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Alliance"],"published-print":{"date-parts":[[2022,9]]},"abstract":"<jats:p>miR-486 is a muscle-enriched microRNA, or \u201cmyomiR,\u201d that has reduced expression correlated with Duchenne muscular dystrophy (DMD). To determine the function of miR-486 in normal and dystrophin-deficient muscles and elucidate miR-486 target transcripts in skeletal muscle, we characterized <jats:italic>mir-486<\/jats:italic> knockout mice (<jats:italic>mir-486<\/jats:italic> KO). <jats:italic>mir-486<\/jats:italic> KO mice developed disrupted myofiber architecture, decreased myofiber size, decreased locomotor activity, increased cardiac fibrosis, and metabolic defects were exacerbated in <jats:italic>mir-486<\/jats:italic> KO:<jats:italic>mdx<\/jats:italic><jats:sup><jats:italic>5cv<\/jats:italic><\/jats:sup> (DKO) mice. To identify direct in vivo miR-486 muscle target transcripts, we integrated RNA sequencing and chimeric miRNA eCLIP sequencing to identify key transcripts and pathways that contribute towards <jats:italic>mir-486<\/jats:italic> KO and dystrophic disease pathologies. These targets included known and novel muscle metabolic and dystrophic structural remodeling factors of muscle and skeletal muscle contractile transcript targets. Together, our studies identify miR-486 as essential for normal muscle function, a driver of pathological remodeling in dystrophin-deficient muscle, a useful biomarker for dystrophic disease progression, and highlight the use of multiple omic platforms to identify in vivo microRNA target transcripts.<\/jats:p>","DOI":"10.26508\/lsa.202101215","type":"journal-article","created":{"date-parts":[[2022,5,5]],"date-time":"2022-05-05T16:30:15Z","timestamp":1651768215000},"page":"e202101215","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":37,"title":["miR-486 is essential for muscle function and suppresses a dystrophic transcriptome"],"prefix":"10.26508","volume":"5","author":[{"given":"Adrienne","family":"Samani","sequence":"first","affiliation":[{"name":"Department of Pediatrics, Division of Neurology at Children\u2019s of Alabama and the University of Alabama at Birmingham, Birmingham, AL, 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