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Alliance"],"published-print":{"date-parts":[[2023,2]]},"abstract":"<jats:p>\n                    The process of spermatogenesis\u2014when germ cells differentiate into sperm\u2014is tightly regulated, and misregulation in gene expression is likely to be involved in the physiopathology of male infertility. The testis is one of the most transcriptionally rich tissues; nevertheless, the specific gene expression changes occurring during spermatogenesis are not fully understood. To better understand gene expression during spermatogenesis, we generated germ cell\u2013specific whole transcriptome profiles by systematically comparing testicular transcriptomes from tissues in which spermatogenesis is arrested at successive steps of germ cell differentiation. In these comparisons, we found thousands of differentially expressed genes between successive germ cell types of infertility patients. We demonstrate our analyses\u2019 potential to identify novel highly germ cell\u2013specific markers (TSPY4 and LUZP4 for spermatogonia; HMGB4 for round spermatids) and identified putatively misregulated genes in male infertility (\n                    <jats:italic>RWDD2A<\/jats:italic>\n                    ,\n                    <jats:italic>CCDC183<\/jats:italic>\n                    ,\n                    <jats:italic>CNNM1<\/jats:italic>\n                    ,\n                    <jats:italic>SERF1B<\/jats:italic>\n                    ). Apart from these, we found thousands of genes showing germ cell\u2013specific isoforms (including\n                    <jats:italic>SOX15<\/jats:italic>\n                    ,\n                    <jats:italic>SPATA4<\/jats:italic>\n                    ,\n                    <jats:italic>SYCP3<\/jats:italic>\n                    ,\n                    <jats:italic>MKI67<\/jats:italic>\n                    ). Our approach and dataset can help elucidate genetic and transcriptional causes for male infertility.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202201633","type":"journal-article","created":{"date-parts":[[2022,11,29]],"date-time":"2022-11-29T12:50:58Z","timestamp":1669726258000},"page":"e202201633","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":21,"title":["Transcriptome analyses in infertile men reveal germ cell\u2013specific expression and splicing patterns"],"prefix":"10.26508","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-8257-5040","authenticated-orcid":false,"given":"Lara M","family":"Siebert-Kuss","sequence":"first","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of M\u00fcnster, M\u00fcnster, 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Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nicole","family":"Terwort","sequence":"additional","affiliation":[{"name":"Centre of Reproductive Medicine and Andrology, Institute of Reproductive and Regenerative Biology, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Margot J","family":"Wyrwoll","sequence":"additional","affiliation":[{"name":"Institute of Reproductive Genetics, University of M\u00fcnster, M\u00fcnster, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jann-Frederik","family":"Cremers","sequence":"additional","affiliation":[{"name":"Department of Clinical and Surgical Andrology, Centre of Reproductive Medicine and Andrology, University Hospital of M\u00fcnster, M\u00fcnster, 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