{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,24]],"date-time":"2026-04-24T03:47:43Z","timestamp":1777002463061,"version":"3.51.4"},"reference-count":22,"publisher":"Pan American Health Organization","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,9,23]]},"abstract":"<p><bold>Objetivo.<\/bold> Detectar la presencia del gen ermB asociado a la resistencia a macr\u00f3lidos en cepas de Campylobacter spp. aisladas de pollos comercializados en Lima, Per\u00fa.<\/p>\n<p><bold>M\u00e9todos.<\/bold> Se analizaron 120 muestras de piel de pollo provenientes de tres mercados de los distritos de San Mart\u00edn de Porres (n = 30), Santa Anita (n = 20) e Independencia (n = 70), ubicados en la Provincia de Lima, Per\u00fa. Se realiz\u00f3 el an\u00e1lisis microbiol\u00f3gico de las muestras seg\u00fan las recomendaciones de la norma ISO 10272-1:2017. Para la confirmaci\u00f3n por reacci\u00f3n en cadena de la polimerasa (PCR, por sus siglas en ingl\u00e9s) de g\u00e9nero y especie, se utilizaron los cebadores (primers) 16-rARN y GlyA e hipO, respectivamente. Para evaluar la sensibilidad antibi\u00f3tica, se utiliz\u00f3 el agar M\u00fcller-Hinton sangre al 5% con sensidiscos de azitromicina (15 \u03bcg) y eritromicina (15 \u03bcg). La detecci\u00f3n del gen ermB en cepas con fenotipos resistentes se realiz\u00f3 mediante PCR convencional.<\/p>\n<p><bold>Resultados.<\/bold> Se obtuvo un total de 117 muestras positivas (97,5%), de las cuales 100% fueron compatibles con Campylobacter coli (prueba de hipurato negativa) y confirmadas por PCR. La evaluaci\u00f3n de resistencia antibi\u00f3tica en placa para azitromicina y eritromicina dio como resultado 100% de cepas con fenotipo de resistencia a estos macr\u00f3lidos, mientras que la PCR para la detecci\u00f3n del gen ermB indic\u00f3 un total de 62 positivas (53%), que fueron confirmadas por secuenciamiento.<\/p>\n<p><bold>Conclusiones.<\/bold> Estos resultados demuestran que las carcasas de pollo comercializadas en mercados de Lima presentan contaminaci\u00f3n por C. coli con una alta resistencia a macr\u00f3lidos, lo cual puede ser atribuido a la presencia del gen ermB.<\/p>","DOI":"10.26633\/rpsp.2020.60","type":"journal-article","created":{"date-parts":[[2020,9,17]],"date-time":"2020-09-17T22:03:08Z","timestamp":1600380188000},"page":"1","source":"Crossref","is-referenced-by-count":9,"title":["Detecci\u00f3n del gen ermB asociado a la resistencia a macr\u00f3lidos en cepas de Campylobacter aisladas de pollos comercializados en Lima, Per\u00fa"],"prefix":"10.26633","volume":"44","author":[{"given":"Diego","family":"Anampa","sequence":"first","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christian","family":"Benites","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"C\u00e9sar","family":"L\u00e1zaro","sequence":"additional","affiliation":[{"name":"clazarod@unmsm.edu.pe"},{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Juan","family":"Espinoza","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pedro","family":"Angulo","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Diego","family":"D\u00edaz","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alberto","family":"Manchego","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Rojas","sequence":"additional","affiliation":[{"name":"Facultad de Medicina Veterinaria, Universidad Nacional Mayor de San Marcos, Lima, Per"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11167","reference":[{"key":"ref1","unstructured":"Frasao B da S, Marin VA, Conte-Junior CA. Molecular detection, typing, and quantification of Campylobacter spp. in foods of animal origin. Compr Rev Food Sci Food Saf. 2017;16(4):721-34"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Sibanda N, McKenna A, Richmond A, Ricke SC, Callaway T, Stratakos AC, et al. A review of the effect of management practices on Campylobacter prevalence in poultry farms. Front Microbiol. 2018;9:2002.","DOI":"10.3389\/fmicb.2018.02002"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Esson D, Mather AE, Scanlan E, Gupta S, de Vries SPW, Bailey D, et al. Genomic variations leading to alterations in cell morphology of Campylobacter spp. Sci Rep. 2016;6:1-13.","DOI":"10.1038\/srep38303"},{"key":"ref4","unstructured":"Deza-Chiock C, Mendoza-P\u00e9rez J. Factores cr\u00edticos que no permiten el ingreso del pollo beneficiado industrialmente en el canal tradicional en Lima Metropolitana. Lima: Universidad Peruana de Ciencias Aplicadas; 2018. Disponible en: https:\/\/repositorioacademico.upc.edu.pe\/handle\/10757\/624852"},{"key":"ref5","doi-asserted-by":"crossref","unstructured":"Mehdi Y, L\u00e9tourneau-Montminy MP, Gaucher ML, Chorfi Y, Suresh G, Rouissi T, et al. Use of antibiotics in broiler production: global impacts and alternatives. Animal Nutrition. 2018;4(2):170-8.","DOI":"10.1016\/j.aninu.2018.03.002"},{"key":"ref6","doi-asserted-by":"crossref","unstructured":"Liu D, Liu W, Lv Z, Xia J, Li X, Hao Y, et al. Emerging erm(B)-mediated macrolide resistance associated with novel multidrug resistance genomic islands in Campylobacter. Antimicrob Agents Chemother. 2019;63(7):1-9.","DOI":"10.1128\/AAC.00153-19"},{"key":"ref7","doi-asserted-by":"crossref","unstructured":"Tang Y, Fang L, Xu C, Zhang Q. Antibiotic resistance trends and mechanisms in the foodborne pathogen, Campylobacter. Anim Health Res Rev. 2017;18(2):87-98.","DOI":"10.1017\/S1466252317000135"},{"key":"ref8","doi-asserted-by":"crossref","unstructured":"Florez-Cuadrado D, Ugarte-Ruiz M, Quesada A, Palomo G, Dom\u00ednguez L, Porrero MC. Description of an erm(B)-carrying Campylobacter coli isolate in Europe. J Antimicrob Chemother. 2015;71(3):841-3.","DOI":"10.1093\/jac\/dkv383"},{"key":"ref9","doi-asserted-by":"crossref","unstructured":"Zhang A, Song L, Liang H, Gu Y, Zhang C, Liu X, et al. Molecular subtyping and erythromycin resistance of Campylobacter in China. J Appl Microbiol. 2016;121(1):287-93.","DOI":"10.1111\/jam.13135"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Alfredson DA, Korolik V. Antibiotic resistance and resistance mechanisms in Campylobacter jejuni and Campylobacter coli. FEMS Microbiology Letters. 2007;277(2):123-32.","DOI":"10.1111\/j.1574-6968.2007.00935.x"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Liu D, Deng F, Gao Y, Yao H, Shen Z, Wu C, et al. Dissemination of erm(B) and its associated multidrug-resistance genomic islands in Campylobacter from 2013 to 2015. Veterinary Microbiology. 2017;204:20-4.","DOI":"10.1016\/j.vetmic.2017.02.022"},{"key":"ref12","unstructured":"Wei B, Kang M. Molecular basis of macrolide resistance in Campylobacter strains isolated from poultry in South Korea. BioMed Res Int. 2018;2018:1-9."},{"key":"ref13","unstructured":"Ministerio de Agricultura y Riego de Pe\u00fa (MINAGRI)). Pollo: comercializaci\u00f3n en Lima Metropolitana. Nota T\u00e9cnica No 1. 2018:11. Disponible en: https:\/\/www.minagri.gob.pe\/portal\/analisis-economico\/analisis-2018?download=12641:pollo-comercializacion-en-lima-metropolitana"},{"key":"ref14","unstructured":"International Organization for Standardization. ISO 10272-1: Horizontal method for detection and enumeration of Campylobacter spp. Part 1: Detection method. Microbiology of the food chain. 2017. Disponible en: https:\/\/www.iso.org\/standard\/63225.html"},{"key":"ref15","doi-asserted-by":"crossref","unstructured":"Linton D, Lawson AJ, Owen RJ, Stanley J. PCR detection, identification to species level, and fingerprinting of campylobacter jejuni and campylobacter coli direct from diarrheic samples. J Clin Microbiol. 1997;35(10):2568-72.","DOI":"10.1128\/JCM.35.10.2568-2572.1997"},{"key":"ref16","unstructured":"The European Committee on Antimicrobial Susceptibility Testing (EUCAST). Breakpoint tables for interpretation of MICs and zone diameters. 2019. Disponible en: http:\/\/www.eucast.org\/fileadmin\/src\/media\/PDFs\/EUCAST_files\/Breakpoint_tables\/v_9.0_Breakpoint_Tables.pdf"},{"key":"ref17","doi-asserted-by":"crossref","unstructured":"Sahin O, Shen Z, Zhang Q. Methods to study antimicrobial resistance in Campylobacter jejuni. En: Butcher J, Stintzi A, editors. Campylobacter jejuni: Methods and Protocols. New York, NY: Springer New York; 2017:29-42. Disponible en: https:\/\/doi.org\/10.1007\/978-1-4939-6536-6_4","DOI":"10.1007\/978-1-4939-6536-6_4"},{"key":"ref18","unstructured":"National Center for Biotechnology Information (NCBI). Disponible en: www.ncbi.nlm.nih.gov\/nucleotide\/"},{"key":"ref19","doi-asserted-by":"crossref","unstructured":"Kumar S, Stecher G, Tamura K. MEGA7: Molecular evolutionary genetics analysis version 7.0 for Bigger Datasets. Mol Biol Evol. 2016;33(7):1870-4.","DOI":"10.1093\/molbev\/msw054"},{"key":"ref20","doi-asserted-by":"crossref","unstructured":"L\u00e1zaro CA, Conte-Junior CA, Vilca MA, Lucas JR, Ramos DD, Manchego A, et al. Molecular identification of Campylobacter jejuni and Campylobacter coli isolated from small-scale poultry slaughterhouse in Lima, Peru. International Journal of Poultry Science. 2012;11(11):677-82.","DOI":"10.3923\/ijps.2012.677.682"},{"key":"ref21","unstructured":"Whitehouse CA, Zhao S, Tate H. Antimicrobial resistance in Campylobacter species: mechanisms and genomic epidemiology. Adv Appl Microbiol. 2018;103:1-47. doi: 10.1016\/bs.aambs.2018.01.00"},{"key":"ref22","doi-asserted-by":"crossref","unstructured":"Bolinger H, Kathariou S. The current state of macrolide resistance in Campylobacter spp.: Trends and impacts of resistance mechanisms. 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