{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T17:54:46Z","timestamp":1785434086201,"version":"3.56.0"},"reference-count":66,"publisher":"Life Science Alliance, LLC","issue":"1","license":[{"start":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T00:00:00Z","timestamp":1730246400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100000098","name":"Stem Cell Network","doi-asserted-by":"publisher","award":["FY21\/ECI-3"],"award-info":[{"award-number":["FY21\/ECI-3"]}],"id":[{"id":"10.13039\/501100000098","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100023456","name":"Defeat Duchenne Canada","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100023456","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100000024","name":"Canadian Government | Canadian Institutes of Health Research","doi-asserted-by":"publisher","award":["159923"],"award-info":[{"award-number":["159923"]}],"id":[{"id":"10.13039\/501100000024","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Richard and Edith Strauss Canada Foundation"},{"name":"Aclon Foundation"}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"accepted":{"date-parts":[[2024,10,22]]},"published-print":{"date-parts":[[2025,1]]},"abstract":"<jats:p>\n                    Duchenne muscular dystrophy (DMD) is a lethal disease caused by mutations in the\n                    <jats:italic>DMD<\/jats:italic>\n                    gene that encodes dystrophin. Dystrophin deficiency also impacts muscle stem cells (MuSCs), resulting in impaired asymmetric stem cell division and myogenic commitment. Using MuSCs from DMD patients and the DMD mouse model\n                    <jats:italic>mdx<\/jats:italic>\n                    , we found that PTPN1 phosphatase expression is up-regulated and STAT3 phosphorylation is concomitantly down-regulated in DMD MuSCs. To restore STAT3-mediated myogenic signaling, we examined the effect of K884, a novel PTPN1\/2 inhibitor, on DMD MuSCs. Treatment with K884 enhanced STAT3 phosphorylation and promoted myogenic differentiation of DMD patient-derived MuSCs. In MuSCs from\n                    <jats:italic>mdx<\/jats:italic>\n                    mice, K884 treatment increased the number of asymmetric cell divisions, correlating with enhanced myogenic differentiation. Interestingly, the pro-myogenic effect of K884 is specific to human and murine DMD MuSCs and is absent from control MuSCs. Moreover, PTPN1\/2 loss-of-function experiments indicate that the pro-myogenic impact of K884 is mediated mainly through PTPN1. We propose that PTPN1\/2 inhibition may serve as a therapeutic strategy to restore the myogenic function of MuSCs in DMD.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202402831","type":"journal-article","created":{"date-parts":[[2024,10,30]],"date-time":"2024-10-30T15:10:19Z","timestamp":1730301019000},"page":"e202402831","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":5,"title":["PTPN1\/2 inhibition promotes muscle stem cell differentiation in Duchenne muscular dystrophy"],"prefix":"10.26508","volume":"8","author":[{"given":"Yiyang","family":"Liu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shulei","family":"Li","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]},{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Goodman Cancer Institute, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6159-6942","authenticated-orcid":false,"given":"Rebecca","family":"Robertson","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0000-0603-7400","authenticated-orcid":false,"given":"Jules A","family":"Granet","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Isabelle","family":"Aubry","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]},{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Goodman Cancer Institute, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1824-3767","authenticated-orcid":true,"given":"Romina L","family":"Filippelli","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0281-541X","authenticated-orcid":false,"given":"Michel L","family":"Tremblay","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]},{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Goodman Cancer Institute, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0589-9660","authenticated-orcid":true,"given":"Natasha C","family":"Chang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Biochemistry, Faculty of Medicine and Health Sciences, McGill University","place":[", Montr\u00e9al, Canada"]},{"id":[{"id":"https:\/\/ror.org\/01pxwe438","id-type":"ROR","asserted-by":"publisher"}],"name":"Goodman Cancer Institute, McGill University","place":[", Montr\u00e9al, Canada"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2024,10,30]]},"reference":[{"key":"2025010519551032000_8.1.e202402831.1","doi-asserted-by":"publisher","DOI":"10.1038\/mtm.2014.38"},{"key":"2025010519551032000_8.1.e202402831.2","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.95.3.763"},{"key":"2025010519551032000_8.1.e202402831.3","doi-asserted-by":"publisher","DOI":"10.1038\/s41586-023-06575-7"},{"key":"2025010519551032000_8.1.e202402831.4","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(18)30026-7"},{"key":"2025010519551032000_8.1.e202402831.5","doi-asserted-by":"publisher","DOI":"10.1002\/humu.22758"},{"key":"2025010519551032000_8.1.e202402831.6","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.13.5148"},{"key":"2025010519551032000_8.1.e202402831.7","doi-asserted-by":"publisher","DOI":"10.1128\/MCB.22.7.1998-2010.2002"},{"key":"2025010519551032000_8.1.e202402831.8","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.81.4.1189"},{"key":"2025010519551032000_8.1.e202402831.9","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70271-6"},{"key":"2025010519551032000_8.1.e202402831.10","doi-asserted-by":"publisher","DOI":"10.1016\/S1474-4422(09)70272-8"},{"key":"2025010519551032000_8.1.e202402831.11","doi-asserted-by":"publisher","DOI":"10.1016\/j.molmed.2016.04.002"},{"key":"2025010519551032000_8.1.e202402831.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2018.03.022"},{"key":"2025010519551032000_8.1.e202402831.13","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00149-1"},{"key":"2025010519551032000_8.1.e202402831.14","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.87.7.2735"},{"key":"2025010519551032000_8.1.e202402831.15","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2005.07.030"},{"key":"2025010519551032000_8.1.e202402831.16","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3990"},{"key":"2025010519551032000_8.1.e202402831.17","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5407.1544"},{"key":"2025010519551032000_8.1.e202402831.18","doi-asserted-by":"publisher","DOI":"10.1016\/S0140-6736(02)07815-7"},{"key":"2025010519551032000_8.1.e202402831.19","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(91)90035-W"},{"key":"2025010519551032000_8.1.e202402831.20","doi-asserted-by":"publisher","DOI":"10.1038\/345315a0"},{"key":"2025010519551032000_8.1.e202402831.21","doi-asserted-by":"publisher","DOI":"10.1159\/000514305"},{"key":"2025010519551032000_8.1.e202402831.22","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-0716-2409-8_7"},{"key":"2025010519551032000_8.1.e202402831.23","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.94.5.1680"},{"key":"2025010519551032000_8.1.e202402831.24","doi-asserted-by":"publisher","DOI":"10.1126\/science.2173137"},{"key":"2025010519551032000_8.1.e202402831.25","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddz044"},{"key":"2025010519551032000_8.1.e202402831.26","doi-asserted-by":"publisher","DOI":"10.1016\/0092-8674(87)90579-4"},{"key":"2025010519551032000_8.1.e202402831.27","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.07.001"},{"key":"2025010519551032000_8.1.e202402831.28","doi-asserted-by":"publisher","DOI":"10.1016\/S0925-4773(97)00097-X"},{"key":"2025010519551032000_8.1.e202402831.29","doi-asserted-by":"publisher","DOI":"10.1038\/ncomms8087"},{"key":"2025010519551032000_8.1.e202402831.30","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2007.03.044"},{"key":"2025010519551032000_8.1.e202402831.31","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2009.03.013"},{"key":"2025010519551032000_8.1.e202402831.32","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbapap.2009.09.018"},{"key":"2025010519551032000_8.1.e202402831.33","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.201010131"},{"key":"2025010519551032000_8.1.e202402831.34","doi-asserted-by":"publisher","DOI":"10.1038\/nature23270"},{"key":"2025010519551032000_8.1.e202402831.35","doi-asserted-by":"publisher","DOI":"10.3390\/cells9081780"},{"key":"2025010519551032000_8.1.e202402831.36","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.9.2.493"},{"key":"2025010519551032000_8.1.e202402831.37","doi-asserted-by":"publisher","DOI":"10.1002\/mus.23810"},{"key":"2025010519551032000_8.1.e202402831.38","doi-asserted-by":"publisher","DOI":"10.1038\/nm1736"},{"key":"2025010519551032000_8.1.e202402831.39","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-med-051113-024537"},{"key":"2025010519551032000_8.1.e202402831.40","doi-asserted-by":"publisher","DOI":"10.1007\/978-1-61779-343-1_3"},{"key":"2025010519551032000_8.1.e202402831.41","doi-asserted-by":"publisher","DOI":"10.3791\/50074"},{"key":"2025010519551032000_8.1.e202402831.42","doi-asserted-by":"publisher","DOI":"10.1101\/2023.04.17.537264"},{"key":"2025010519551032000_8.1.e202402831.43","doi-asserted-by":"publisher","DOI":"10.3389\/fmed.2024.1364778"},{"key":"2025010519551032000_8.1.e202402831.44","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.90.8.3710"},{"key":"2025010519551032000_8.1.e202402831.45","doi-asserted-by":"publisher","DOI":"10.1016\/j.cyto.2015.12.025"},{"key":"2025010519551032000_8.1.e202402831.46","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3655"},{"key":"2025010519551032000_8.1.e202402831.47","doi-asserted-by":"publisher","DOI":"10.1242\/dev.069088"},{"key":"2025010519551032000_8.1.e202402831.48","doi-asserted-by":"publisher","DOI":"10.1016\/bs.ctdb.2024.01.019"},{"key":"2025010519551032000_8.1.e202402831.49","doi-asserted-by":"publisher","DOI":"10.1038\/nmeth.2019"},{"key":"2025010519551032000_8.1.e202402831.50","doi-asserted-by":"publisher","DOI":"10.1002\/jez.1402060314"},{"key":"2025010519551032000_8.1.e202402831.51","doi-asserted-by":"publisher","DOI":"10.1016\/j.chembiol.2006.09.018"},{"key":"2025010519551032000_8.1.e202402831.52","doi-asserted-by":"publisher","DOI":"10.1038\/s41536-017-0008-1"},{"key":"2025010519551032000_8.1.e202402831.53","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0506306102"},{"key":"2025010519551032000_8.1.e202402831.54","doi-asserted-by":"publisher","DOI":"10.1038\/nm.3656"},{"key":"2025010519551032000_8.1.e202402831.55","unstructured":"Tremblay ML , Pike K-A , Perez Quintero LA (2023) Enhancing CD8+ T cells for adoptive T cell therapy by inhibiting PTPN1 (PTP1B) and PTPN2 (TC-PTP). US patent 11597739, filed August 24, 2018, and issued March 07, 2023."},{"key":"2025010519551032000_8.1.e202402831.56","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2012.05.025"},{"key":"2025010519551032000_8.1.e202402831.57","doi-asserted-by":"publisher","DOI":"10.1038\/335735a0"},{"key":"2025010519551032000_8.1.e202402831.58","doi-asserted-by":"publisher","DOI":"10.1002\/cpz1.565"},{"key":"2025010519551032000_8.1.e202402831.59","doi-asserted-by":"publisher","DOI":"10.1038\/s41582-019-0203-3"},{"key":"2025010519551032000_8.1.e202402831.60","doi-asserted-by":"publisher","DOI":"10.1096\/fj.202100207R"},{"key":"2025010519551032000_8.1.e202402831.61","doi-asserted-by":"publisher","DOI":"10.1016\/j.stem.2019.01.002"},{"key":"2025010519551032000_8.1.e202402831.62","doi-asserted-by":"publisher","DOI":"10.1084\/jem.186.5.683"},{"key":"2025010519551032000_8.1.e202402831.63","doi-asserted-by":"publisher","DOI":"10.1016\/S1534-5807(02)00148-X"},{"key":"2025010519551032000_8.1.e202402831.64","doi-asserted-by":"publisher","DOI":"10.1083\/jcb.200312007"},{"key":"2025010519551032000_8.1.e202402831.65","doi-asserted-by":"publisher","DOI":"10.1126\/science.8140422"},{"key":"2025010519551032000_8.1.e202402831.66","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2016.07.041"}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.202402831","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,1,5]],"date-time":"2025-01-05T23:00:27Z","timestamp":1736118027000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.202402831"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,10,30]]},"references-count":66,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2024,10,30]]},"published-print":{"date-parts":[[2025,1]]}},"alternative-id":["10.26508\/lsa.202402831"],"URL":"https:\/\/doi.org\/10.26508\/lsa.202402831","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2024,10,30]]}}}