{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,3]],"date-time":"2026-04-03T16:35:55Z","timestamp":1775234155991,"version":"3.50.1"},"reference-count":30,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":3048,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2001,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    In this study, \u03b2\u2010adrenoceptors of intact equine lymphocytes were identified and subclassified by (\u2010)\u2010[\n                    <jats:sup>125<\/jats:sup>\n                    I]\u2010iodocyanopindolol (ICYP) binding. ICYP binding to intact equine lymphocytes was rapid, saturable (maximal number of binding sites 320 \u00b1 20 ICYP binding sites\/cell, n = 12) and of high affinity (K\n                    <jats:sub>D<\/jats:sub>\n                    value for ICYP 14.4 \u00b1 1.7 pmol\/l, n = 12). Binding was stereospecific as shown by the 10 times greater potency of (\u2010)\u2010propranolol to inhibit binding than its (+)\u2010isomer. \u03b2\u2010adrenoceptor agonists inhibited ICYP binding with an order of potency: (\u2010)\u2010isoprenaline &gt;(\u2010)\u2010adrenaline &gt; (\u2010)\u2010noradrenaline; the same order of potency was obtained for agonist\u2010induced stimulation of lymphocyte cyclic AMPcontent.\n                  <\/jats:p>\n                  <jats:p>\n                    The selective \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    \u2010adrenoceptor antagonist ICI 118,551 was about 1000 times more potent in inhibiting ICYP binding than was the \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010selective adrenoceptor antagonist CGP 20712A. It is, therefore, concluded that in intact equine lymphocytes, ICYP labels a class of functional \u03b2\u2010adrenoceptors that belong predominantly (&gt;90%) to the \u03b2\n                    <jats:sub>2<\/jats:sub>\n                    \u2010adrenoceptor subtype; a small (&lt;10%) \u03b2\n                    <jats:sub>1<\/jats:sub>\n                    \u2010adrenoceptor component, however, cannot be ruled out completely. ICYP binding to equine lymphocytes might be a suitable model to study function and regulation of the \u03b2\u2010adrenoceptor system in the horse\n                    <jats:italic>in vivo.<\/jats:italic>\n                    The aim of this study was to characterise the \u03b2\u2010adrenoreceptor subtypes present on equine lymphocytes.\n                  <\/jats:p>","DOI":"10.2746\/042516401776254862","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"487-493","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":7,"title":["Identification and characterisation of \u03b2\u2010adrenoceptors on intact equine peripheral blood lymphocytes with the radioligand (\u2010)\u2010[125I]\u2010iodocyanopindolol"],"prefix":"10.1111","volume":"33","author":[{"given":"G.","family":"ABRAHAM","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"O.\u2010E.","family":"BRODDE","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. 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