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Alliance"],"accepted":{"date-parts":[[2026,5,26]]},"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    T-cell activation requires firm arrest on APCs, a process essential for effective clonal expansion and differentiation. Although the role of co-inhibitory signals and integrin-mediated adhesion in modulating T-cell arrest is established, the contribution of co-stimulatory molecules to this process remains poorly understood. Here, we developed a quantitative \u201cscan and stop\u201d assay using engineered CHO cells as minimalistic APCs to systematically assess the influence of co-stimulatory proteins on T-cell arrest. These APCs express only selected peptide\u2013MHC complexes and co-stimulatory ligands, allowing controlled investigation of their roles in both na\u00efve and experienced CD4\n                    <jats:sup>+<\/jats:sup>\n                    and CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells. We found that CD40 selectively promotes the arrest of pre-activated CD4\n                    <jats:sup>+<\/jats:sup>\n                    T cells, whereas CD70 enhances the arrest of CD8\n                    <jats:sup>+<\/jats:sup>\n                    T cells, correlating with expression patterns of their respective receptors, CD40L and CD27. High-resolution imaging further revealed mechanical deformation of APCs during synapse formation, suggesting force generation by T cells. Altogether, our results identify CD40 and CD70 as subtype-specific regulators of T-cell arrest and reveal a novel dimension in co-stimulatory control of immune synapse formation.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202503401","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T06:21:46Z","timestamp":1779776506000},"page":"e202503401","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["A \u201cscan and stop\u201d assay identifies CD40 and CD70 as selective regulators of T-cell arrest on APCs"],"prefix":"10.26508","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-1223-0812","authenticated-orcid":true,"given":"Vincent","family":"Gloe","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/030dhdf69","id-type":"ROR","asserted-by":"publisher"}],"name":"Institute of Cell Biology and Immunology Thurgau (BITG)","place":[", University of Konstanz, 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