{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T23:21:11Z","timestamp":1791501671673,"version":"4.3.4"},"reference-count":76,"publisher":"Life Science Alliance, LLC","issue":"4","license":[{"start":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T00:00:00Z","timestamp":1705968000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"published-print":{"date-parts":[[2024,4]]},"abstract":"<jats:p>\n                    During the COVID-19 pandemic, RNA-seq datasets were produced to investigate the virus\u2013host relationship. However, much of these data remains underexplored. To improve the search for molecular targets and biomarkers, we performed an integrated analysis of multiple RNA-seq datasets, expanding the cohort and including patients from different countries, encompassing severe and mild COVID-19 patients. Our analysis revealed that severe COVID-19 patients exhibit overexpression of genes coding for proteins of extracellular exosomes, endomembrane system, and neutrophil granules (e.g.,\n                    <jats:italic>S100A9<\/jats:italic>\n                    ,\n                    <jats:italic>LY96<\/jats:italic>\n                    , and\n                    <jats:italic>RAB1B<\/jats:italic>\n                    ), which may play an essential role in the cellular response to infection. Concurrently, these patients exhibit down-regulation of genes encoding components of the T cell receptor complex and nucleolus, including\n                    <jats:italic>TP53<\/jats:italic>\n                    ,\n                    <jats:italic>IL2RB<\/jats:italic>\n                    , and\n                    <jats:italic>NCL<\/jats:italic>\n                    . Finally, SPI1 may emerge as a central transcriptional factor associated with the up-regulated genes, whereas TP53, MYC, and MAX were associated with the down-regulated genes during COVID-19. This study identified targets and transcriptional factors, lighting on the molecular pathophysiology of syndrome coronavirus 2 infection.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202302358","type":"journal-article","created":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T11:35:13Z","timestamp":1706009713000},"page":"e202302358","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":7,"title":["Integrated analysis of RNA-seq datasets reveals novel targets and regulators of COVID-19 severity"],"prefix":"10.26508","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-9397-7061","authenticated-orcid":true,"given":"Thais Teixeira","family":"Oliveira","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wn09761","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte","place":[", UFRN, Natal, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9019-8636","authenticated-orcid":true,"given":"J\u00falia Firme","family":"Freitas","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wn09761","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte","place":[", UFRN, Natal, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Viviane Priscila Barros","family":"de Medeiros","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wn09761","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte","place":[", UFRN, Natal, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Thiago Jesus da Silva","family":"Xavier","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wn09761","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte","place":[", UFRN, Natal, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0642-3162","authenticated-orcid":true,"given":"Lucymara Fassarella","family":"Agnez-Lima","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/04wn09761","id-type":"ROR","asserted-by":"publisher"}],"name":"Departamento de Biologia Celular e Gen\u00e9tica, Universidade Federal do Rio Grande do Norte","place":[", UFRN, Natal, Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2024,1,23]]},"reference":[{"key":"2024012308350485000_7.4.e202302358.1","doi-asserted-by":"publisher","DOI":"10.3390\/ijms24065918"},{"key":"2024012308350485000_7.4.e202302358.2","doi-asserted-by":"publisher","DOI":"10.3390\/jcm9061753"},{"key":"2024012308350485000_7.4.e202302358.3","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijid.2021.02.019"},{"key":"2024012308350485000_7.4.e202302358.4","doi-asserted-by":"publisher","DOI":"10.1126\/scitranslmed.abj7521"},{"key":"2024012308350485000_7.4.e202302358.5","doi-asserted-by":"publisher","DOI":"10.1002\/pmic.201800149"},{"key":"2024012308350485000_7.4.e202302358.6","doi-asserted-by":"publisher","DOI":"10.1016\/j.drudis.2018.01.015"},{"key":"2024012308350485000_7.4.e202302358.7","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0492-x"},{"key":"2024012308350485000_7.4.e202302358.8","doi-asserted-by":"publisher","DOI":"10.1002\/jmv.25681"},{"key":"2024012308350485000_7.4.e202302358.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2021.10.006"},{"key":"2024012308350485000_7.4.e202302358.10","doi-asserted-by":"publisher","DOI":"10.1016\/j.cellin.2022.100031"},{"key":"2024012308350485000_7.4.e202302358.11","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-26457-8"},{"key":"2024012308350485000_7.4.e202302358.12","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2022.989556"},{"key":"2024012308350485000_7.4.e202302358.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.10.030"},{"key":"2024012308350485000_7.4.e202302358.14","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/bts635"},{"key":"2024012308350485000_7.4.e202302358.15","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btw354"},{"key":"2024012308350485000_7.4.e202302358.16","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20182015"},{"key":"2024012308350485000_7.4.e202302358.17","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAC247"},{"key":"2024012308350485000_7.4.e202302358.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.10.039"},{"key":"2024012308350485000_7.4.e202302358.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.jcv.2020.104362"},{"key":"2024012308350485000_7.4.e202302358.20","doi-asserted-by":"publisher","DOI":"10.1038\/bjc.1995.354"},{"key":"2024012308350485000_7.4.e202302358.21","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-020-2286-9"},{"key":"2024012308350485000_7.4.e202302358.22","doi-asserted-by":"publisher","DOI":"10.3390\/cells11162557"},{"key":"2024012308350485000_7.4.e202302358.23","doi-asserted-by":"publisher","DOI":"10.1186\/s10020-018-0023-8"},{"key":"2024012308350485000_7.4.e202302358.24","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00459-7"},{"key":"2024012308350485000_7.4.e202302358.25","doi-asserted-by":"publisher","DOI":"10.1038\/nprot.2008.211"},{"key":"2024012308350485000_7.4.e202302358.26","doi-asserted-by":"publisher","DOI":"10.1016\/j.compbiomed.2022.105684"},{"key":"2024012308350485000_7.4.e202302358.27","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15547-2"},{"key":"2024012308350485000_7.4.e202302358.28","doi-asserted-by":"publisher","DOI":"10.1016\/j.csbj.2020.12.016"},{"key":"2024012308350485000_7.4.e202302358.29","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pcbi.1003731"},{"key":"2024012308350485000_7.4.e202302358.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.lfs.2017.07.025"},{"key":"2024012308350485000_7.4.e202302358.31","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1202626"},{"key":"2024012308350485000_7.4.e202302358.32","doi-asserted-by":"publisher","DOI":"10.1186\/s12920-021-01006-w"},{"key":"2024012308350485000_7.4.e202302358.33","doi-asserted-by":"publisher","DOI":"10.1038\/s42255-021-00425-4"},{"key":"2024012308350485000_7.4.e202302358.34","doi-asserted-by":"publisher","DOI":"10.1093\/bioinformatics\/btt656"},{"key":"2024012308350485000_7.4.e202302358.35","doi-asserted-by":"publisher","DOI":"10.1186\/S13059-014-0550-8"},{"key":"2024012308350485000_7.4.e202302358.36","doi-asserted-by":"publisher","DOI":"10.1183\/13993003.00607-2020"},{"key":"2024012308350485000_7.4.e202302358.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-021-21289-y"},{"key":"2024012308350485000_7.4.e202302358.38","doi-asserted-by":"publisher","DOI":"10.1016\/j.cytogfr.2021.10.004"},{"key":"2024012308350485000_7.4.e202302358.39","doi-asserted-by":"publisher","DOI":"10.1016\/j.antiviral.2023.105550"},{"key":"2024012308350485000_7.4.e202302358.40","doi-asserted-by":"publisher","DOI":"10.1016\/j.coi.2017.07.013"},{"key":"2024012308350485000_7.4.e202302358.41","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1205459"},{"key":"2024012308350485000_7.4.e202302358.42","doi-asserted-by":"publisher","DOI":"10.1038\/sj.onc.1208477"},{"key":"2024012308350485000_7.4.e202302358.43","doi-asserted-by":"publisher","DOI":"10.1038\/nri.2016.99"},{"key":"2024012308350485000_7.4.e202302358.44","doi-asserted-by":"publisher","DOI":"10.1016\/j.cels.2020.10.003"},{"key":"2024012308350485000_7.4.e202302358.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.devcel.2015.02.011"},{"key":"2024012308350485000_7.4.e202302358.46","doi-asserted-by":"publisher","DOI":"10.1371\/journal.ppat.1009416"},{"key":"2024012308350485000_7.4.e202302358.47","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042617-053352"},{"key":"2024012308350485000_7.4.e202302358.48","doi-asserted-by":"publisher","DOI":"10.1038\/s41598-022-15898-w"},{"key":"2024012308350485000_7.4.e202302358.49","doi-asserted-by":"publisher","DOI":"10.12688\/F1000RESEARCH.4477.2"},{"key":"2024012308350485000_7.4.e202302358.50","doi-asserted-by":"publisher","DOI":"10.3390\/cells11050847"},{"key":"2024012308350485000_7.4.e202302358.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.006"},{"key":"2024012308350485000_7.4.e202302358.52","doi-asserted-by":"publisher","DOI":"10.1101\/gr.1239303"},{"key":"2024012308350485000_7.4.e202302358.53","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.202006005"},{"key":"2024012308350485000_7.4.e202302358.54","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.08.002"},{"key":"2024012308350485000_7.4.e202302358.55","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-021-03493-4"},{"key":"2024012308350485000_7.4.e202302358.56","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506580102"},{"key":"2024012308350485000_7.4.e202302358.57","doi-asserted-by":"publisher","DOI":"10.3390\/v7122955"},{"key":"2024012308350485000_7.4.e202302358.58","doi-asserted-by":"publisher","DOI":"10.1093\/NAR\/GKAC1000"},{"key":"2024012308350485000_7.4.e202302358.59","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.01202-08"},{"key":"2024012308350485000_7.4.e202302358.60","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-020-0311-8"},{"key":"2024012308350485000_7.4.e202302358.61","doi-asserted-by":"publisher","DOI":"10.3390\/v15040824"},{"key":"2024012308350485000_7.4.e202302358.62","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2006.05.010"},{"key":"2024012308350485000_7.4.e202302358.63","doi-asserted-by":"publisher","DOI":"10.1038\/s41579-020-00468-6"},{"key":"2024012308350485000_7.4.e202302358.64","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.1585"},{"key":"2024012308350485000_7.4.e202302358.65","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2020.12.004"},{"key":"2024012308350485000_7.4.e202302358.66","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-021-00296-9"},{"key":"2024012308350485000_7.4.e202302358.67","doi-asserted-by":"publisher","DOI":"10.1101\/gr.276175.121"},{"key":"2024012308350485000_7.4.e202302358.68","doi-asserted-by":"publisher","DOI":"10.1001\/jama.2020.2648"},{"key":"2024012308350485000_7.4.e202302358.69","doi-asserted-by":"publisher","DOI":"10.1111\/sji.13090"},{"key":"2024012308350485000_7.4.e202302358.70","doi-asserted-by":"publisher","DOI":"10.1038\/s41421-022-00510-2"},{"key":"2024012308350485000_7.4.e202302358.71","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20141318"},{"key":"2024012308350485000_7.4.e202302358.72","doi-asserted-by":"publisher","DOI":"10.1016\/S2213-2600(20)30079-5"},{"key":"2024012308350485000_7.4.e202302358.73","doi-asserted-by":"publisher","DOI":"10.1002\/mco2.90"},{"key":"2024012308350485000_7.4.e202302358.74","doi-asserted-by":"publisher","DOI":"10.1038\/s41423-020-0484-x"},{"key":"2024012308350485000_7.4.e202302358.75","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(20)30566-3"},{"key":"2024012308350485000_7.4.e202302358.76","doi-asserted-by":"publisher","DOI":"10.1038\/s42003-022-03986-6"}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.202302358","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,1,23]],"date-time":"2024-01-23T11:35:18Z","timestamp":1706009718000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.202302358"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,1,23]]},"references-count":76,"journal-issue":{"issue":"4","published-online":{"date-parts":[[2024,1,23]]},"published-print":{"date-parts":[[2024,4]]}},"alternative-id":["10.26508\/lsa.202302358"],"URL":"https:\/\/doi.org\/10.26508\/lsa.202302358","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,1,23]]}}}