{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T10:57:07Z","timestamp":1791457027112,"version":"4.3.4"},"reference-count":62,"publisher":"Life Science Alliance, LLC","issue":"7","license":[{"start":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T00:00:00Z","timestamp":1681776000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002347","name":"Bundesministerium f\u00fcr Bildung und Forschung","doi-asserted-by":"publisher","award":["Infect-ERA grant \u201cESCential\u201d"],"award-info":[{"award-number":["Infect-ERA grant \u201cESCential\u201d"]}],"id":[{"id":"10.13039\/501100002347","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004359","name":"Swedish Research Council","doi-asserted-by":"crossref","award":["VR 2018-05766"],"award-info":[{"award-number":["VR 2018-05766"]}],"id":[{"id":"10.13039\/501100004359","id-type":"DOI","asserted-by":"crossref"}]},{"name":"Land Hesse","award":["Pandemie Netzwerk Land Hessen"],"award-info":[{"award-number":["Pandemie Netzwerk Land Hessen"]}]},{"DOI":"10.13039\/501100010767","name":"Innovative Medicines Initiative","doi-asserted-by":"publisher","award":["101005026 (MAD-CoV-2)"],"award-info":[{"award-number":["101005026 (MAD-CoV-2)"]}],"id":[{"id":"10.13039\/501100010767","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"published-print":{"date-parts":[[2023,7]]},"abstract":"<jats:p>\n                    Viruses with an RNA genome are often the cause of zoonotic infections. In order to identify novel pro-viral host cell factors, we screened a haploid insertion\u2013mutagenized mouse embryonic cell library for clones that are resistant to Rift Valley fever virus (RVFV). This screen returned the low-density lipoprotein receptor\u2013related protein 1 (LRP1) as a top hit, a plasma membrane protein involved in a wide variety of cell activities. Inactivation of\n                    <jats:italic>LRP1<\/jats:italic>\n                    in human cells reduced RVFV RNA levels already at the attachment and entry stages of infection. Moreover, the role of LRP1 in promoting RVFV infection was dependent on physiological levels of cholesterol and on endocytosis. In the human cell line HuH-7, LRP1 also promoted early infection stages of sandfly fever Sicilian virus and La Crosse virus, but had a minor effect on late infection by vesicular stomatitis virus, whereas encephalomyocarditis virus was entirely LRP1-independent. Moreover, siRNA experiments in human Calu-3 cells demonstrated that also SARS-CoV-2 infection benefitted from LRP1. Thus, we identified LRP1 as a host factor that supports infection by a spectrum of RNA viruses.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202302005","type":"journal-article","created":{"date-parts":[[2023,4,18]],"date-time":"2023-04-18T13:55:28Z","timestamp":1681826128000},"page":"e202302005","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":36,"title":["Low-density lipoprotein receptor\u2013related protein 1 (LRP1) as an auxiliary host factor for RNA viruses"],"prefix":"10.26508","volume":"6","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-0618-5611","authenticated-orcid":true,"given":"Stephanie","family":"Devignot","sequence":"first","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tim Wai","family":"Sha","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0884-2929","authenticated-orcid":true,"given":"Thomas R","family":"Burkard","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick","family":"Schmerer","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3015-4038","authenticated-orcid":true,"given":"Astrid","family":"Hagelkruys","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2371-6055","authenticated-orcid":true,"given":"Ali","family":"Mirazimi","sequence":"additional","affiliation":[{"name":"Public Health Agency of Sweden, Solna, Sweden"},{"name":"Department of Laboratory Medicine, Karolinska Institutet, Solna, Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ulrich","family":"Elling","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8194-3777","authenticated-orcid":true,"given":"Josef M","family":"Penninger","sequence":"additional","affiliation":[{"name":"Institute of Molecular Biotechnology of the Austrian Academy of Sciences (IMBA), Vienna, Austria"},{"name":"Department of Medical Genetics, Life Sciences Institute, University of British Columbia, Vancouver, Canada"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9737-337X","authenticated-orcid":true,"given":"Friedemann","family":"Weber","sequence":"additional","affiliation":[{"name":"Institute for Virology, FB10-Veterinary Medicine, Justus-Liebig University, Giessen, Germany"},{"name":"German Centre for Infection Research (DZIF), Partner Site Giessen, Giessen, Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2023,4,18]]},"reference":[{"key":"2023082309423077000_6.7.e202302005.1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms19061780"},{"key":"2023082309423077000_6.7.e202302005.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.96.22.12766"},{"key":"2023082309423077000_6.7.e202302005.3","doi-asserted-by":"publisher","DOI":"10.3390\/v8050143"},{"key":"2023082309423077000_6.7.e202302005.4","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2013.07.005"},{"key":"2023082309423077000_6.7.e202302005.5","doi-asserted-by":"publisher","DOI":"10.1021\/acscentsci.0c01537"},{"key":"2023082309423077000_6.7.e202302005.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2113874119"},{"key":"2023082309423077000_6.7.e202302005.7","doi-asserted-by":"publisher","DOI":"10.1128\/JCM.00936-07"},{"key":"2023082309423077000_6.7.e202302005.8","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.75.3.1371-1377.2001"},{"key":"2023082309423077000_6.7.e202302005.9","unstructured":"Burkard T (2017) Retrovirus-EGT. NCBI. Available at: https:\/\/www.ncbi.nlm.nih.gov\/geo\/query\/acc.cgi?acc=GSM2227065."},{"key":"2023082309423077000_6.7.e202302005.10","doi-asserted-by":"publisher","DOI":"10.3390\/v11070596"},{"key":"2023082309423077000_6.7.e202302005.11","doi-asserted-by":"publisher","DOI":"10.4161\/viru.20573"},{"key":"2023082309423077000_6.7.e202302005.12","doi-asserted-by":"publisher","DOI":"10.1007\/s13365-021-00987-9"},{"key":"2023082309423077000_6.7.e202302005.13","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2022.869406"},{"key":"2023082309423077000_6.7.e202302005.14","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-virology-091919-065806"},{"key":"2023082309423077000_6.7.e202302005.15","doi-asserted-by":"publisher","DOI":"10.2807\/1560-7917.ES.2020.25.3.2000045"},{"key":"2023082309423077000_6.7.e202302005.16","doi-asserted-by":"publisher","DOI":"10.1038\/s41564-020-0695-z"},{"key":"2023082309423077000_6.7.e202302005.17","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01364-12"},{"key":"2023082309423077000_6.7.e202302005.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2011.10.012"},{"key":"2023082309423077000_6.7.e202302005.19","doi-asserted-by":"publisher","DOI":"10.1038\/nature24027"},{"key":"2023082309423077000_6.7.e202302005.20","doi-asserted-by":"publisher","DOI":"10.1038\/s41596-019-0169-z"},{"key":"2023082309423077000_6.7.e202302005.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.coviro.2014.01.011"},{"key":"2023082309423077000_6.7.e202302005.22","doi-asserted-by":"publisher","DOI":"10.1515\/CCLM.2011.154"},{"key":"2023082309423077000_6.7.e202302005.23","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.09.001"},{"key":"2023082309423077000_6.7.e202302005.24","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00537-16"},{"key":"2023082309423077000_6.7.e202302005.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ajpath.2013.08.029"},{"key":"2023082309423077000_6.7.e202302005.26","doi-asserted-by":"publisher","DOI":"10.1038\/icb.2016.70"},{"key":"2023082309423077000_6.7.e202302005.27","doi-asserted-by":"publisher","DOI":"10.1017\/s0950268818003096"},{"key":"2023082309423077000_6.7.e202302005.28","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.REV120.013930"},{"key":"2023082309423077000_6.7.e202302005.29","doi-asserted-by":"publisher","DOI":"10.1016\/j.biopha.2021.111667"},{"key":"2023082309423077000_6.7.e202302005.30","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00135-15"},{"key":"2023082309423077000_6.7.e202302005.31","doi-asserted-by":"publisher","DOI":"10.1007\/s10393-017-1304-3"},{"key":"2023082309423077000_6.7.e202302005.32","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00016-16"},{"key":"2023082309423077000_6.7.e202302005.33","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.01589-06"},{"key":"2023082309423077000_6.7.e202302005.34","doi-asserted-by":"publisher","DOI":"10.1186\/gb-2009-10-3-r25"},{"key":"2023082309423077000_6.7.e202302005.35","doi-asserted-by":"publisher","DOI":"10.1111\/tra.12393"},{"key":"2023082309423077000_6.7.e202302005.36","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009722"},{"key":"2023082309423077000_6.7.e202302005.37","doi-asserted-by":"publisher","DOI":"10.7554\/elife.30498"},{"key":"2023082309423077000_6.7.e202302005.38","doi-asserted-by":"publisher","DOI":"10.1006\/meth.2001.1262"},{"key":"2023082309423077000_6.7.e202302005.39","doi-asserted-by":"publisher","DOI":"10.1038\/nrmicro1890"},{"key":"2023082309423077000_6.7.e202302005.40","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1005437"},{"key":"2023082309423077000_6.7.e202302005.41","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-018-03432-4"},{"key":"2023082309423077000_6.7.e202302005.42","doi-asserted-by":"publisher","DOI":"10.1016\/j.neurobiolaging.2010.05.025"},{"key":"2023082309423077000_6.7.e202302005.43","doi-asserted-by":"publisher","DOI":"10.1177\/1040638718779112"},{"key":"2023082309423077000_6.7.e202302005.44","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.02055-15"},{"key":"2023082309423077000_6.7.e202302005.45","doi-asserted-by":"publisher","DOI":"10.3389\/fmicb.2021.631274"},{"key":"2023082309423077000_6.7.e202302005.46","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.93.23.13030"},{"key":"2023082309423077000_6.7.e202302005.47","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.3047"},{"key":"2023082309423077000_6.7.e202302005.48","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2204706119"},{"key":"2023082309423077000_6.7.e202302005.49","doi-asserted-by":"publisher","DOI":"10.1126\/science.1247005"},{"key":"2023082309423077000_6.7.e202302005.50","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03493-4"},{"key":"2023082309423077000_6.7.e202302005.51","doi-asserted-by":"publisher","DOI":"10.1128\/JVI.00752-20"},{"key":"2023082309423077000_6.7.e202302005.52","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.2215600119"},{"key":"2023082309423077000_6.7.e202302005.53","doi-asserted-by":"publisher","DOI":"10.1097\/MOL.0000000000000411"},{"key":"2023082309423077000_6.7.e202302005.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2007.10.001"},{"key":"2023082309423077000_6.7.e202302005.55","unstructured":"WHO (2021) Prioritizing Diseases for Research and Development in Emergency Contexts. https:\/\/www.who.int\/activities\/prioritizing-diseases-for-research-and-development-in-emergency-contexts"},{"key":"2023082309423077000_6.7.e202302005.56","doi-asserted-by":"publisher","DOI":"10.1126\/science.abd3629"},{"key":"2023082309423077000_6.7.e202302005.57","doi-asserted-by":"publisher","DOI":"10.1016\/j.tim.2020.05.009"},{"key":"2023082309423077000_6.7.e202302005.58","doi-asserted-by":"publisher","DOI":"10.1099\/jgv.0.001296"},{"key":"2023082309423077000_6.7.e202302005.59","doi-asserted-by":"publisher","DOI":"10.14670\/hh-11-746"},{"key":"2023082309423077000_6.7.e202302005.60","doi-asserted-by":"publisher","DOI":"10.7554\/eLife.29292"},{"key":"2023082309423077000_6.7.e202302005.61","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009079"},{"key":"2023082309423077000_6.7.e202302005.62","doi-asserted-by":"publisher","DOI":"10.1242\/jcs.250597"}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.202302005","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,10]],"date-time":"2024-01-10T09:07:24Z","timestamp":1704877644000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.202302005"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2023,4,18]]},"references-count":62,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2023,4,18]]},"published-print":{"date-parts":[[2023,7]]}},"alternative-id":["10.26508\/lsa.202302005"],"URL":"https:\/\/doi.org\/10.26508\/lsa.202302005","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2023,4,18]]}}}