{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T16:09:15Z","timestamp":1790266155729,"version":"4.1.0"},"reference-count":45,"publisher":"Life Science Alliance, LLC","issue":"12","license":[{"start":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T00:00:00Z","timestamp":1790208000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000002","name":"National Institutes of Health","doi-asserted-by":"publisher","award":["R01AI172919"],"award-info":[{"award-number":["R01AI172919"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS National Institutes of Health","doi-asserted-by":"publisher","award":["T32GM148331"],"award-info":[{"award-number":["T32GM148331"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["T32GM135746"],"award-info":[{"award-number":["T32GM135746"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["T32GM008444"],"award-info":[{"award-number":["T32GM008444"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"HHS | National Institutes of Health","doi-asserted-by":"publisher","award":["T32AI007539"],"award-info":[{"award-number":["T32AI007539"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"accepted":{"date-parts":[[2026,9,10]]},"published-print":{"date-parts":[[2026,12]]},"abstract":"<jats:p>Much of the immunology community relies on human IL-2 to support murine CD8 T-cell expansion in vitro. Although human and murine IL-2 share high similarity in amino acid sequence and exhibit similar biological activity, the potential cross-species implications of this practice remain largely unexamined, representing a significant yet overlooked variable in many in vitro studies that serve as the foundation of T-cell biology. Here, we compared the effects of human and murine IL-2 on CD8 T cells in vitro to uncover potential differences in T-cell biology. Murine IL-2 more effectively supported the survival of mouse na\u00efve CD8 T cells in vitro and induced a distinct phenotype of IL-2 receptor expression on activated mouse CD8 T cells. In addition, murine IL-2 promoted enhanced antigen-independent expansion of memory-like CD8 T cells from na\u00efve animals. Collectively, these findings should encourage laboratories to reevaluate their human IL-2 dosing strategies or consider transitioning to murine IL-2 for in vitro studies using murine CD8 T cells.<\/jats:p>","DOI":"10.26508\/lsa.202603711","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T06:21:30Z","timestamp":1789107690000},"page":"e202603711","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":0,"title":["Murine IL-2 outperforms human IL-2 in supporting the survival of na\u00efve mouse CD8 T cells in vitro"],"prefix":"10.26508","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0007-7578-1920","authenticated-orcid":true,"given":"Yangle","family":"Yu","sequence":"first","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Tete I","family":"Obot","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Madaleine M","family":"Niznikiewicz","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Craig M","family":"Podszus","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julio S","family":"Dos-Santos","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6408-8732","authenticated-orcid":true,"given":"Elena L","family":"Pobezinskaya","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary and Animal Sciences, University of Massachusetts","place":[", Amherst, MA, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Priyadharshini","family":"Devarajan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6115-3559","authenticated-orcid":true,"given":"Leonid A","family":"Pobezinsky","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/0072zz521","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Veterinary and Animal Sciences, University of Massachusetts","place":[", Amherst, MA, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-3299-5888","authenticated-orcid":true,"given":"Brian S","family":"Sheridan","sequence":"additional","affiliation":[{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Department of Microbiology and Immunology, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]},{"id":[{"id":"https:\/\/ror.org\/05qghxh33","id-type":"ROR","asserted-by":"publisher"}],"name":"Center for Infectious Diseases, Renaissance School of Medicine, Stony Brook University","place":[", Stony Brook, NY, USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"12380","published-online":{"date-parts":[[2026,9,24]]},"reference":[{"key":"2026092408402210000_9.12.e202603711.1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1601453"},{"key":"2026092408402210000_9.12.e202603711.2","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.1604256113"},{"key":"2026092408402210000_9.12.e202603711.3","doi-asserted-by":"publisher","DOI":"10.1042\/BJ20050465"},{"key":"2026092408402210000_9.12.e202603711.4","first-page":"117","article-title":"Biologic activities of recombinant human interleukin 2 on murine lymphocytes","volume":"4","author":"Bleackley","year":"1985","journal-title":"Lymphokine Res"},{"key":"2026092408402210000_9.12.e202603711.5","doi-asserted-by":"publisher","DOI":"10.1038\/nri3156"},{"key":"2026092408402210000_9.12.e202603711.6","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302293"},{"key":"2026092408402210000_9.12.e202603711.7","doi-asserted-by":"publisher","DOI":"10.1002\/eji.1830190828"},{"key":"2026092408402210000_9.12.e202603711.8","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.7.4022"},{"key":"2026092408402210000_9.12.e202603711.9","doi-asserted-by":"publisher","DOI":"10.1016\/j.celrep.2018.10.002"},{"key":"2026092408402210000_9.12.e202603711.10","doi-asserted-by":"publisher","DOI":"10.1126\/science.adh8967"},{"key":"2026092408402210000_9.12.e202603711.11","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.62"},{"key":"2026092408402210000_9.12.e202603711.12","doi-asserted-by":"publisher","DOI":"10.1016\/j.cell.2021.02.021"},{"key":"2026092408402210000_9.12.e202603711.13","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2009.11.010"},{"key":"2026092408402210000_9.12.e202603711.14","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20070543"},{"key":"2026092408402210000_9.12.e202603711.15","doi-asserted-by":"publisher","DOI":"10.1038\/313402a0"},{"key":"2026092408402210000_9.12.e202603711.16","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(01)00167-4"},{"key":"2026092408402210000_9.12.e202603711.17","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0307298101"},{"key":"2026092408402210000_9.12.e202603711.18","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.1302985"},{"key":"2026092408402210000_9.12.e202603711.19","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2013.01.004"},{"key":"2026092408402210000_9.12.e202603711.20","doi-asserted-by":"publisher","DOI":"10.1006\/cyto.1996.0082"},{"key":"2026092408402210000_9.12.e202603711.21","doi-asserted-by":"publisher","DOI":"10.1016\/j.xpro.2023.102301"},{"key":"2026092408402210000_9.12.e202603711.22","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.immunol.26.021607.090357"},{"key":"2026092408402210000_9.12.e202603711.23","doi-asserted-by":"publisher","DOI":"10.1038\/s43018-023-00652-6"},{"key":"2026092408402210000_9.12.e202603711.24","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200113296"},{"key":"2026092408402210000_9.12.e202603711.25","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.0901330"},{"key":"2026092408402210000_9.12.e202603711.26","doi-asserted-by":"publisher","DOI":"10.1002\/eji.201546000"},{"key":"2026092408402210000_9.12.e202603711.27","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.138.6.1813"},{"key":"2026092408402210000_9.12.e202603711.28","doi-asserted-by":"publisher","DOI":"10.1002\/eji.200838426"},{"key":"2026092408402210000_9.12.e202603711.29","doi-asserted-by":"publisher","DOI":"10.3390\/cells13221838"},{"key":"2026092408402210000_9.12.e202603711.30","doi-asserted-by":"publisher","DOI":"10.1038\/369333a0"},{"key":"2026092408402210000_9.12.e202603711.31","doi-asserted-by":"publisher","DOI":"10.1073\/pnas.0909945107"},{"key":"2026092408402210000_9.12.e202603711.32","doi-asserted-by":"publisher","DOI":"10.1126\/science.283.5408.1745"},{"key":"2026092408402210000_9.12.e202603711.33","doi-asserted-by":"publisher","DOI":"10.1146\/annurev-immunol-042617-053352"},{"key":"2026092408402210000_9.12.e202603711.34","doi-asserted-by":"publisher","DOI":"10.1093\/nar\/13.5.1505"},{"key":"2026092408402210000_9.12.e202603711.35","doi-asserted-by":"publisher","DOI":"10.1016\/j.immuni.2024.02.001"},{"key":"2026092408402210000_9.12.e202603711.36","doi-asserted-by":"publisher","DOI":"10.1126\/scisignal.aan4931"},{"key":"2026092408402210000_9.12.e202603711.37","doi-asserted-by":"publisher","DOI":"10.1038\/s41577-018-0046-y"},{"key":"2026092408402210000_9.12.e202603711.38","doi-asserted-by":"publisher","DOI":"10.1038\/s41467-024-52902-5"},{"key":"2026092408402210000_9.12.e202603711.39","doi-asserted-by":"publisher","DOI":"10.1084\/jem.166.4.1055"},{"key":"2026092408402210000_9.12.e202603711.40","doi-asserted-by":"publisher","DOI":"10.1084\/jem.20212391"},{"key":"2026092408402210000_9.12.e202603711.41","doi-asserted-by":"publisher","DOI":"10.1002\/cpim.38"},{"key":"2026092408402210000_9.12.e202603711.42","doi-asserted-by":"publisher","DOI":"10.1126\/sciimmunol.ade3369"},{"key":"2026092408402210000_9.12.e202603711.43","doi-asserted-by":"publisher","DOI":"10.1038\/srep30923"},{"key":"2026092408402210000_9.12.e202603711.44","doi-asserted-by":"publisher","DOI":"10.1016\/S1074-7613(00)80564-6"},{"key":"2026092408402210000_9.12.e202603711.45","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.4.2018"}],"container-title":["Life Science Alliance"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/syndication.highwire.org\/content\/doi\/10.26508\/lsa.202603711","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,9,24]],"date-time":"2026-09-24T15:40:27Z","timestamp":1790264427000},"score":1,"resource":{"primary":{"URL":"https:\/\/www.life-science-alliance.org\/lookup\/doi\/10.26508\/lsa.202603711"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,9,24]]},"references-count":45,"journal-issue":{"issue":"12","published-online":{"date-parts":[[2026,9,24]]},"published-print":{"date-parts":[[2026,12]]}},"alternative-id":["10.26508\/lsa.202603711"],"URL":"https:\/\/doi.org\/10.26508\/lsa.202603711","relation":{},"ISSN":["2575-1077"],"issn-type":[{"value":"2575-1077","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,9,24]]}}}