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Notably, phosphoproteomic and co-immunoprecipitation assays (co-IP) revealed that GSK3A phosphorylates viral L4-22K protein at S78 and S81 residues in a partially kinase-dependent manner. Using structure modeling, protein\u2013protein docking, and truncation assays, we mapped the interaction between GSK3A\u2019s kinase domain and the 92\u2013168 aa region of the viral L4-22K protein. In addition, GSK3A acts as a broad-spectrum proviral factor for respiratory HAdV, particularly for Species B, corresponding to a high similarity in L4-22K sequences. 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