{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,7]],"date-time":"2026-02-07T02:38:59Z","timestamp":1770431939033,"version":"3.49.0"},"reference-count":38,"publisher":"Wiley","issue":"2","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":2502,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2003,3]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Reasons for performing study<\/jats:italic>\n                    : 5\u2010hydroxytryptamine (5\u2010HT; serotonin) is a potent vasoconstrictor of equine digital blood vessels and has been implicated in the pathogenesis of acute laminitis.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Objectives<\/jats:italic>\n                    : The aims of this study were firstly to examine whether cells of the digital blood vessel wall exhibited an active uptake mechanism for 5\u2010HT and to characterise its efficiency; and secondly, to study the potential inhibitory effect on this process of other amines, produced in the equine caecum.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    : Confluent monolayers of equine digital vein endothelial cells (EDVEC) and equine digital vein smooth muscle cells (EDVSMC) were incubated with [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT (0.1\u2013250 \u03bcmol\/l) and the total and active uptake calculated. Equine pulmonary vein endothelial cells (EPVEC) were used as a positive control.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    : Both EDVEC and EDVSMC showed uptake of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT by nonfaci litated diffusion; however, only EDVEC showed evidence of saturable facilitated uptake mechanism, with a K\n                    <jats:sub>m<\/jats:sub>\n                    of 41.6 \u00b1 9.3 \u03bcmol\/l, which was significantly higher than that of EPVEC (9.9 \u00b1 2.1 \u03bcmol\/l). All 6 caecally\u2010derived amines examined (tyramine, spermine, isoamylamine, tryptamine, phenylethylamine and isobutylamine) inhibited the total uptake of [\n                    <jats:sup>3<\/jats:sup>\n                    H]5\u2010HT in a concentration\u2010dependent manner, tyramine having the lowest IC\n                    <jats:sub>50<\/jats:sub>\n                    (3.7 \u00d7 10\n                    <jats:sup>\u22126<\/jats:sup>\n                    mol\/l).\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    : These data suggest that facilitated uptake into the endothelium could play a role in moderating the vasoconstrictor response to 5\u2010HT in the equine digital circulation.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Potential clinical relevance<\/jats:italic>\n                    : The vasoconstrictor action of 5\u2010HT could be potentiated by gut\u2010derived amines, providing a feasible link between GI disturbances and the pathophysiology of laminits.\n                  <\/jats:p>","DOI":"10.2746\/042516403776114171","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"164-169","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":24,"title":["Uptake of 5\u2010hydroxytryptamine by equine digital vein endothelial cells: inhibition by amines found in the equine caecum"],"prefix":"10.1111","volume":"35","author":[{"given":"S. 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