{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,22]],"date-time":"2026-09-22T13:43:38Z","timestamp":1790084618100,"version":"4.0.1"},"reference-count":0,"publisher":"Swiss Chemical Society","issue":"3","license":[{"start":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T00:00:00Z","timestamp":1742947200000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Chimia"],"abstract":"<jats:p>Glycosylation is a profound influencer of glycoprotein function. Glycans have a critical impact on health and disease, yet the tools to study them have trailed behind proteins and nucleic acids. O-GalNAc glycosylation involves the addition of N-acetylgalactosamine (GalNAc) to protein substrates. Dysregulation of O-GalNAc glycosylation is implicated in many pathologies such as cancer. Studying O-GalNAc glycosylation is complicated by the lack of a consensus sequence for initiation and the complex interdependence between a large family of 20 GalNAc transferases (GalNAc-Ts) in human cells. These issues necessitate precise methods of interrogating enzyme function. Herein, we discuss our own advances into the generation of precision tools to study O-GalNAc glycosylation and other glycosylation types. We discuss the use of bump-and-hole engineering to illuminate the roles of individual GalNAc-Ts. Engineering biosynthetic pathways enables cell line-specific uptake of chemical, editable sugars in co-culture settings. We provide an insight into the state-of-the-art in this field.<\/jats:p>","DOI":"10.2533\/chimia.2025.146","type":"journal-article","created":{"date-parts":[[2025,3,26]],"date-time":"2025-03-26T01:28:05Z","timestamp":1742952485000},"page":"146-151","source":"Crossref","is-referenced-by-count":2,"title":["Precision Probing of O-GalNAc Glycosylation Using Bump-and-Hole Engineering"],"prefix":"10.2533","volume":"79","author":[{"given":"Abdul","family":"Zafar","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Benjamin","family":"Schumann","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"1442","published-online":{"date-parts":[[2025,3,26]]},"container-title":["CHIMIA"],"original-title":[],"link":[{"URL":"https:\/\/www.chimia.ch\/chimia\/article\/download\/2025_146\/2025_146","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/www.chimia.ch\/chimia\/article\/download\/2025_146\/2025_146","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,3,29]],"date-time":"2025-03-29T13:54:19Z","timestamp":1743256459000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.chimia.ch\/chimia\/article\/view\/2025_146"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,3,26]]},"references-count":0,"journal-issue":{"issue":"3","published-online":{"date-parts":[[2025,3,26]]}},"URL":"https:\/\/doi.org\/10.2533\/chimia.2025.146","relation":{},"ISSN":["2673-2424","0009-4293"],"issn-type":[{"value":"2673-2424","type":"electronic"},{"value":"0009-4293","type":"print"}],"subject":[],"published":{"date-parts":[[2025,3,26]]}}}