{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T18:51:34Z","timestamp":1791312694044,"version":"4.3.1"},"reference-count":61,"publisher":"Life Science Alliance, LLC","issue":"1","license":[{"start":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T00:00:00Z","timestamp":1576454400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100004543","name":"China Scholarship Council","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100004543","id-type":"DOI","asserted-by":"crossref"}]},{"name":"American Heart Association Scientist Development","award":["15SDG25590001"],"award-info":[{"award-number":["15SDG25590001"]}]},{"name":"Progenitor Cell Biology Consortium \u201cJUMP START\u201d AWARDS","award":["5U01HL099997-07"],"award-info":[{"award-number":["5U01HL099997-07"]}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["HL138454-01"],"award-info":[{"award-number":["HL138454-01"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"crossref","award":["R01 HL116461"],"award-info":[{"award-number":["R01 HL116461"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"crossref"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"published-print":{"date-parts":[[2020,1]]},"abstract":"<jats:p>Myocardial recovery from ischemia-reperfusion (IR) is shaped by the interaction of many signaling pathways and tissue repair processes, including the innate immune response. We and others previously showed that sustained expression of the transcriptional co-activator yes-associated protein (YAP) improves survival and myocardial outcome after myocardial infarction. Here, we asked whether transient YAP expression would improve myocardial outcome after IR injury. After IR, we transiently activated YAP in the myocardium with modified mRNA encoding a constitutively active form of YAP (aYAP modRNA). Histological studies 2 d after IR showed that aYAP modRNA reduced cardiomyocyte (CM) necrosis and neutrophil infiltration. 4 wk after IR, aYAP modRNA\u2013treated mice had better heart function as well as reduced scar size and hypertrophic remodeling. In cultured neonatal and adult CMs, YAP attenuated H<jats:sub>2<\/jats:sub>O<jats:sub>2<\/jats:sub>- or LPS-induced CM necrosis. TLR signaling pathway components important for innate immune responses were suppressed by YAP\/TEAD1. In summary, our findings demonstrate that aYAP modRNA treatment reduces CM necrosis, cardiac inflammation, and hypertrophic remodeling after IR stress.<\/jats:p>","DOI":"10.26508\/lsa.201900424","type":"journal-article","created":{"date-parts":[[2019,12,16]],"date-time":"2019-12-16T19:34:24Z","timestamp":1576524864000},"page":"e201900424","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":52,"title":["aYAP modRNA reduces cardiac inflammation and hypertrophy in a murine ischemia-reperfusion model"],"prefix":"10.26508","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4326-0665","authenticated-orcid":true,"given":"Jinmiao","family":"Chen","sequence":"first","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Cardiovascular Surgery and Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Qing","family":"Ma","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6469-9872","authenticated-orcid":true,"given":"Justin S","family":"King","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yan","family":"Sun","sequence":"additional","affiliation":[{"name":"Masonic Medical Research Institute, Utica, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bing","family":"Xu","sequence":"additional","affiliation":[{"name":"Masonic Medical Research Institute, Utica, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoyu","family":"Zhang","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sylvia","family":"Zohrabian","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Haipeng","family":"Guo","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Department of Critical Care Medicine, Key Laboratory of Cardiovascular Remodeling and Function Research, Chinese Ministry of Education and Chinese Ministry of Health, Qilu Hospital of Shandong University, Jinan, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wenqing","family":"Cai","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital and Dana Farber Cancer Institute, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4318-7966","authenticated-orcid":true,"given":"Gavin","family":"Li","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ivone","family":"Bruno","sequence":"additional","affiliation":[{"name":"Houston Methodist Research Institute, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John P","family":"Cooke","sequence":"additional","affiliation":[{"name":"Houston Methodist Research Institute, Houston, TX, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chunsheng","family":"Wang","sequence":"additional","affiliation":[{"name":"Department of Cardiovascular Surgery and Shanghai Institute of Cardiovascular Disease, Zhongshan Hospital, Fudan University, Shanghai, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6121-0533","authenticated-orcid":true,"given":"Maria","family":"Kontaridis","sequence":"additional","affiliation":[{"name":"Masonic Medical Research Institute, Utica, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Da-Zhi","family":"Wang","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hongbo","family":"Luo","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"William T","family":"Pu","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Harvard Stem Cell Institute, Cambridge, MA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2034-2492","authenticated-orcid":true,"given":"Zhiqiang","family":"Lin","sequence":"additional","affiliation":[{"name":"Boston Children\u2019s Hospital, Boston, MA, USA"},{"name":"Masonic Medical Research Institute, Utica, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2019,12,16]]},"reference":[{"key":"2019121611341125000_3.1.e201900424.1","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/gks1147"},{"key":"2019121611341125000_3.1.e201900424.2","doi-asserted-by":"publisher","DOI":"10.1016\/j.omtm.2018.04.003"},{"key":"2019121611341125000_3.1.e201900424.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2008.09.085"},{"key":"2019121611341125000_3.1.e201900424.4","doi-asserted-by":"publisher","DOI":"10.1038\/cddis.2011.130"},{"key":"2019121611341125000_3.1.e201900424.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.jtcvs.2003.11.036"},{"key":"2019121611341125000_3.1.e201900424.6","doi-asserted-by":"publisher","DOI":"10.3389\/fcimb.2014.00182"},{"key":"2019121611341125000_3.1.e201900424.7","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.180.9.5771"},{"key":"2019121611341125000_3.1.e201900424.8","doi-asserted-by":"publisher","DOI":"10.2147\/vhrm.s5306"},{"key":"2019121611341125000_3.1.e201900424.9","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M112.436311"},{"key":"2019121611341125000_3.1.e201900424.10","doi-asserted-by":"publisher","DOI":"10.1038\/nri3800"},{"key":"2019121611341125000_3.1.e201900424.11","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.111.243162"},{"key":"2019121611341125000_3.1.e201900424.12","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-019-08852-4"},{"key":"2019121611341125000_3.1.e201900424.13","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200524408"},{"key":"2019121611341125000_3.1.e201900424.14","doi-asserted-by":"publisher","DOI":"10.4330\/wjc.v10.i9.74"},{"key":"2019121611341125000_3.1.e201900424.15","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M111.336255"},{"key":"2019121611341125000_3.1.e201900424.16","doi-asserted-by":"publisher","DOI":"10.1152\/ajpheart.1986.251.4.H863"},{"key":"2019121611341125000_3.1.e201900424.17","doi-asserted-by":"publisher","DOI":"10.1124\/mol.105.021162"},{"key":"2019121611341125000_3.1.e201900424.18","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116302108"},{"key":"2019121611341125000_3.1.e201900424.19","doi-asserted-by":"publisher","DOI":"10.1242\/dev.102798"},{"key":"2019121611341125000_3.1.e201900424.20","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830260323"},{"key":"2019121611341125000_3.1.e201900424.21","doi-asserted-by":"publisher","DOI":"10.1097\/01.shk.0000209549.03463.91"},{"key":"2019121611341125000_3.1.e201900424.22","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-018-0007-1"},{"key":"2019121611341125000_3.1.e201900424.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.02.003"},{"key":"2019121611341125000_3.1.e201900424.24","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2005.06.008"},{"key":"2019121611341125000_3.1.e201900424.25","doi-asserted-by":"publisher","DOI":"10.1007\/978-3-319-57613-8_2"},{"key":"2019121611341125000_3.1.e201900424.26","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1406508111"},{"key":"2019121611341125000_3.1.e201900424.27","doi-asserted-by":"publisher","DOI":"10.1038\/nature24045"},{"key":"2019121611341125000_3.1.e201900424.28","doi-asserted-by":"publisher","DOI":"10.1038\/nri3151"},{"key":"2019121611341125000_3.1.e201900424.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2016.09.005"},{"key":"2019121611341125000_3.1.e201900424.30","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.303632"},{"key":"2019121611341125000_3.1.e201900424.31","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCRESAHA.115.304457"},{"key":"2019121611341125000_3.1.e201900424.32","doi-asserted-by":"publisher","DOI":"10.1161\/01.CIR.80.6.1816"},{"key":"2019121611341125000_3.1.e201900424.33","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2015.12.029"},{"key":"2019121611341125000_3.1.e201900424.34","doi-asserted-by":"publisher","DOI":"10.1172\/jci.insight.93343"},{"key":"2019121611341125000_3.1.e201900424.35","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.335"},{"key":"2019121611341125000_3.1.e201900424.36","doi-asserted-by":"publisher","DOI":"10.1136\/heartjnl-2015-307804"},{"key":"2019121611341125000_3.1.e201900424.37","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.165.10.5767"},{"key":"2019121611341125000_3.1.e201900424.38","doi-asserted-by":"publisher","DOI":"10.1172\/JCI65351"},{"key":"2019121611341125000_3.1.e201900424.39","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070885"},{"key":"2019121611341125000_3.1.e201900424.40","doi-asserted-by":"publisher","DOI":"10.1101\/cshperspect.a006049"},{"key":"2019121611341125000_3.1.e201900424.41","doi-asserted-by":"publisher","DOI":"10.1248\/bpb.28.886"},{"key":"2019121611341125000_3.1.e201900424.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.amjcard.2006.11.059"},{"key":"2019121611341125000_3.1.e201900424.43","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2010.09.011"},{"key":"2019121611341125000_3.1.e201900424.44","doi-asserted-by":"publisher","DOI":"10.1161\/CIRCULATIONAHA.108.814640"},{"key":"2019121611341125000_3.1.e201900424.45","doi-asserted-by":"publisher","DOI":"10.1172\/JCI88759"},{"key":"2019121611341125000_3.1.e201900424.46","doi-asserted-by":"publisher","DOI":"10.1177\/2048872616661691"},{"key":"2019121611341125000_3.1.e201900424.47","doi-asserted-by":"publisher","DOI":"10.1038\/nrd4278"},{"key":"2019121611341125000_3.1.e201900424.48","doi-asserted-by":"publisher","DOI":"10.3389\/fcell.2017.00050"},{"key":"2019121611341125000_3.1.e201900424.49","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"2019121611341125000_3.1.e201900424.50","doi-asserted-by":"publisher","DOI":"10.1161\/JAHA.114.001445"},{"key":"2019121611341125000_3.1.e201900424.51","doi-asserted-by":"publisher","DOI":"10.1093\/cvr\/cvs018"},{"key":"2019121611341125000_3.1.e201900424.52","doi-asserted-by":"publisher","DOI":"10.2353\/ajpath.2007.060547"},{"key":"2019121611341125000_3.1.e201900424.53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1116136109"},{"key":"2019121611341125000_3.1.e201900424.54","doi-asserted-by":"publisher","DOI":"10.1038\/ni.3744"},{"key":"2019121611341125000_3.1.e201900424.55","doi-asserted-by":"publisher","DOI":"10.1007\/s11936-016-0461-y"},{"key":"2019121611341125000_3.1.e201900424.56","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1313192110"},{"key":"2019121611341125000_3.1.e201900424.57","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2012.06.037"},{"key":"2019121611341125000_3.1.e201900424.58","doi-asserted-by":"publisher","DOI":"10.1038\/nbt.2682"},{"key":"2019121611341125000_3.1.e201900424.59","doi-asserted-by":"publisher","DOI":"10.3892\/ijmm.2012.885"},{"key":"2019121611341125000_3.1.e201900424.60","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2019.03.028"},{"key":"2019121611341125000_3.1.e201900424.61","doi-asserted-by":"publisher","DOI":"10.3389\/fcvm.2016.00040"}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.201900424","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T14:09:15Z","timestamp":1704895755000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.201900424"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2019,12,16]]},"references-count":61,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2019,12,16]]},"published-print":{"date-parts":[[2020,1]]}},"alternative-id":["10.26508\/lsa.201900424"],"URL":"https:\/\/doi.org\/10.26508\/lsa.201900424","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2019,12,16]]}}}