{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,3]],"date-time":"2026-09-03T21:07:13Z","timestamp":1788469633720,"version":"build-2803163510"},"reference-count":36,"publisher":"Life Science Alliance, LLC","issue":"7","license":[{"start":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T00:00:00Z","timestamp":1715212800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Vaxinano"},{"DOI":"10.13039\/100022965","name":"Ministerio de Ciencia, Tecnolog\u00eda e Innovaci\u00f3n","doi-asserted-by":"publisher","award":["785-2017"],"award-info":[{"award-number":["785-2017"]}],"id":[{"id":"10.13039\/100022965","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"accepted":{"date-parts":[[2024,4,16]]},"published-print":{"date-parts":[[2024,7]]},"abstract":"<jats:p>\n                    Toxoplasmosis is the most prevalent parasitic zoonosis worldwide, causing ocular and neurological diseases. No vaccine has been approved for human use. We evaluated the response of peripheral blood mononuclear cells (PBMCs) to a novel construct of\n                    <jats:italic>Toxoplasma gondii<\/jats:italic>\n                    total antigen in maltodextrin nanoparticles (NP\/TE) in individuals with varying infectious statuses (uninfected, chronic asymptomatic, or ocular toxoplasmosis). We analyzed the concentration of IFN-\u03b3 after NP\/TE ex vivo stimulation using ELISA and the immunophenotypes of CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    cell populations using flow cytometry. In addition, serotyping of individuals with toxoplasmosis was performed by ELISA using GRA6-derived polypeptides. Low doses of NP\/TE stimulation (0.9 \u03bcg NP\/0.3 \u03bcg TE) achieved IFN-\u03b3\u2013specific production in previously exposed human PBMCs without significant differences in the infecting serotype. Increased IFN-\u03b3 expression in CD4\n                    <jats:sup>+<\/jats:sup>\n                    effector memory cell subsets was found in patients with ocular toxoplasmosis with NP\/TE but not with TE alone. This is the first study to show how T-cell subsets respond to ex vivo stimulation with a vaccine candidate for human toxoplasmosis, providing crucial insights for future clinical trials.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202302486","type":"journal-article","created":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T10:20:18Z","timestamp":1715250018000},"page":"e202302486","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Human T-cell activation with\n                    <i>Toxoplasma gondii<\/i>\n                    antigens loaded in maltodextrin nanoparticles"],"prefix":"10.26508","volume":"7","author":[{"given":"Laura","family":"Garc\u00eda-L\u00f3pez","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01358s213","id-type":"ROR","asserted-by":"publisher"}],"name":"GEPAMOL Group, Center for Biomedical Research CIBM, Faculty of Health Sciences, University of Quind\u00edo","place":[", Armenia, Colombia"]},{"name":"Department of Molecular and Cell Biology, University of California Merced, Merced, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-4258-000X","authenticated-orcid":true,"given":"Alejandro","family":"Zamora-V\u00e9lez","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01358s213","id-type":"ROR","asserted-by":"publisher"}],"name":"GEPAMOL Group, Center for Biomedical Research CIBM, Faculty of Health Sciences, University of Quind\u00edo","place":[", Armenia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M\u00f3nica","family":"Vargas-Montes","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01358s213","id-type":"ROR","asserted-by":"publisher"}],"name":"GEPAMOL Group, Center for Biomedical Research CIBM, Faculty of Health Sciences, University of Quind\u00edo","place":[", Armenia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Juan Camilo","family":"Sanchez-Arcila","sequence":"additional","affiliation":[{"name":"Department of Molecular and Cell Biology, University of California Merced, Merced, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fran\u00e7ois","family":"Fasquelle","sequence":"additional","affiliation":[{"name":"Vaxinano, Loos, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Didier","family":"Betbeder","sequence":"additional","affiliation":[{"name":"Vaxinano, Loos, France"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6472-3329","authenticated-orcid":true,"given":"Jorge Enrique","family":"G\u00f3mez-Mar\u00edn","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01358s213","id-type":"ROR","asserted-by":"publisher"}],"name":"GEPAMOL Group, Center for Biomedical Research CIBM, Faculty of Health Sciences, University of Quind\u00edo","place":[", Armenia, Colombia"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2024,5,9]]},"reference":[{"key":"2024050907201226000_7.7.e202302486.1","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1722202115"},{"key":"2024050907201226000_7.7.e202302486.2","doi-asserted-by":"crossref","unstructured":"Mandelbrot L , Gomez-Marin JE (2024) Protozoan diseases: Toxoplasmosis Reference Module in Biomedical Sciences. Amsterdam: Elsevier. https:\/\/linkinghub.elsevier.com\/retrieve\/pii\/B9780323999670001320","DOI":"10.1016\/B978-0-323-99967-0.00132-0"},{"key":"2024050907201226000_7.7.e202302486.3","doi-asserted-by":"publisher","DOI":"10.1586\/14760584.2013.844652"},{"key":"2024050907201226000_7.7.e202302486.4","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2023.2157818"},{"key":"2024050907201226000_7.7.e202302486.5","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.11.015"},{"key":"2024050907201226000_7.7.e202302486.6","doi-asserted-by":"publisher","DOI":"10.1017\/S003118200000144X"},{"key":"2024050907201226000_7.7.e202302486.7","doi-asserted-by":"publisher","DOI":"10.3390\/VACCINES9050413"},{"key":"2024050907201226000_7.7.e202302486.8","doi-asserted-by":"publisher","DOI":"10.1016\/j.vaccine.2010.03.028"},{"key":"2024050907201226000_7.7.e202302486.9","doi-asserted-by":"publisher","DOI":"10.1016\/J.JCONREL.2016.04.014"},{"key":"2024050907201226000_7.7.e202302486.10","doi-asserted-by":"publisher","DOI":"10.1016\/J.IJPHARM.2018.08.054"},{"key":"2024050907201226000_7.7.e202302486.11","doi-asserted-by":"publisher","DOI":"10.1146\/ANNUREV-CHEMBIOENG-080615-034446"},{"key":"2024050907201226000_7.7.e202302486.12","doi-asserted-by":"publisher","DOI":"10.1016\/J.BIOMATERIALS.2015.01.056"},{"key":"2024050907201226000_7.7.e202302486.13","doi-asserted-by":"publisher","DOI":"10.14411\/FP.2019.001"},{"key":"2024050907201226000_7.7.e202302486.14","doi-asserted-by":"publisher","DOI":"10.1002\/JAT.3247"},{"key":"2024050907201226000_7.7.e202302486.15","doi-asserted-by":"publisher","DOI":"10.1021\/acsabm.0c00521"},{"key":"2024050907201226000_7.7.e202302486.16","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2020.02183"},{"key":"2024050907201226000_7.7.e202302486.17","doi-asserted-by":"publisher","DOI":"10.2217\/FMB-2016-0146"},{"key":"2024050907201226000_7.7.e202302486.18","doi-asserted-by":"publisher","DOI":"10.1016\/j.exppara.2020.108020"},{"key":"2024050907201226000_7.7.e202302486.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.micpath.2020.104465"},{"key":"2024050907201226000_7.7.e202302486.20","doi-asserted-by":"publisher","DOI":"10.1016\/j.clnu.2017.06.005"},{"key":"2024050907201226000_7.7.e202302486.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2023.02.003"},{"key":"2024050907201226000_7.7.e202302486.22","doi-asserted-by":"publisher","DOI":"10.1080\/17469899.2023.2279178"},{"key":"2024050907201226000_7.7.e202302486.23","doi-asserted-by":"publisher","DOI":"10.1007\/s00430-014-0339-0"},{"key":"2024050907201226000_7.7.e202302486.24","doi-asserted-by":"publisher","DOI":"10.1371\/journal.pntd.0002541"},{"key":"2024050907201226000_7.7.e202302486.25","doi-asserted-by":"publisher","DOI":"10.1016\/j.ijpara.2013.09.007"},{"key":"2024050907201226000_7.7.e202302486.26","doi-asserted-by":"publisher","DOI":"10.2174\/1573395509666131126231209"},{"key":"2024050907201226000_7.7.e202302486.27","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.616309"},{"key":"2024050907201226000_7.7.e202302486.28","doi-asserted-by":"publisher","DOI":"10.1080\/09273948.2023.2217906"},{"key":"2024050907201226000_7.7.e202302486.29","doi-asserted-by":"publisher","DOI":"10.3390\/VACCINES11010158"},{"key":"2024050907201226000_7.7.e202302486.30","doi-asserted-by":"publisher","DOI":"10.1128\/IAI.01722-14"},{"key":"2024050907201226000_7.7.e202302486.31","doi-asserted-by":"publisher","DOI":"10.1080\/14760584.2024.2331065"},{"key":"2024050907201226000_7.7.e202302486.32","doi-asserted-by":"publisher","DOI":"10.3389\/fimmu.2021.612747"},{"key":"2024050907201226000_7.7.e202302486.33","doi-asserted-by":"publisher","DOI":"10.1080\/21645515.2019.1669415"},{"key":"2024050907201226000_7.7.e202302486.34","unstructured":"Kassambara A (2023) ggpubr: \u201cggplot2\u201d based publication ready plots. R package version 0.6.0 https:\/\/CRAN.R-project.org\/package=ggpubr"},{"key":"2024050907201226000_7.7.e202302486.35","unstructured":"R-project (2022) R: A Language and Environment for Statistical Computing}, Author = {{R Core Team}}, Organization = {R Foundation for Statistical Computing. https:\/\/www.r-project.org\/"},{"key":"2024050907201226000_7.7.e202302486.36","unstructured":"Nowakowski S (2024) Getting started with hclust1d. Available at: https:\/\/cran.r-project.org\/web\/packages\/hclust1d\/vignettes\/getting-started.html."}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.202302486","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,5,9]],"date-time":"2024-05-09T10:20:27Z","timestamp":1715250027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.202302486"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,5,9]]},"references-count":36,"journal-issue":{"issue":"7","published-online":{"date-parts":[[2024,5,9]]},"published-print":{"date-parts":[[2024,7]]}},"alternative-id":["10.26508\/lsa.202302486"],"URL":"https:\/\/doi.org\/10.26508\/lsa.202302486","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,5,9]]}}}