{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T15:11:05Z","timestamp":1776093065619,"version":"3.50.1"},"reference-count":33,"publisher":"Pan American Health Organization","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,13]]},"abstract":"<title>ABSTRACT<\/title>\n                  <sec>\n                    <title>Objectives.<\/title>\n                    <p>To assess exposure to lymphatic filariasis (LF), other neglected tropical diseases (NTDs), and malaria, as well as seroprotection against vaccine-preventable diseases (VPDs), using a hotspot-based integrated serosurveillance approach in urban areas of Guyana.<\/p>\n                  <\/sec>\n                  <sec>\n                    <title>Methods<\/title>\n                    <p>To monitor the impact of interventions on LF transmission, one historically elevated LF hotspot \u2013 defined as a previously identified urban community with focal transmission \u2013 was selected in each coastal region (Regions III, IV, V, and X). A total of 300 individuals aged \u22656 years were surveyed in each hotspot using convenience sampling. Dried blood spots were tested at the U.S. Centers for Disease Control and Prevention using multiplex bead assay to analyze antigens from 12 pathogens: Wuchereria bancrofti and Brugia malayi (Wb123, Bm14, Bm33), Plasmodium vivax and Plasmodium falciparum (pvMSP1-19, pfMSP1-19), Strongyloides stercoralis (NIE), Taenia solium (rES33, T24H), Chlamydia trachomatis (Pgp3, CT694), Treponema pallidum (rp17, TmpA), and measles, rubella, tetanus, and diphtheria.<\/p>\n                  <\/sec>\n                  <sec>\n                    <title>Results<\/title>\n                    <p>Reactivity to Wb123 and Bm14 was consistent with previous reports of elevated LF transmission in these regions. Seropositivity to P. falciparum and P. vivax was higher among adults. Seroprotection against measles and rubella was highest among children and older adults, with immunity gaps in young adults. Most participants showed minimal seroprotection against tetanus and diphtheria. Taeniasis and cysticercosis had low seropositivity across all sites. C. trachomatis and T. pallidum antigens showed age-related increases.<\/p>\n                  <\/sec>\n                  <sec>\n                    <title>Conclusions.<\/title>\n                    <p>Hotspot-based integrated serosurveillance using multiplex bead assay provides an efficient, targeted approach to monitor NTDs, malaria, and VPDs. Findings offer valuable insights for programmatic action, although interpretation should consider the hotspot-specific context when extrapolating results.<\/p>\n                  <\/sec>","DOI":"10.26633\/rpsp.2026.29","type":"journal-article","created":{"date-parts":[[2026,4,13]],"date-time":"2026-04-13T14:22:10Z","timestamp":1776090130000},"page":"1","source":"Crossref","is-referenced-by-count":0,"title":["Hotspot-based integrated serosurveillance of neglected tropical diseases, malaria, and vaccine-preventable diseases in urban Guyana"],"prefix":"10.26633","volume":"50","author":[{"given":"Reza A.","family":"Niles-Robin","sequence":"first","affiliation":[{"name":"Ministry of Health, Georgetown, Guyana"},{"name":"nilesrobin.vcs@gmail.com"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annastacia","family":"Sampson","sequence":"additional","affiliation":[{"name":"Ministry of Health, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Olivia","family":"Valz","sequence":"additional","affiliation":[{"name":"Ministry of Health, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Oneka","family":"Scott","sequence":"additional","affiliation":[{"name":"Ministry of Health, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kim E.","family":"Dickson","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Rainier","family":"Escalada","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Desmond","family":"Nicholson","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Janice","family":"Woolford","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Georgetown, Guyana"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Maria Paz","family":"Ade","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ronaldo","family":"Carvalho Scholte","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Lucia\u00f1ez","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Pamela","family":"Bravo","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Gloria","family":"Rey-Benito","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Silvia","family":"Ibarra-Ozcariz","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ana","family":"Morice","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Martha-Idal\u00ed","family":"Saboy\u00e1-D\u00edaz","sequence":"additional","affiliation":[{"name":"Pan American Health Organization, Washington, D.C., United States of America"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"11167","reference":[{"key":"ref1","doi-asserted-by":"crossref","unstructured":"Lessler J, Azman AS, McKay HS, Moore SM. 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