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Alliance"],"accepted":{"date-parts":[[2026,9,30]]},"published-print":{"date-parts":[[2027,1]]},"abstract":"<jats:p>\n                    Transcription by RNA polymerase II (Pol II) must be tightly regulated to maintain gene expression homeostasis, yet how Pol II occupancy is shaped at highly active genes remains incompletely understood. Here, we examine the relationship between the homologous recombination\u2013related factors Esc2 and Rad57 and Pol II distribution in budding yeast. Genome-wide analyses reveal that Esc2 and Rad57 preferentially associate with the gene bodies of highly transcribed housekeeping genes and are linked to a chromatin context that also includes the protein phosphatase Sit4 and its cofactor Rrd1. Loss of Esc2 and Rad57 is accompanied by increased Pol II occupancy within gene bodies, whereas the effect on steady-state RNA abundance is selective rather than uniform. Under the tested anchor-away conditions, acute Rad57 depletion in an\n                    <jats:italic>esc2<\/jats:italic>\n                    \u0394 background did not fully recapitulate the broader transcriptome imbalance and impaired growth observed after chronic Esc2\/Rad57 loss. Together, our findings reveal an association between recombination-related factors and gene-body Pol II occupancy and suggest that Esc2\/Rad57 loss is linked to transcription-associated chromatin changes in yeast.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202603673","type":"journal-article","created":{"date-parts":[[2026,9,30]],"date-time":"2026-09-30T06:21:29Z","timestamp":1790749289000},"page":"e202603673","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Esc2 and Rad57 contribute to RNA polymerase II distribution at highly transcribed genes"],"prefix":"10.26508","volume":"10","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-2449-0863","authenticated-orcid":true,"given":"Hiroshi","family":"Masumoto","sequence":"first","affiliation":[{"name":"Biomedical Research Support Center (BRSC), Nagasaki University School of Medicine, Nagasaki, Japan"},{"name":"Research Center for Advanced Genomics, Nagasaki University School of Medicine, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3118-2700","authenticated-orcid":true,"given":"Hideki","family":"Muto","sequence":"additional","affiliation":[{"name":"Biomedical Research Support Center (BRSC), Nagasaki University School of Medicine, Nagasaki, Japan"},{"name":"Research Center for Advanced Genomics, Nagasaki University School of Medicine, Nagasaki, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yohei","family":"Kurosaki","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/058h74p94","id-type":"ROR","asserted-by":"publisher"}],"name":"National Research Center for the Control and Prevention of Infectious Diseases, Nagasaki University","place":[", Nagasaki, Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5391-6595","authenticated-orcid":true,"given":"Hironori","family":"Niki","sequence":"additional","affiliation":[{"name":"Microbial Physiology Laboratory, Department of Gene Function and Phenomics, National Institute of Genetics, Shizuoka, Japan"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2026,10,12]]},"reference":[{"key":"2026101213451038000_10.1.e202603673.1","doi-asserted-by":"publisher","DOI":"10.1016\/j.molcel.2023.04.011"},{"key":"2026101213451038000_10.1.e202603673.2","doi-asserted-by":"publisher","DOI":"10.1186\/s12915-021-01030-3"},{"key":"2026101213451038000_10.1.e202603673.3","unstructured":"Amberg CD , Burke JD , Strathern NJ (2005) Methods in Yeast Genetics A Cold Spring Harbor Laboratory Course Manual. 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