{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,2]],"date-time":"2026-08-02T21:33:05Z","timestamp":1785706385121,"version":"3.56.0"},"reference-count":0,"publisher":"Swiss Chemical Society","issue":"1-2","license":[{"start":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T00:00:00Z","timestamp":1645574400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chimia"],"abstract":"<jats:p>Gangliosides are a family of conjugates consisting of a polar sialoglycan head group and a hydrophobic\u00a0ceramide tail. Gangliosides are of high abundance in neuronal tissues and are involved in numerous biological\u00a0processes, such as cell-cell recognition, adhesion, and signal transduction. Alteration of the ganglioside profile\u00a0is associated with various neurodegenerative diseases and there is indication that gangliosides are involved in\u00a0the pathogenesis of Parkinson\u2019s and Huntington\u2019s disease. The development of refined methods for the analysis\u00a0of gangliosides by high-performance liquid chromatography coupled to mass spectrometry (HPLC-MS) has\u00a0supported research with qualitative and quantitative data. However, the amphiphilic character of gangliosides\u00a0renders their separation and mass spectrometric analysis challenging. In this article, the strengths of hydrophilic\u00a0interaction liquid chromatography (HILIC) for baseline separation of gangliosides, including two structural isomers,\u00a0and their structural characterization by tandem mass spectrometry are demonstrated. The importance\u00a0of ion source parameter optimization is highlighted to prevent misleading ganglioside transformation due to\u00a0in-source dissociation.<\/jats:p>","DOI":"10.2533\/chimia.2022.109","type":"journal-article","created":{"date-parts":[[2022,2,23]],"date-time":"2022-02-23T07:15:08Z","timestamp":1645600508000},"page":"109","source":"Crossref","is-referenced-by-count":7,"title":["Challenges in the Analysis of Gangliosides by LC-MS"],"prefix":"10.2533","volume":"76","author":[{"given":"Thomas","family":"Muggli","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia","family":"B\u00fchr","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Stefan","family":"Sch\u00fcrch","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1442","published-online":{"date-parts":[[2022,2,23]]},"container-title":["CHIMIA"],"original-title":[],"link":[{"URL":"https:\/\/chimia.ch\/chimia\/article\/download\/2022_109\/5197","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/chimia.ch\/chimia\/article\/download\/2022_109\/5197","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,9,19]],"date-time":"2022-09-19T14:02:36Z","timestamp":1663596156000},"score":1,"resource":{"primary":{"URL":"https:\/\/chimia.ch\/chimia\/article\/view\/2022_109"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,2,23]]},"references-count":0,"journal-issue":{"issue":"1-2","published-online":{"date-parts":[[2022,2,23]]}},"URL":"https:\/\/doi.org\/10.2533\/chimia.2022.109","relation":{},"ISSN":["2673-2424","0009-4293"],"issn-type":[{"value":"2673-2424","type":"electronic"},{"value":"0009-4293","type":"print"}],"subject":[],"published":{"date-parts":[[2022,2,23]]}}}