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It also demonstrated efficacy in the\n                    <jats:italic>M. marinum<\/jats:italic>\n                    \u2013zebrafish embryo infection model and\n                    <jats:italic>M. tuberculosis<\/jats:italic>\n                    -infected macrophages. Notably, BT-08 synergized with established antibiotics, including vancomycin and rifampicin. Subsequent medicinal chemistry optimization led to BT-37, a non-toxic and more potent derivative, also enhancing ethidium bromide uptake and maintaining synergy with rifampicin in infected zebrafish embryos. Mutants of\n                    <jats:italic>M. marinum<\/jats:italic>\n                    resistant to BT-37 revealed that MMAR_0407 (Rv0164) is the molecular target and that this target plays a role in the observed synergy and permeability. 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