{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,8,31]],"date-time":"2025-08-31T10:30:10Z","timestamp":1756636210764,"version":"3.37.3"},"reference-count":83,"publisher":"Consilium Medicum","issue":"4","license":[{"start":{"date-parts":[[2020,4,19]],"date-time":"2020-04-19T00:00:00Z","timestamp":1587254400000},"content-version":"unspecified","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc-sa\/4.0"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["Terapevticheskii arkhiv"],"abstract":"<jats:p>Cardiovascular diseases are the leading cause of morbidity and mortality worldwide. In recent years, researchers are attracted to the use of cell therapy based on stem cell and progenitor cells, which has been a promising strategy for cardiac repair after injury. However, conducted research using intracoronary or intramyocardial transplantation of various types of stem\/progenitor cells as a cell suspension showed modest efficiency. This is due to the low degree of integration and cell survival after transplantation. To overcome these limitations, the concept of the use of multicellular spheroids modeling the natural microenvironment of cells has been proposed, which allows maintaining their viability and therapeutic properties. It is of great interest to use so-called cardial spheroids (cardiospheres)  spontaneously forming three-dimensional structures under low-adhesive conditions, consisting of a heterogeneous population of myocardial progenitor cells and extracellular matrix proteins. This review presents data on methods for creating cardiospheres, directed regulation of their properties and reparative potential, as well as the results of preclinical and clinical studies on their use for the treatment of heart diseases.<\/jats:p>","DOI":"10.26442\/00403660.2020.04.000634","type":"journal-article","created":{"date-parts":[[2020,6,29]],"date-time":"2020-06-29T14:35:20Z","timestamp":1593441320000},"page":"111-120","source":"Crossref","is-referenced-by-count":8,"title":["Perspectives of cell therapy for myocardial infarction and heart failure based on cardiosphere cells"],"prefix":"10.26442","volume":"92","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2712-4997","authenticated-orcid":false,"given":"K. V.","family":"Dergilev","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6367-3785","authenticated-orcid":false,"given":"Iu. D.","family":"Vasilets","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2441-6062","authenticated-orcid":false,"given":"Z. I.","family":"Tsokolaeva","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0512-3670","authenticated-orcid":false,"given":"E. S.","family":"Zubkova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0969-5780","authenticated-orcid":false,"given":"E. V.","family":"Parfenova","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"18344","published-online":{"date-parts":[[2020,4,19]]},"reference":[{"key":"ref1","doi-asserted-by":"publisher","DOI":"10.1097\/CRD.0000000000000173"},{"key":"ref2","doi-asserted-by":"publisher","DOI":"10.1155\/2012\/354605"},{"key":"ref3","doi-asserted-by":"publisher","DOI":"10.4155\/fmc.15.107"},{"key":"ref4","doi-asserted-by":"publisher","DOI":"10.33594\/000000180"},{"key":"ref5","doi-asserted-by":"publisher","DOI":"10.1038\/s41569-018-0013-0"},{"key":"ref6","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2015.11.036"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Afzal MR, Samanta A, Shah ZI, et al. Adult Bone Marrow Cell Therapy for Ischemic Heart Disease: Evidence and Insights From Randomized Controlled Trials. Circ Res. 2015;117(6):558-75. doi: 10.1161\/ CIRCRESAHA.114.304792","DOI":"10.1161\/CIRCRESAHA.114.304792"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Gil-Perot\u00edn S, Duran-Moreno M, Cebri\u00e1n-Silla A, et al. Adult neural stem cells from the subventricular zone: a review of the neurosphere assay. Anat Rec (Hoboken). 2013;296(9):1435-52. doi: 10.1002\/ ar.22746","DOI":"10.1002\/ar.22746"},{"key":"ref9","doi-asserted-by":"publisher","DOI":"10.1038\/nrm1858"},{"key":"ref10","doi-asserted-by":"publisher","DOI":"10.1038\/nrm2236"},{"key":"ref11","doi-asserted-by":"publisher","DOI":"10.1007\/s10439-005-8159-4"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Gallet R, Tseliou E, Dawkins J, et al. Intracoronary delivery of self-assembling heart-derived microtissues (cardiospheres) for prevention of adverse remodeling in a pig model of convalescent myocardial infarction. Circ Cardiovasc Interv. 2015;8(5):e002391. doi: 10.1161\/ CIRCINTERVENTIONS.115.002391","DOI":"10.1161\/CIRCINTERVENTIONS.115.002391"},{"key":"ref13","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts"},{"key":"ref14","doi-asserted-by":"publisher","DOI":"10.3109\/08977190903512628"},{"key":"ref15","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.12210"},{"key":"ref16","doi-asserted-by":"publisher","DOI":"10.1161\/01.RES.0000147315.71699.51"},{"key":"ref17","unstructured":"Fabrizi C, Angelini F, Chimenti I, et al. Thrombin and thrombin-derived peptides promote proliferation of cardiac progenitor cells in the form of cardiospheres without affecting their differentiation potential. J Biol Regul Homeost Agents. 2011;25(2 Suppl.):43-51."},{"key":"ref18","doi-asserted-by":"publisher","DOI":"10.1007\/s12265-017-9741-5"},{"key":"ref19","doi-asserted-by":"publisher","DOI":"10.1177\/026119290203000208"},{"key":"ref20","doi-asserted-by":"publisher","DOI":"10.1002\/jcp.25797"},{"key":"ref21","doi-asserted-by":"publisher","DOI":"10.3390\/cancers9080098"},{"key":"ref22","doi-asserted-by":"publisher","DOI":"10.1089\/scd.2012.0277"},{"key":"ref23","doi-asserted-by":"publisher","DOI":"10.1111\/jcmm.13832"},{"key":"ref24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0037800"},{"key":"ref25","doi-asserted-by":"publisher","DOI":"10.1016\/j.yjmcc.2008.02.273"},{"key":"ref26","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.110.228809"},{"key":"ref27","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0500169102"},{"key":"ref28","doi-asserted-by":"publisher","DOI":"10.1038\/srep36774"},{"key":"ref29","doi-asserted-by":"crossref","unstructured":"Amirrasouli MM, Shamsara M. Comparing the in vivo and in vitro effects of hypoxia (3% O2) on directly derived cells from murine cardiac explants versus murine cardiosphere derived cells. J Stem Cells Regen Med. 2017;13(2):35-44.","DOI":"10.46582\/jsrm.1302007"},{"key":"ref30","doi-asserted-by":"crossref","unstructured":"Gonzalez DM, Medici D. Signaling mechanisms of the epithelial-mesenchymal transition. Sci Signal. 2014;7(344):re8. doi: 10.1126\/ scisignal.2005189","DOI":"10.1126\/scisignal.2005189"},{"key":"ref31","doi-asserted-by":"publisher","DOI":"10.3727\/096368915X687697"},{"key":"ref32","unstructured":"Hosoyama T, Samura M, Kudo T, et al. Cardiosphere-derived cell sheet primed with hypoxia improves left ventricular function of chronically infarcted heart. Am J Transl Res. 2015;7(12):2738-51."},{"key":"ref33","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.109.210682"},{"key":"ref34","doi-asserted-by":"publisher","DOI":"10.1002\/stem.532"},{"key":"ref35","doi-asserted-by":"publisher","DOI":"10.1038\/mt.2012.109"},{"key":"ref36","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2017.04.015"},{"key":"ref37","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2013.09.013"},{"key":"ref38","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2010.07.049"},{"key":"ref39","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0144523"},{"key":"ref40","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.816058"},{"key":"ref41","doi-asserted-by":"publisher","DOI":"10.1016\/j.biomaterials.2012.04.006"},{"key":"ref42","doi-asserted-by":"crossref","unstructured":"Tanaka Y, Hosoyama T, Mikamo A, et al. Hypoxic preconditioning of human cardiosphere-derived cell sheets enhances cellular functions via activation of the PI3K\/Akt\/mTOR\/HIF-1\u03b1 pathway. Am J Transl Res. 2017;9(2):664-73.","DOI":"10.20944\/preprints201608.0124.v1"},{"key":"ref43","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0149001"},{"key":"ref44","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehw240"},{"key":"ref45","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.125754"},{"key":"ref46","doi-asserted-by":"publisher","DOI":"10.1016\/j.expneurol"},{"key":"ref47","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2015.05.068"},{"key":"ref48","doi-asserted-by":"publisher","DOI":"10.1093\/eurheartj\/ehx454"},{"key":"ref49","doi-asserted-by":"publisher","DOI":"10.1161\/HYPERTENSIONAHA.118.11239"},{"key":"ref50","doi-asserted-by":"publisher","DOI":"10.1016\/j.stemcr.2014.04.006"},{"key":"ref51","doi-asserted-by":"crossref","unstructured":"Malliaras K, Li TS, Luthringer D, et al. Safety and efficacy of allogeneic cell therapy in infarcted rats transplanted with mismatched cardiosphere-derived cells. Circulation. 2012;125(1):10-2. doi: 10.1161\/ CIRCULATIONAHA.111.042598","DOI":"10.1161\/CIRCULATIONAHA.111.042598"},{"key":"ref52","doi-asserted-by":"publisher","DOI":"10.1016\/j.tvjl.2010.12.033"},{"key":"ref53","doi-asserted-by":"publisher","DOI":"10.1016\/s0735-1097(99)00630-0"},{"key":"ref54","doi-asserted-by":"crossref","unstructured":"Wu QQ, Xiao Y, Yuan Y, et al. Mechanisms contributing to cardiac remodelling. Clinical Science. 2017;131(18):2319-45. doi: 10.1042\/ cs20171167","DOI":"10.1042\/CS20171167"},{"key":"ref55","doi-asserted-by":"publisher","DOI":"10.1113\/JP275548"},{"key":"ref56","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0113805"},{"key":"ref57","doi-asserted-by":"publisher","DOI":"10.1007\/s00395-014-0443-8"},{"key":"ref58","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0088590"},{"key":"ref59","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0007195"},{"key":"ref60","doi-asserted-by":"crossref","unstructured":"Kanazawa H, Tseliou E, Dawkins JF, et al. Durable Benefits of Cellular Postconditioning: Long-Term Effects of Allogeneic Cardiosphere-Derived Cells Infused After Reperfusion in Pigs with Acute Myocardial Infarction. J Am Heart Assoc. 2016;5(2):e002796. doi: 10.1161\/ JAHA.115.002796","DOI":"10.1161\/JAHA.115.002796"},{"key":"ref61","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2012.10.052"},{"key":"ref62","doi-asserted-by":"publisher","DOI":"10.1016\/j.it.2015.11.005"},{"key":"ref63","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.112.276501"},{"key":"ref64","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacbts.2016.01.003"},{"key":"ref65","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-018-31569-1"},{"key":"ref66","doi-asserted-by":"publisher","DOI":"10.1172\/JCI81321"},{"key":"ref67","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pone.0165255"},{"key":"ref68","doi-asserted-by":"publisher","DOI":"10.1177\/1074248418784287"},{"key":"ref69","doi-asserted-by":"publisher","DOI":"10.5339\/gcsp.2013.37"},{"key":"ref70","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2013.08.724"},{"key":"ref71","doi-asserted-by":"publisher","DOI":"10.1155\/2013\/784541"},{"key":"ref72","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2018.09.117"},{"key":"ref73","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.109.871905"},{"key":"ref74","doi-asserted-by":"publisher","DOI":"10.1002\/stem.1736"},{"key":"ref75","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvu167"},{"key":"ref76","doi-asserted-by":"crossref","unstructured":"Jafarzadeh M, Mohammad Soltani B, Ekhteraei Tousi S, Behmanesh M. Hsa-miR-497 as a new regulator in TGF\u03b2 signaling pathway and cardiac differentiation process. Gene. 2018;675:150-6. doi: 10.1016\/ j.gene.2018.06.098","DOI":"10.1016\/j.gene.2018.06.098"},{"key":"ref77","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(12)60195-0"},{"key":"ref78","doi-asserted-by":"crossref","unstructured":"Tarui S, Ishigami S, Ousaka D, et al. Transcoronary infusion of cardiac progenitor cells in hypoplastic left heart syndrome: Three-year follow-up of the Transcoronary Infusion of Cardiac Progenitor Cells in Patients With Single-Ventricle Physiology (TICAP) trial. J Thorac Cardiovasc Surg. 2015;150(5):1198-207,1208.e1-2. doi: 10.1016\/ j.jtcvs.2015.06.076","DOI":"10.1016\/j.jtcvs.2015.06.076"},{"key":"ref79","doi-asserted-by":"publisher","DOI":"10.3727\/096368916X692933"},{"key":"ref80","doi-asserted-by":"publisher","DOI":"10.1016\/j.jacc.2016.09.848"},{"key":"ref81","doi-asserted-by":"publisher","DOI":"10.1212\/WNL.0000000000006950"},{"key":"ref82","doi-asserted-by":"publisher","DOI":"10.4252\/wjsc.v9.i12.203"},{"key":"ref83","doi-asserted-by":"publisher","DOI":"10.1016\/j.scr.2018.08.015"}],"container-title":["Terapevticheskii arkhiv"],"original-title":[],"link":[{"URL":"https:\/\/ter-arkhiv.ru\/0040-3660\/article\/viewFile\/34117\/22450","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/ter-arkhiv.ru\/0040-3660\/article\/viewFile\/34117\/22450","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,10,13]],"date-time":"2021-10-13T20:48:47Z","timestamp":1634158127000},"score":1,"resource":{"primary":{"URL":"https:\/\/ter-arkhiv.ru\/0040-3660\/article\/view\/34117"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2020,4,19]]},"references-count":83,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2020,4,19]]}},"URL":"https:\/\/doi.org\/10.26442\/00403660.2020.04.000634","relation":{},"ISSN":["2309-5342","0040-3660"],"issn-type":[{"type":"electronic","value":"2309-5342"},{"type":"print","value":"0040-3660"}],"subject":[],"published":{"date-parts":[[2020,4,19]]}}}