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Alliance"],"published-print":{"date-parts":[[2019,10]]},"abstract":"<jats:p>Inclusions of disordered protein are a characteristic feature of most neurodegenerative diseases, including Huntington\u2019s disease. Huntington\u2019s disease is caused by expansion of a polyglutamine tract in the huntingtin protein; mutant huntingtin protein (mHtt) is unstable and accumulates in large intracellular inclusions both in affected individuals and when expressed in eukaryotic cells. Using mHtt-GFP expressed in <jats:italic>Saccharomyces cerevisiae<\/jats:italic>, we find that mHtt-GFP inclusions are dynamic, mobile, gel-like structures that concentrate mHtt together with the disaggregase Hsp104. Although inclusions may associate with the vacuolar membrane, the association is reversible and we find that inclusions of mHtt in <jats:italic>S. cerevisiae<\/jats:italic> are not taken up by the vacuole or other organelles. Instead, a pulse-chase study using photoconverted mHtt-mEos2 revealed that mHtt is directly and continuously removed from the inclusion body. In addition to mobile inclusions, we also imaged and tracked the movements of small particles of mHtt-GFP and determine that they move randomly. These observations suggest that inclusions may grow through the collision and coalescence of small aggregative particles.<\/jats:p>","DOI":"10.26508\/lsa.201900489","type":"journal-article","created":{"date-parts":[[2019,9,16]],"date-time":"2019-09-16T22:34:05Z","timestamp":1568673245000},"page":"e201900489","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":54,"title":["The huntingtin inclusion is a dynamic phase-separated compartment"],"prefix":"10.26508","volume":"2","author":[{"given":"Fahmida","family":"Aktar","sequence":"first","affiliation":[{"name":"Biology Department, City University of New York, York College, Queens, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chakkapong","family":"Burudpakdee","sequence":"additional","affiliation":[{"name":"Department of Biology, Pace University, New York, NY, 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