISSN":["0021-8812","1525-3163"],"issn-type":[{"value":"0021-8812","type":"print"},{"value":"1525-3163","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2004,9]]},"published":{"date-parts":[[2004,9,1]]}}}