{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,1]],"date-time":"2026-07-01T22:57:37Z","timestamp":1782946657276,"version":"3.54.5"},"reference-count":60,"publisher":"Life Science Alliance, LLC","issue":"8","license":[{"start":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T00:00:00Z","timestamp":1778630400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100000065","name":"HHS | NIH | National Institute of Neurological Disorders and Stroke","doi-asserted-by":"publisher","award":["RO1NS128040"],"award-info":[{"award-number":["RO1NS128040"]}],"id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000057","name":"HHS | NIH | National Institute of General Medical Sciences","doi-asserted-by":"publisher","award":["RO1GM143258, R35GM16151901"],"award-info":[{"award-number":["RO1GM143258, R35GM16151901"]}],"id":[{"id":"10.13039\/100000057","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Stanford Wu Tsai Neurosciences Institute","award":["Translate"],"award-info":[{"award-number":["Translate"]}]}],"content-domain":{"domain":["life-science-alliance.org"],"crossmark-restriction":true},"short-container-title":["Life Sci. Alliance"],"accepted":{"date-parts":[[2026,4,30]]},"published-print":{"date-parts":[[2026,8]]},"abstract":"<jats:p>\n                    Mitochondrial regulators are increasingly recognized for their influence on immune signaling within the tumor microenvironment (TME). In glioma, where immunosuppression limits therapeutic efficacy, we investigate how targeting the mitochondrial protein MIRO1 alters the TME. We combine single-nucleus RNA sequencing of murine gliomas treated in vivo with an MIRO1-binding compound and bulk RNA sequencing of human glioma resections treated with the same compound ex vivo. Cross-species transcriptomic integration reveals an MIRO1-responsive program in the TME. Among shared targets, we identify\n                    <jats:italic>PARP11\/Parp11<\/jats:italic>\n                    as a consistently up-regulated gene in glioma, which is down-regulated after MIRO1-binding compound treatment in both human and mouse gliomas. Cell\u2013cell communication analysis shows that a specific cluster of macrophages (MAC1), which exhibits robust\n                    <jats:italic>Parp11<\/jats:italic>\n                    and\n                    <jats:italic>Pdl1<\/jats:italic>\n                    (encoding PD-L1) expression, sends immunosuppressive signals to CD8\n                    <jats:sup>+<\/jats:sup>\n                    cytotoxic T cells, and may receive prostaglandin E\n                    <jats:sub>2<\/jats:sub>\n                    signals from another cluster of macrophages (MAC4). Targeting MIRO1 eliminates this cell circuitry and reduces the tumor cell population. Our study provides a transcriptomic framework for understanding mitochondria-immune crosstalk and nominates MIRO1-PARP11 as a potential effector axis of brain immune dysfunction.\n                  <\/jats:p>","DOI":"10.26508\/lsa.202603749","type":"journal-article","created":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T18:00:31Z","timestamp":1778695231000},"page":"e202603749","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":1,"title":["Cross-species transcriptomic integration reveals a MIRO1-mediated macrophage\u2013T cell axis in glioma"],"prefix":"10.26508","volume":"9","author":[{"ORCID":"https:\/\/orcid.org\/0009-0008-7836-6906","authenticated-orcid":true,"given":"Zehui","family":"Du","sequence":"first","affiliation":[{"name":"Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0007-3117-0570","authenticated-orcid":true,"given":"Menghan","family":"Li","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-7013-8150","authenticated-orcid":true,"given":"Brandon H","family":"Bergsneider","sequence":"additional","affiliation":[{"name":"Department of Neurosurgery, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andy P","family":"Tsai","sequence":"additional","affiliation":[{"name":"Department of Neurology and Neurological Sciences, Stanford University School of Medicine, Stanford, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kwang 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