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Alliance"],"published-print":{"date-parts":[[2023,1]]},"abstract":"<jats:p>\n                    We recently adapted a CUT&amp;RUN protocol for genome-wide profiling of chromatin modifications in the human malaria parasite\n                    <jats:italic>Plasmodium<\/jats:italic>\n                    . Using the step-by-step protocol described below, we were able to generate high-quality profiles of multiple histone modifications using only a small fraction of the cells required for ChIP-seq. Using antibodies against two commonly profiled histone modifications, H3K4me3 and H3K9me3, we show here that CUT&amp;RUN profiling is highly reproducible and closely recapitulates previously published ChIP-seq-based abundance profiles of histone marks. Finally, we show that CUT&amp;RUN requires substantially lower sequencing coverage for accurate profiling compared with ChIP-seq.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202201778","type":"journal-article","created":{"date-parts":[[2022,11,15]],"date-time":"2022-11-15T17:10:25Z","timestamp":1668532225000},"page":"e202201778","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":10,"title":["Genome-wide profiling of histone modifications in\n                    <i>Plasmodium falciparum<\/i>\n                    using CUT&amp;RUN"],"prefix":"10.26508","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7293-5575","authenticated-orcid":true,"given":"Riward Campelo","family":"Morillo","sequence":"first","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Chantal T","family":"Harris","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA"},{"name":"Immunology and Microbial Pathogenesis Program, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kit","family":"Kennedy","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Samuel R","family":"Henning","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0089-1096","authenticated-orcid":true,"given":"Bj\u00f6rn FC","family":"Kafsack","sequence":"additional","affiliation":[{"name":"Department of Microbiology and Immunology, Weill Cornell Medicine, New York, NY, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2022,11,15]]},"reference":[{"key":"2023082309280984000_6.1.e202201778.1","doi-asserted-by":"publisher","DOI":"10.1007\/s10577-015-9509-1"},{"key":"2023082309280984000_6.1.e202201778.2","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1001223"},{"key":"2023082309280984000_6.1.e202201778.3","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btu170"},{"key":"2023082309280984000_6.1.e202201778.4","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-020-61392-6"},{"key":"2023082309280984000_6.1.e202201778.5","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-04295-5"},{"key":"2023082309280984000_6.1.e202201778.6","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-021-01045-0"},{"key":"2023082309280984000_6.1.e202201778.7","doi-asserted-by":"publisher","DOI":"10.1016\/j.chom.2018.01.008"},{"key":"2023082309280984000_6.1.e202201778.8","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-4939-3578-9_16"},{"key":"2023082309280984000_6.1.e202201778.9","doi-asserted-by":"publisher","DOI":"10.1101\/2022.01.18.476397"},{"key":"2023082309280984000_6.1.e202201778.10","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3252"},{"key":"2023082309280984000_6.1.e202201778.11","doi-asserted-by":"publisher","DOI":"10.17504\/protocols.io.zcpf2vn"},{"key":"2023082309280984000_6.1.e202201778.12","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btq351"},{"key":"2023082309280984000_6.1.e202201778.13","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp324"},{"key":"2023082309280984000_6.1.e202201778.14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btp352"},{"key":"2023082309280984000_6.1.e202201778.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2018.03.016"},{"key":"2023082309280984000_6.1.e202201778.16","doi-asserted-by":"publisher","DOI":"10.1186\/1475-2875-13-112"},{"key":"2023082309280984000_6.1.e202201778.17","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-019-49348-x"},{"key":"2023082309280984000_6.1.e202201778.18","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.46314"},{"key":"2023082309280984000_6.1.e202201778.19","unstructured":"Moll K , Kaneko A , Scherf A , Wahlgren M (2013) , 6th edn. edn Methods in Malaria Research. 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