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The mammalian homolog is found to be involved in inflammation and carcinogenesis, among other physiological functions. In this study, we used the TriCEPS-based ligand\u2013receptor methodology to identify the putative binding proteins of human STC1 (hSTC1) in the human leukemia monocytic cell line, ThP-1. LC\u2013MS\/MS analysis of peptides from shortlisted hSTC1-binding proteins detected 32 peptides that belong to IGF2\/MPRI. Surface plasmon resonance assay demonstrated that hSTC1 binds to immobilized IGF2R\/MPRI with high affinity (10\u201320 nM) and capacity (Rmax 70\u2013100%). The receptor binding data are comparable with those of (CREG) cellular repressor of E1A-stimulated gene a known ligand of IGF2R\/MPRI, with Rmax of 75\u201380% and affinity values of 1\u20132 nM. The surface plasmon resonance competitive assays showed CREG competed with hSTC1 in binding to IGF2R\/MPRI. The biological effects of hSTC1 on ThP-1 cells were demonstrated via IGF2R\/MPRI to significantly reduce secreted levels of IL-1\u03b2. This is the first study to reveal the high-affinity binding of hSTC1 to the membrane receptor IGF2R\/MPRI.<\/jats:p>","DOI":"10.26508\/lsa.202201497","type":"journal-article","created":{"date-parts":[[2022,7,7]],"date-time":"2022-07-07T14:50:12Z","timestamp":1657205412000},"page":"e202201497","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":15,"title":["Identification and characterization of a membrane receptor that binds to human STC1"],"prefix":"10.26508","volume":"5","author":[{"given":"Hin Ting","family":"Wan","sequence":"first","affiliation":[{"name":"Department of Biology, Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Hong Kong SAR, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alice HM","family":"Ng","sequence":"additional","affiliation":[{"name":"Department of Biology, Croucher Institute for Environmental Sciences, Hong Kong Baptist University, Hong Kong SAR, 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