{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T16:24:20Z","timestamp":1762100660366,"version":"build-2065373602"},"reference-count":57,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":3352,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2000,11]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Uptake of 5\u2010hydroxytryptamine (5\u2010HT) into platelets is an important mechanism by which low plasma concentrations are maintained, and platelet activation may therefore result in significant release of this vasoconstrictor. The present study examined the kinetics of active uptake of radiolabelled [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT by washed equine platelets\n                    <jats:italic>in vitro<\/jats:italic>\n                    , and investigated the effects on this process of 4 other naturally occurring monoamines which may be released from the caecum in conditions of carbohydrate overload. The release of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT by platelets was also studied, since platelet accumulation and activation has been associated with acute laminitis. Release of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT was measured in response to platelet activating factor (PAF), unlabelled 5\u2010HT and the indirect activation of platelets by endotoxin in the presence of blood leucocytes. K\n                    <jats:sub>m<\/jats:sub>\n                    value for the uptake of 5\u2010HT by equine platelets was 2.4 \u00b1 0.6 \u03bcmol\/l and the V\n                    <jats:sub>max<\/jats:sub>\n                    was 8.3 \u00b1 0.6 pmol [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT\/10\n                    <jats:sup>7<\/jats:sup>\n                    platelets\/min. The rate of uptake of 5 \u03bcmol\/l [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT was significantly decreased by the uptake inhibitors fluvoxamine and clomipramine. The 4 other monoamines examined all inhibited the uptake of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT in a noncompetitive manner, decreasing V\n                    <jats:sub>max<\/jats:sub>\n                    by between 17 and 82%. Incubation of platelets with LPS (0.1 mg\/ml) in the absence of leucocytes did not result in significant release of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT; however, in the presence of leucocytes 3.8 \u00b1 1.7 pmol [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT\/10\n                    <jats:sup>7<\/jats:sup>\n                    platelets (mean \u00b1 s.e.) were released. This release was significantly inhibited by parthenolide and WEB2086, but not by aspirin. This suggests that PAF from activated leucocytes was responsible for the 5\u2010HT release. These data show that 5\u2010HT uptake by equine platelets is a saturable process operating most efficiently at substrate concentrations in the low micromolar range. The noncompetitive inhibition of 5\u2010HT uptake by other naturally occurring monoamines may result in increased plasma concentrations of 5\u2010HT, as would its release by endotoxin. Such a rise in plasma 5\u2010HT concentrations may contribute to selective vasoconstriction in the equine digital circulation.\n                  <\/jats:p>","DOI":"10.2746\/042516400777584730","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"497-504","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":25,"title":["Endotoxin and dietary amines may increase plasma 5\u2010hydroxytryptamine in the horse"],"prefix":"10.1111","volume":"32","author":[{"given":"S. R.","family":"BAILEY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. M.","family":"CUNNINGHAM","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"J.","family":"ELLIOTT","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,5]]},"reference":[{"key":"e_1_2_1_2_1","unstructured":"Bailey S.R.(1998)A Study of the Vascular Effects and Factors Regulating the Concentration of 5\u2010Hydroxytryptamine in the Equine Digital Circulation.PhD Thesis University of London."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1998.tb04471.x"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(98)00520-2"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1999.tb03175.x"},{"key":"e_1_2_1_6_1","first-page":"142P","article-title":"SPICE \u2010 a simple programme for the identification of curvaceous elements","volume":"124","author":"Bailey S.R.","year":"1998","journal-title":"Br. J. Pharmacol."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1992.tb05510.x"},{"key":"e_1_2_1_8_1","first-page":"940","article-title":"Diagnosing and treating acute laminitis","volume":"91","author":"Baxter G.M.","year":"1996","journal-title":"Vet. Med."},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"508","DOI":"10.2460\/ajvr.1989.50.04.508","article-title":"In vitro reactivity of digital arteries and veins to vasoconstrictive mediators in healthy horses and in horses with early laminitis","volume":"50","author":"Baxter G.M.","year":"1989","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"crossref","first-page":"505","DOI":"10.2460\/javma.1989.195.04.505","article-title":"Evaluation of heparin for prophylaxis of equine laminitis: 71 cases (1980\u20131986)","volume":"195","author":"Belknap J.K.","year":"1989","journal-title":"J. Am. vet. med. Ass."},{"key":"e_1_2_1_11_1","first-page":"448","article-title":"Platelet function testing in the pony","volume":"38","author":"Boudreaux M.K.","year":"1988","journal-title":"Lab. anim. Sci."},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb01367.x"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1991.tb02736.x"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1977.tb07528.x"},{"key":"e_1_2_1_15_1","first-page":"347","article-title":"Insulin tolerance in laminitic ponies","volume":"47","author":"Coffman J.R.","year":"1983","journal-title":"Can. J. comp. Med."},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.2527\/jas1955.144930x"},{"key":"e_1_2_1_17_1","first-page":"130","article-title":"Serotonin and amplification mechanisms in platelet reactions","volume":"4","author":"Clerck F.F.P.","year":"1989","journal-title":"News Physiol. Sci."},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1016\/0006-2952(89)90191-3"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1989.tb05650.x"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1992.tb02815.x"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1978.tb02273.x"},{"key":"e_1_2_1_22_1","first-page":"653","article-title":"Gas liquid chromatography of amines produced by the Enterobacteriaceae","volume":"22","author":"Ghenghesh K.S.","year":"1989","journal-title":"Braz. J. Med. biol. Res."},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9673(01)92536-1"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1990.tb07057.x"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1097\/00004850-199506000-00005"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1365-2885.1994.tb00262.x"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1987.tb03420.x"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0930039"},{"key":"e_1_2_1_29_1","first-page":"146","article-title":"Heparin as a preventative for equine laminitis: a preliminary report","volume":"2","author":"Hood D.M.","year":"1982","journal-title":"Am. Ass. equine Practnrs. (Newsletter)"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1177\/000331979004100403"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1939-1676.1993.tb01012.x"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1016\/0049-3848(96)00151-X"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/0034-5288(94)90124-4"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1097\/00001721-199603000-00021"},{"key":"e_1_2_1_35_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0014-2999(96)00637-1"},{"key":"e_1_2_1_36_1","doi-asserted-by":"crossref","first-page":"7314","DOI":"10.1016\/S0021-9258(18)34378-3","article-title":"Platelet\u2010activating factor stimulates the phosphatidylinositol cycle","volume":"257","author":"Lapetina E.G.","year":"1982","journal-title":"J. Biol. Chem."},{"key":"e_1_2_1_37_1","doi-asserted-by":"publisher","DOI":"10.1016\/0007-1935(87)90024-8"},{"key":"e_1_2_1_38_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(93)90261-Z"},{"key":"e_1_2_1_39_1","doi-asserted-by":"publisher","DOI":"10.1016\/0024-3205(95)00152-V"},{"key":"e_1_2_1_40_1","first-page":"R454","article-title":"Comparative study of platelet dense granule constituents","volume":"243","author":"Meyers K.M.","year":"1982","journal-title":"Am. J. Physiol."},{"key":"e_1_2_1_41_1","doi-asserted-by":"crossref","first-page":"260","DOI":"10.2460\/ajvr.1979.40.02.260","article-title":"Characterisation of the equine platelet aggregation response","volume":"40","author":"Meyers K.M.","year":"1979","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_42_1","doi-asserted-by":"publisher","DOI":"10.1042\/cs0660443"},{"key":"e_1_2_1_43_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1992.tb09082.x"},{"key":"e_1_2_1_44_1","doi-asserted-by":"publisher","DOI":"10.1182\/blood.V50.4.555.555"},{"key":"e_1_2_1_45_1","doi-asserted-by":"crossref","first-page":"1950","DOI":"10.2460\/ajvr.1990.51.12.1950","article-title":"Evaluation of coagulation and fibrinolysis during the prodromal stages of carbohydrate\u2010induced acute laminitis in the horse","volume":"51","author":"Prasse K.W.","year":"1990","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_46_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-7158.1988.tb07010.x"},{"key":"e_1_2_1_47_1","first-page":"217","volume-title":"Pharmacology","author":"Rang H.P.","year":"1995"},{"key":"e_1_2_1_48_1","doi-asserted-by":"publisher","DOI":"10.1136\/bmj.292.6527.1037"},{"key":"e_1_2_1_49_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1987.tb02571.x"},{"key":"e_1_2_1_50_1","doi-asserted-by":"publisher","DOI":"10.1038\/191073a0"},{"key":"e_1_2_1_51_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1476-5381.1969.tb07982.x"},{"key":"e_1_2_1_52_1","doi-asserted-by":"publisher","DOI":"10.1097\/00005344-199000163-00004"},{"issue":"5","key":"e_1_2_1_53_1","first-page":"S6","article-title":"Platelet\u2010derived serotonin, the endothelium and cardiovascular disease","volume":"17","author":"Vanhoutte P.M.","year":"1991","journal-title":"J. cardiovasc. Pharmacol."},{"key":"e_1_2_1_54_1","doi-asserted-by":"crossref","first-page":"606","DOI":"10.2460\/ajvr.1994.55.05.606","article-title":"Microvascular thrombosis associated with onset of acute laminitis in ponies","volume":"55","author":"Weiss D.J.","year":"1994","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_55_1","doi-asserted-by":"crossref","first-page":"986","DOI":"10.2460\/ajvr.1995.56.08.986","article-title":"Prothrombotic events in the prodromal stages of acute laminitis in horses","volume":"56","author":"Weiss D.J.","year":"1995","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_56_1","doi-asserted-by":"crossref","first-page":"814","DOI":"10.2460\/ajvr.1998.59.07.814","article-title":"Effect of a competitive inhibitor of platelet aggregation on experimentally induced laminitis in ponies","volume":"59","author":"Weiss D.J","year":"1998","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_57_1","first-page":"325","article-title":"The pharmacology of reserpine in the horse I. Effects of reserpine and imipramine on platelet uptake of serotonin in vitro and in vivo","volume":"3","author":"White C.G.","year":"1979","journal-title":"J. equine Med. Surg."},{"key":"e_1_2_1_58_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj0800324"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2746%2F042516400777584730","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.2746\/042516400777584730","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T10:55:19Z","timestamp":1762080919000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.2746\/042516400777584730"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2000,11]]},"references-count":57,"journal-issue":{"issue":"6","published-print":{"date-parts":[[2000,11]]}},"alternative-id":["10.2746\/042516400777584730"],"URL":"https:\/\/doi.org\/10.2746\/042516400777584730","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"type":"print","value":"0425-1644"},{"type":"electronic","value":"2042-3306"}],"subject":[],"published":{"date-parts":[[2000,11]]},"assertion":[{"value":"2010-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}