{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:02:43Z","timestamp":1770217363625,"version":"3.49.0"},"reference-count":34,"publisher":"Wiley","issue":"6","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":2987,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2001,11]]},"abstract":"Summary<\/jats:title>\n \n Growth factors are important in healing and restoration of injured gastrointestinal tissues and, therefore, we characterised temporally the distribution and density of epidermal growth factor receptor (EGFr) in normal and peptic\u2010injured gastric squamous epithelium of horses. Lesions were induced in the equine gastric squamous epithelium using a feed deprivation protocol that results in prolonged increased gastric acidity. Fifteen mature horses, 9 geldings and 6 mares, age 3 to 20 years, were used and divided into 3 groups:\n Group 1<\/jats:italic>\n (n = 5) were subjected to euthanasia for problems unrelated to the gastrointestinal tract and had normal\u2010appearing gastric squamous mucosal epithelium;\n Groups 2<\/jats:italic>\n (n = 5) and\n 3<\/jats:italic>\n (n = 5) had lesions induced in the gastric squamous epithelium by alternating 24 h periods of feed deprivation and\n ad libitum<\/jats:italic>\n access to hay, for a total of 48 h and 96 h, respectively. Following lethal injection of a barbiturate, stomachs were removed and fixed by filling with 4\u2013 6 l 10% buffered formalin.\n <\/jats:p>\n \n Sections were made from normal stomachs and lesions in the gastric squamous epithelium adjacent to the\n margo plicatus<\/jats:italic>\n along the right side of the stomach\/greatercurvature and the lesser curvature. A modified avidin\u2010biotin immunoperoxidase technique was used to stain the formalinfixed tissue specimens for EGFr. A computerised image analysis system was used to measure area occupied by EGFr (EGFr area) and mean EGFr density in 4 zones within the epithelium extending from the basal cell layers toward the lumen. Measurements were made of epithelium in an erosion bed, at the margin of an ulcer or erosion, and 10\u201315 mm distant from the lesion margin. Additionally, EGFr area and density were measured in epithelial cells adjacent to capillaries in the epithelium.\n <\/jats:p>\n \n Intermittent feed deprivation resulted in erosion and ulceration of the gastric squamous epithelium of each horse. Mean EGFr area and density were greatest (P<0.05) in the basal layer of epithelia from all horses, and EGFr staining diminished progressively toward the lumen. Tissues from\n Group 3<\/jats:italic>\n had significantly greater EGFr area in the lesion margin than epithelia from\n Group 2.<\/jats:italic>\n EGFr density was less in the epithelia of erosion beds from\n Groups 2<\/jats:italic>\n and\n 3<\/jats:italic>\n compared to normal epithelium, and EGFr area in\n Group 2<\/jats:italic>\n erosion bed epithelia was significantly less than in normal epithelium and epithelia of\n Group 3.<\/jats:italic>\n EGFr area in cells adjacent to epithelial capillaries of\n Group 3<\/jats:italic>\n was significantly greater than that of\n Group 1.<\/jats:italic>\n Mitotic cell activity was significantly greater in epithelia associated with ulcers and erosions in\n Groups 2<\/jats:italic>\n and\n 3<\/jats:italic>\n compared to normal tissues from\n Group 1<\/jats:italic>\n horses. Staining for EGFr in the glandular mucosa adjacent to squamous epithelium at the\n margo plicatus<\/jats:italic>\n was inconsistent and typically faint when present.\n <\/jats:p>\n \n EGFr distribution in equine gastric squamous epithelium was greatest in regions of greatest cell proliferation, and these areas were in the basal layers of epithelium and immediately adjacent to capillaries. There was evidence that EGFr is induced in peptic\u2010injured equine gastric squamous epithelium. A receptor ligand, EGF or transforming growth factor\n \u03b1<\/jats:sub>\n , may be a factor in healing of gastric squamous mucosal ulcers in horses. Further research should be directed at identifying this ligand and determining its origin in equine gastric mucosa.\n <\/jats:p>","DOI":"10.2746\/042516401776563481","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"562-569","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":11,"title":["Distribution of epidermal growth factor receptor (EGFr) in normal and acute peptic\u2010injured equine gastric squamous epithelium"],"prefix":"10.1111","volume":"33","author":[{"given":"S. C.","family":"JEFFREY","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M. J.","family":"MURRAY","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E. 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