{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,30]],"date-time":"2026-07-30T18:23:03Z","timestamp":1785435783388,"version":"3.56.0"},"reference-count":33,"publisher":"Life Science Alliance, LLC","issue":"11","license":[{"start":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T00:00:00Z","timestamp":1755475200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100010712","name":"Viet Nam National University Ho Chi Minh City","doi-asserted-by":"publisher","award":["562-2024-18-09"],"award-info":[{"award-number":["562-2024-18-09"]}],"id":[{"id":"10.13039\/501100010712","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"accepted":{"date-parts":[[2025,8,1]]},"published-print":{"date-parts":[[2025,11]]},"abstract":"<jats:p>\n                    Ubiquitin C-terminal hydrolase L1 (UCH-L1) is a protein in the ubiquitin\u2013proteasome system involved in numerous cellular processes and implicated in various human diseases, including cancer, neurodegenerative diseases, and metabolic syndrome. However, its roles in development remain poorly understood. In this study, we used\n                    <jats:italic>Drosophila melanogaster<\/jats:italic>\n                    as a model to investigate the function of UCH-L1. The\n                    <jats:italic>Drosophila<\/jats:italic>\n                    homolog of\n                    <jats:italic>UCH-L1<\/jats:italic>\n                    ,\n                    <jats:italic>Uch<\/jats:italic>\n                    , was specifically knocked down in the eye imaginal disk using RNA interference (RNAi). The results showed that loss of Uch function induced a rough eye phenotype characterized by abnormal ommatidia, including disorganized arrangement, variable sizes, and irregular orientations. In addition, Uch knockdown significantly affected cone cells, photoreceptor cells, and pigment cells. Remarkably, these defects were fully rescued when\n                    <jats:italic>rho-1<\/jats:italic>\n                    , a protease that releases the Spitz, the ligand of EGFR signaling, was co-knocked down. Taken together, these findings strongly demonstrated that Uch plays a crucial role in the development of various\n                    <jats:italic>Drosophila<\/jats:italic>\n                    eye cell types and functions in coordination with rhomboid-1 in the\n                    <jats:italic>Drosophila<\/jats:italic>\n                    EGFR signaling pathway.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202503246","type":"journal-article","created":{"date-parts":[[2025,8,18]],"date-time":"2025-08-18T16:55:22Z","timestamp":1755536122000},"page":"e202503246","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Critical roles of\n                    <i>Drosophila<\/i>\n                    ubiquitin carboxyl-terminal hydrolase in eye development"],"prefix":"10.26508","volume":"8","author":[{"ORCID":"https:\/\/orcid.org\/0009-0003-1677-1176","authenticated-orcid":true,"given":"Trang Thi Thuy","family":"Cao","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tuan Anh","family":"Nguyen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Minh Huy Cong","family":"Nguyen","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5825-0649","authenticated-orcid":true,"given":"Trang Thi Huyen","family":"Ngo Trang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5713-323X","authenticated-orcid":true,"given":"Thao Thi Phuong","family":"Dang","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Molecular and Environmental Biotechnology, Faculty of Biology and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]},{"name":"Vietnam National University, Ho Chi Minh City, Vietnam"},{"id":[{"id":"https:\/\/ror.org\/05jfbgm49","id-type":"ROR","asserted-by":"publisher"}],"name":"Laboratory of Molecular and Biotechnology, University of Science","place":[", Ho Chi Minh City, Vietnam"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2025,8,18]]},"reference":[{"key":"2025110308151160000_8.11.e202503246.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.bbamcr.2013.08.012"},{"key":"2025110308151160000_8.11.e202503246.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0510403103"},{"key":"2025110308151160000_8.11.e202503246.3","doi-asserted-by":"publisher","DOI":"10.1093\/hmg\/ddg211"},{"key":"2025110308151160000_8.11.e202503246.4","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1812413116"},{"key":"2025110308151160000_8.11.e202503246.5","doi-asserted-by":"publisher","DOI":"10.3390\/ijms25021303"},{"key":"2025110308151160000_8.11.e202503246.6","doi-asserted-by":"publisher","DOI":"10.1002\/bies.20852"},{"key":"2025110308151160000_8.11.e202503246.7","doi-asserted-by":"publisher","DOI":"10.2174\/0929866521666140403125959"},{"key":"2025110308151160000_8.11.e202503246.8","doi-asserted-by":"crossref","unstructured":"Thao D (2018) Drosophila model in the study role of UCH-L1. In Drosophila Melanogaster - Model for Recent Advances in Genetics and Therapeutics. Farzana Khan P (ed.). pp 193\u2013208. IntechOpen.","DOI":"10.5772\/intechopen.73578"},{"key":"2025110308151160000_8.11.e202503246.9","doi-asserted-by":"publisher","DOI":"10.1007\/s00441-012-1404-x"},{"key":"2025110308151160000_8.11.e202503246.10","doi-asserted-by":"publisher","DOI":"10.1016\/0012-1606(87)90448-9"},{"key":"2025110308151160000_8.11.e202503246.11","doi-asserted-by":"crossref","unstructured":"Pappu K , Mardon G (2002) Retinal specification and determination in Drosophila. In Drosophila Eye Development, Moses K (ed), pp 5\u201320. Springer Berlin Heidelberg.","DOI":"10.1007\/978-3-540-45398-7_2"},{"key":"2025110308151160000_8.11.e202503246.12","doi-asserted-by":"crossref","unstructured":"Charlton-Perkins M , Cook TA (2010) Chapter five - building a fly eye: Terminal differentiation events of the retina, corneal lens, and pigmented epithelia. 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