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DEH-GFP live-cell imaging showed strong GFP fluorescence throughout the life-cycle, with areas of localized expression in the cytoplasm and a circular ring pattern around the nucleus that colocalized with ER markers. \u0394<jats:italic>deh<\/jats:italic> mutants showed a small but significant reduction in oocyst size compared with WT controls from day 10 postinfection onwards, and endomitotic cell division and sporogony were completely ablated, blocking parasite transmission from mosquito to vertebrate host. Ultrastructure analysis confirmed degeneration of \u0394<jats:italic>deh<\/jats:italic> oocysts, and a complete lack of sporozoite budding. Overall, DEH is evolutionarily conserved, localizes to the ER, and plays a crucial role in sporogony.<\/jats:p>","DOI":"10.26508\/lsa.202000879","type":"journal-article","created":{"date-parts":[[2020,10,26]],"date-time":"2020-10-26T20:40:51Z","timestamp":1603744851000},"page":"e202000879","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["<i>Plasmodium<\/i> DEH is ER-localized and crucial for oocyst mitotic division during malaria transmission"],"prefix":"10.26508","volume":"3","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0418-580X","authenticated-orcid":true,"given":"David S","family":"Guttery","sequence":"first","affiliation":[{"name":"School of Life Sciences, Queens Medical Centre, University of Nottingham, Nottingham, UK"},{"name":"The Leicester Cancer Research Centre, College of Life Sciences, University of Leicester, Leicester, 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