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Alliance"],"accepted":{"date-parts":[[2024,9,26]]},"published-print":{"date-parts":[[2024,12]]},"abstract":"<jats:p>\n                    SLMAP3 is a tail-anchored membrane protein that targets subcellular organelles and is believed to regulate Hippo signaling. The global loss of SLMAP3 causes late embryonic lethality in mice, with some embryos exhibiting neural tube defects such as craniorachischisis. We show here that\n                    <jats:italic>SLMAP3<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    embryos display reduced length and increased width of neural plates, signifying arrested convergent extension. The expression of planar cell polarity (PCP) components Dvl2\/3 and the activity of the downstream targets ROCK2, cofilin, and JNK1\/2 were dysregulated in\n                    <jats:italic>SLMAP3<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    E12.5 brains. Furthermore, the cytoskeletal proteins (\u03b3-tubulin, actin, and nestin) and apical components (PKC\u03b6 and ZO-1) were mislocalized in neural tubes of\n                    <jats:italic>SLMAP3<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    embryos, with a subsequent decrease in colocalization of PCP proteins (Fzd6 and pDvl2). However, no changes in PCP or cytoskeleton proteins were found in cultured neuroepithelial cells depleted of SLMAP3, suggesting an essential requirement for SLMAP3 for these processes in vivo for neurulation. The loss of SLMAP3 had no impact on Hippo signaling in\n                    <jats:italic>SLMAP3<\/jats:italic>\n                    <jats:sup>\n                      <jats:italic>\u2212\/\u2212<\/jats:italic>\n                    <\/jats:sup>\n                    embryos, brains, and neural tubes. Proteomic analysis revealed SLMAP3 in an interactome with cytoskeletal components, including nestin, tropomyosin 4, intermediate filaments, plectin, the PCP protein SCRIB, and STRIPAK members in embryonic brains. These results reveal a crucial role of SLMAP3 in neural tube development by regulating the cytoskeleton organization and PCP pathway.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202302545","type":"journal-article","created":{"date-parts":[[2024,10,4]],"date-time":"2024-10-04T14:10:13Z","timestamp":1728051013000},"page":"e202302545","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":2,"title":["SLMAP3 is essential for neurulation through mechanisms involving cytoskeletal elements, ABP, and PCP"],"prefix":"10.26508","volume":"7","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1820-0919","authenticated-orcid":true,"given":"Taha","family":"Rehmani","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03c4mmv16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","place":[", Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4912-4456","authenticated-orcid":true,"given":"Ana Paula","family":"Dias","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03c4mmv16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","place":[", Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-6740-8463","authenticated-orcid":true,"given":"Billi Dawn","family":"Applin","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03c4mmv16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","place":[", Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maysoon","family":"Salih","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03c4mmv16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","place":[", Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0835-3081","authenticated-orcid":true,"given":"Balwant S","family":"Tuana","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/03c4mmv16","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Cellular and Molecular Medicine, Faculty of Medicine, University of Ottawa","place":[", Ottawa, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2024,10,4]]},"reference":[{"key":"2024100411100681000_7.12.e202302545.1","doi-asserted-by":"publisher","DOI":"10.1002\/wdev.71"},{"key":"2024100411100681000_7.12.e202302545.2","doi-asserted-by":"crossref","unstructured":"Wigle JT , Eisenstat DD (2023) Neural induction and regionalization Neurodevelopmental Pediatrics. 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