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In contrast, all mutations affected the formation of focal adhesions (FAs), supporting the involvement of vinculin supra-activation in this process. Our findings suggest that IpaA-induced vinculin supra-activation primarily reinforces matrix adhesion in infected cells, rather than promoting bacterial invasion. Consistently, shear stress studies pointed to a key role for IpaA-induced vinculin supra-activation in accelerating and strengthening cell-matrix adhesion.<\/jats:p>","DOI":"10.26508\/lsa.202302418","type":"journal-article","created":{"date-parts":[[2024,6,4]],"date-time":"2024-06-04T16:57:37Z","timestamp":1717520257000},"page":"e202302418","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["IpaA reveals distinct modes of vinculin activation during<i>Shigella<\/i>invasion and cell-matrix adhesion"],"prefix":"10.26508","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8483-9959","authenticated-orcid":true,"given":"Benjamin","family":"Cocom-Chan","sequence":"first","affiliation":[{"name":"Team \u201cCa2+ Signaling and Microbial Infections\u201d, I2BC, Gif-sur-Yvette, France"},{"name":"Institut National de la Sant\u00e9 et de la Recherche M\u00e9dicale U1280, Gif-sur-Yvette, France"},{"name":"Centre 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