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In summary, this work describes the preparation and validation of a novel reporter cell line with the potential to be used as an ultimate building block for developing unique cellular CF models by CRISPR-mediated insertion of CF-causing mutations.<\/jats:p>","DOI":"10.26508\/lsa.202302045","type":"journal-article","created":{"date-parts":[[2023,11,2]],"date-time":"2023-11-02T15:00:15Z","timestamp":1698937215000},"page":"e202302045","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["CRISPR\/Cas9 bioluminescence-based assay for monitoring CFTR trafficking to the plasma membrane"],"prefix":"10.26508","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7661-3204","authenticated-orcid":true,"given":"Martin","family":"Ondra","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04qxnmv42","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Molecular and Translational Medicine, 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