{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,8]],"date-time":"2026-01-08T06:32:07Z","timestamp":1767853927131,"version":"3.49.0"},"reference-count":31,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":3657,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2000,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    Processes involved in equine carpal osteochondral injury have not been established. In other species, fibronectin appears important in chondrocyte\u2010matrix interactions, and levels are increased in osteoarthritis. This investigation aimed to (a) describe fibronectin immunoreactivity in the middle carpal joint of 2\u2010year\u2010old Thoroughbreds, (b) determine topographical variations, (c) compare strenuously trained (\n                    <jats:italic>Group 1<\/jats:italic>\n                    ) or gently exercised horses (\n                    <jats:italic>Group 2<\/jats:italic>\n                    ) and (d) describe sites with early osteoarthritis.\n                    <jats:italic>Group 1<\/jats:italic>\n                    (n = 6) underwent a 19 week high intensity treadmill training programme.\n                    <jats:italic>Group 2<\/jats:italic>\n                    (n = 6) underwent 40 min walking until euthanasia. Dorsal and palmar sites on radial, intermediate and third carpal articular surfaces were prepared. Immunohistochemistry was performed using a biotin\u2010streptavidin\/peroxidase method. Cross\u2010reactivity of rabbit antihuman fibronectin antiserum with equine fibronectin was confirmed using Western blotting. Results showed: (a) fibronectin was present primarily in pericellular and interterritorial matrix locations, (b) dorsal sites had zonal immunoreactivity compared to palmar sites, (c)\n                    <jats:italic>Group 1<\/jats:italic>\n                    dorsal radial carpal cartilage had increased superficial staining compared to\n                    <jats:italic>Group 2<\/jats:italic>\n                    and (d) fibrillated cartilage showed increased intracellular and local matrical immunoreactivity (superficial zone). These findings suggest topographical and exercise\u2010related variations in fibronectin distribution, and indicate equine fibronectin is localised at sites of cartilage degeneration and released into the matrix by chondrocytes in the local area.\n                  <\/jats:p>","DOI":"10.2746\/042516400777611982","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"47-51","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":22,"title":["Equine carpal articular cartilage fibronectin distribution associated with training, joint location and cartilage deterioration"],"prefix":"10.1111","volume":"32","author":[{"given":"Rachel C.","family":"Murray","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"H. 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