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Alliance"],"published-print":{"date-parts":[[2021,9]]},"abstract":"<jats:p>CRISPR\/Cas9 is a promising technology for gene editing. To date, intracellular delivery vehicles for CRISPR\/Cas9 are limited by issues of immunogenicity, restricted packaging capacity, and low tolerance. Here, we report an alternative, nonviral delivery system for CRISPR\/Cas9 based on engineered exosomes. We show that non-autologous exosomes can encapsulate CRISPR\/Cas9 plasmid DNA via commonly available transfection reagents and can be delivered to recipient cancer cells to induce targeted gene deletion. As a proof-of-principle, we demonstrate that exosomes loaded with CRISPR\/Cas9 can target the mutant <jats:italic>Kras<\/jats:italic><jats:sup><jats:italic>G12D<\/jats:italic><\/jats:sup> oncogenic allele in pancreatic cancer cells to suppress proliferation and inhibit tumor growth in syngeneic subcutaneous and orthotopic models of pancreatic cancer. Exosomes may thus be a promising delivery platform for CRISPR\/Cas9 gene editing for targeted therapies.<\/jats:p>","DOI":"10.26508\/lsa.202000875","type":"journal-article","created":{"date-parts":[[2021,7,19]],"date-time":"2021-07-19T18:55:10Z","timestamp":1626720910000},"page":"e202000875","update-policy":"https:\/\/doi.org\/10.26508\/lsa.crossmarkpolicy","source":"Crossref","is-referenced-by-count":179,"title":["Exosome-mediated delivery of CRISPR\/Cas9 for targeting of oncogenic Kras<sup>G12D<\/sup> in pancreatic cancer"],"prefix":"10.26508","volume":"4","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4092-4991","authenticated-orcid":true,"given":"Kathleen M","family":"McAndrews","sequence":"first","affiliation":[{"name":"Department of Cancer Biology, Metastasis Research Center, University of Texas MD Anderson Cancer Center, Houston, TX, USA"}]},{"given":"Fei","family":"Xiao","sequence":"additional","affiliation":[{"name":"Department of Cancer Biology, Metastasis Research 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