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Alliance"],"accepted":{"date-parts":[[2026,8,31]]},"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:p>\n                    Skeletal muscle adapts to exercise through rapid transcriptional remodeling, but regulators that link contractile activity to these gene programs remain unclear. Here we show that MAFF, a small MAF transcription factor, is consistently induced by acute exercise in both human and mouse skeletal muscle, with induction restricted to muscles recruited by the exercise modality. In C2C12 cells,\n                    <jats:italic>Maff<\/jats:italic>\n                    was induced by in vitro electrical pulse stimulation model. Using CRISPR\/Cas9\u2013mediated\n                    <jats:italic>Maff<\/jats:italic>\n                    knockout C2C12 cells, we found that loss of\n                    <jats:italic>Maff<\/jats:italic>\n                    reduced myogenic fusion with accumulation of unfused nuclei. RNA sequencing revealed broad reprogramming in\n                    <jats:italic>Maff<\/jats:italic>\n                    -deficient myotubes, including reduced expression of muscle structural and contractile genes and increased interferon\/immune responses and extracellular matrix\u2013related signatures. Moreover,\n                    <jats:italic>Maff<\/jats:italic>\n                    deficiency attenuated a subset of electrical pulse stimulation\u2013responsive genes. Together, these data identify MAFF as a component of the transcriptional machinery supporting myogenic maturation and contraction-evoked gene regulation.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202603709","type":"journal-article","created":{"date-parts":[[2026,9,1]],"date-time":"2026-09-01T06:21:30Z","timestamp":1788243690000},"page":"e202603709","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["MAFF is a transcription factor that regulates contraction-induced skeletal muscle transcription"],"prefix":"10.26508","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0004-6286-9884","authenticated-orcid":true,"given":"Taku","family":"Fukushima","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/02h6cs343","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Physiology, School of Medicine, Aichi Medical University","place":[", Nagakute, 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