{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2023,12,15]],"date-time":"2023-12-15T17:20:13Z","timestamp":1702660813669},"reference-count":13,"publisher":"Pan American Health Organization","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,10,10]]},"abstract":"<p>Cystic echinococcosis is endemic and hyperendemic in Uruguay. The objective of this study was to determine the species and genotype of <italic>Echinococcus granulosus sensu lato<\/italic> in symptomatic patients with cystic echinococcosis who underwent surgery, together with the location and stage of the cysts. The study included 13 patients aged between 6 and 57 years old. Samples of cysts from these cases were analyzed using DNA extraction, polymerase chain reaction amplification and sequencing. The results revealed the presence of <italic>E. granulosus sensu stricto<\/italic> in all cases, with 12 samples belonging to the G1 genotype and 1 to the G3 genotype, suggesting that disease persistence might be related to the dog\u2013sheep cycle. However, other intermediate hosts, such as cattle, could also be involved. Cysts were most frequently found in the liver, followed by muscle and other sites (e.g. pulmonary, vertebral, pelvic and cardiac); and stage CE1 was most frequently found, followed by CE2 and CE3b. Three cases occurred in children or adolescents, suggesting an active parasite cycle in at least some areas of the country. Since there is considerable diversity of <italic>E. granulosus sensu lato<\/italic> species and genotypes in South America, it is important to continue the present study in order to draw stronger epidemiological conclusions.<\/p>","DOI":"10.26633\/rpsp.2022.177","type":"journal-article","created":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T16:45:07Z","timestamp":1665506707000},"page":"1","source":"Crossref","is-referenced-by-count":1,"title":["First genetic characterization of human cystic echinococcosis in Uruguay"],"prefix":"10.26633","volume":"46","author":[{"given":"Elisa","family":"Figueredo","sequence":"first","affiliation":[{"name":"Departamento de Parasitolog\u00eda y Micolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vanessa","family":"Liporace","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda y Micolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gustavo","family":"Mourglia-Ettlin","sequence":"additional","affiliation":[{"name":"\u00c1rea Inmunolog\u00eda del Departamento de Biociencias, Facultad de Qu\u00edmica, Universidad de la Rep\u00fablica, Montevideo, Uruguay"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H\u00e9ctor Gabriel","family":"Avila","sequence":"additional","affiliation":[{"name":"Laboratorio Provincial de Zoonosis, Ministerio de Salud de la Provincia de San Juan, San Juan, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Daniel","family":"Da Rosa","sequence":"additional","affiliation":[{"name":"Departamento de Parasitolog\u00eda y Micolog\u00eda, Facultad de Medicina, Universidad de la Rep\u00fablica, Montevideo, Uruguay"},{"name":"danddr@gmail.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mara","family":"Rosenzvit","sequence":"additional","affiliation":[{"name":"Instituto de Investigaciones en Microbiolog\u00eda y Parasitolog\u00eda M\u00e9dica, Facultad de Medicina, Universidad de Buenos Aires, Buenos Aires, Argentina"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11167","reference":[{"key":"ref1","unstructured":"World Health Organization. Multicriteria-based ranking for risk management of food-borne parasites: report of a Joint FAO\/WHO expert meeting. Rome: Food and Agriculture Organization of the United Nations, World Health Organization; 2014. https:\/\/apps.who.int\/iris\/handle\/10665\/112672"},{"key":"ref2","doi-asserted-by":"crossref","unstructured":"Romig T, Ebi D, Wassermann M. Taxonomy and molecular epidemiology of Echinococcus granulosus sensu lato. Vet Parasitol. 2015;213:76-84.","DOI":"10.1016\/j.vetpar.2015.07.035"},{"key":"ref3","doi-asserted-by":"crossref","unstructured":"Nakao M, Yanagida T, Okamoto M, Knapp J, Nkouawa A, Sako Y, et al. State-of-the-art Echinococcus and Taenia: phylogenetic taxonomy of human-pathogenic tapeworms and its application to molecular diagnosis. Infect Genet Evol. 2010;10:444-52.","DOI":"10.1016\/j.meegid.2010.01.011"},{"key":"ref4","doi-asserted-by":"crossref","unstructured":"Kinkar L, Laurim\u00e4e T, Sharbatkhori M, Mirhendi H, Kia EB, Ponce-Gordo F, et al. 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Mem Inst Oswaldo Cruz. 2016;111:372-7.","DOI":"10.1590\/0074-02760160070"},{"key":"ref10","doi-asserted-by":"crossref","unstructured":"Bowles J, Blair D, McManus DP. Genetic variants within the genus Echinococcus identified by mitochondrial DNA sequencing. Mol Biochem Parasitol. 1992:54:165-74.","DOI":"10.1016\/0166-6851(92)90109-W"},{"key":"ref11","doi-asserted-by":"crossref","unstructured":"Rasib Q, Khan A, Ahmed H, Nizamuddin S, Asif F, Afzal MS, et al. Demographics of cystic echinococcosis patients treated surgically in Lahore, Pakistan: a single centre study from 2007\u20132018. Helminthologia. 2021; 58:162-72.","DOI":"10.2478\/helm-2021-0017"},{"key":"ref12","doi-asserted-by":"crossref","unstructured":"Bingham GM, Larrieu E, Uchiumi L, Mercapide C, Mujica G, Del Carpio M, et al. The economic impact of cystic echinococcosis in Rio Negro Province, Argentina. Am J Trop Med Hyg. 2016;94:615-25.","DOI":"10.4269\/ajtmh.15-0304"},{"key":"ref13","doi-asserted-by":"crossref","unstructured":"Pavletic CF, Larrieu E, Guarnera EA, Casas N, Irabedra P, Ferreira C, et al. Cystic echinococcosis in South America: a call for action. Rev Panam Salud Publica. 2017;41:e42.","DOI":"10.26633\/RPSP.2017.42"}],"container-title":["Revista Panamericana de Salud P\u00fablica"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/iris.paho.org\/bitstream\/handle\/10665.2\/56496\/v46e1772022.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,10,11]],"date-time":"2022-10-11T16:45:13Z","timestamp":1665506713000},"score":1,"resource":{"primary":{"URL":"https:\/\/iris.paho.org\/handle\/10665.2\/56496"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2022,10,10]]},"references-count":13,"alternative-id":["RPSP.2022.177"],"URL":"https:\/\/doi.org\/10.26633\/rpsp.2022.177","relation":{},"ISSN":["1020-4989","1680-5348"],"issn-type":[{"value":"1020-4989","type":"print"},{"value":"1680-5348","type":"electronic"}],"subject":[],"published":{"date-parts":[[2022,10,10]]}}}