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Alliance"],"published-print":{"date-parts":[[2019,4]]},"abstract":"<jats:p>Upon tumor development, new extracellular vesicles appear in circulation. Our knowledge of their relative abundance, function, and overall impact on cancer development is still preliminary. Here, we demonstrate that plasma extracellular vesicles (pEVs) of non-tumor origin are persistently increased in untreated and post-excision melanoma patients, exhibiting strong suppressive effects on the proliferation of tumor cells. Plasma vesicle numbers, miRNAs, and protein levels were elevated two- to tenfold and detected many years after tumor resection. The vesicles revealed individual and clinical stage-specific miRNA profiles as well as active ADAM10. However, whereas pEV from patients preventing tumor relapse down-regulated \u03b2-catenin and blocked tumor cell proliferation in an miR-34a\u2013dependent manner, pEV from metastatic patients lost this ability and stimulated \u03b2-catenin\u2013mediated transcription. 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