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In this study, we investigate the impact of\n                    <jats:italic>ermB<\/jats:italic>\n                    -mediated resistance on drug binding by analyzing ribosomes from\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains with varying levels of\n                    <jats:italic>ermB<\/jats:italic>\n                    expression and activity. Using cryo-electron microscopy, we determined the high-resolution structures of solithromycin-bound ribosomes, including those with dimethylated A2058. Our structural analysis reveals the specific interactions that enable solithromycin binding despite double methylation and resistance, as corroborated by microbiological and biochemical data, suggesting that further optimization of ketolide\u2013ribosome interactions could enhance macrolide efficacy against resistant\n                    <jats:italic>S. aureus<\/jats:italic>\n                    strains.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202503325","type":"journal-article","created":{"date-parts":[[2025,6,9]],"date-time":"2025-06-09T13:45:22Z","timestamp":1749476722000},"page":"e202503325","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":3,"title":["Structural studies on ribosomes of differentially macrolide-resistant\n                    <i>Staphylococcus aureus<\/i>\n                    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