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Alliance"],"published-print":{"date-parts":[[2023,4]]},"abstract":"<jats:p>\n                    FAM21 (family with sequence similarity 21) is a component of the Wiskott\u2013Aldrich syndrome protein and SCAR homologue (WASH) protein complex that mediates actin polymerization at endosomal membranes to facilitate sorting of cargo-containing vesicles out of endosomes. To study the function of FAM21 in vivo, we generated conditional knockout (cKO) mice in the C57BL\/6 background in which FAM21 was specifically knocked out of CD11c-positive dendritic cells. BMDCs from those mice displayed enlarged early endosomes, and altered cell migration and morphology relative to WT cells. FAM21-cKO cells were less competent in phagocytosis and protein antigen presentation in vitro, though peptide antigen presentation was not affected. More importantly, we identified the TLR2\/CLEC4E signaling pathway as being down-regulated in FAM21-cKO BMDCs when challenged with its specific ligand\n                    <jats:italic>Candida albicans<\/jats:italic>\n                    . Moreover, FAM21-cKO mice were more susceptible to\n                    <jats:italic>C. albicans<\/jats:italic>\n                    infection than WT mice. Reconstitution of WT BMDCs in FAM21-cKO mice rescued them from lethal\n                    <jats:italic>C. albicans<\/jats:italic>\n                    infection. Thus, our study highlights the importance of FAM21 in a host immune response against a significant pathogen.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202201414","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T13:20:19Z","timestamp":1675084819000},"page":"e202201414","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["FAM21 is critical for TLR2\/CLEC4E-mediated dendritic cell function against\n                    <i>Candida albicans<\/i>"],"prefix":"10.26508","volume":"6","author":[{"given":"Rakesh","family":"Kulkarni","sequence":"first","affiliation":[{"name":"Molecular and Cell Biology, Taiwan International Graduate Program, Academia Sinica and Graduate Institute of Life Science, National Defense Medical Center, Taipei, Taiwan"},{"id":[{"id":"https:\/\/ror.org\/047sbcx71","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Molecular Biology, Academia 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