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Alliance"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>DNA repair proteins are critical to the maintenance of genomic integrity. Specific types of genotoxic factors, including reactive oxygen species generated during normal cellular metabolism or as a result of exposure to exogenous oxidative agents, frequently leads to \u201cragged\u201d single-strand DNA breaks. The latter exhibits abnormal free DNA ends containing either a 5\u2032-hydroxyl or 3\u2032-phosphate requiring correction by the dual function enzyme, polynucleotide kinase phosphatase (PNKP), before DNA polymerase and ligation reactions can occur to seal the break. <jats:italic>Pnkp<\/jats:italic> gene deletion during early murine development leads to lethality; in contrast, the role of PNKP in adult mice is unknown. To investigate the latter, we used an inducible conditional mutagenesis approach to cause global disruption of the <jats:italic>Pnkp<\/jats:italic> gene in adult mice. This resulted in a premature aging-like phenotype, characterized by impaired growth of hair follicles, seminiferous tubules, and neural progenitor cell populations. 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