{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,4]],"date-time":"2026-02-04T15:02:09Z","timestamp":1770217329208,"version":"3.49.0"},"reference-count":19,"publisher":"Wiley","issue":"3","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":2441,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2003,5]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Reasons for performing study<\/jats:italic>\n                    : Parathyroid hormone (PTH) plays a critical role in the regulation of mineral metabolism in mammals. Until recently, the standard method for PTH measurement has been the 2nd generation intact\u2010PTH (I\u2010PTH) assay. Current evidence indicates that the I\u2010PTH assay binds to the PTH molecule and to an inactive N\u2010terminally truncated PTH fragment that tends to accumulate in the blood of uraemic patients. Therefore, a new 3rd generation PTH assay that detects only the whole PTH molecule (W\u2010PTH; cyclase\u2010activating PTH [CAP]) has been developed.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Objectives<\/jats:italic>\n                    : To validate this more specific W\u2010PTH assay for measurement of equine PTH and evaluate its clinical utility.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    : W\u2010PTH and I\u2010PTH were measured in plasma samples from normal horses (adults and foals) and horses with nutritional secondary hyperparathyroidism (N2HPT) and with chronic renal failure (CRF). Replicate measurements and dilutional paralellism were used for assay validation. Changes in blood ionized calcium were induced by EDTA and CaCl\n                    <jats:sub>2<\/jats:sub>\n                    administration.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    : Performance of the W\u2010PTH assay (accuracy, sensitivity, specificity and ability to detect changes in PTH in response to changes in calcium) was similar to that of the I\u2010PTH assay. Surprisingly, the relative W\u2010PTH concentration in normal horses and foals was higher than the relative I\u2010PTH concentration. W\u2010PTH values remained higher than I\u2010PTH during acute hypo\u2010 and hypercalcaemia. An increase in both W\u2010PTH and I\u2010PTH concentrations was found in horses with N2HPT. In horses with CRF, W\u2010PTH and I\u2010PTH values were very low and no increase in I\u2010PTH was observed.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    : The W\u2010PTH assay can be used for measurement of equine PTH.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Potential relevance<\/jats:italic>\n                    : The use of W\u2010PTH assay is likely to improve the diagnosis of mineral metabolism in horses.\n                  <\/jats:p>","DOI":"10.2746\/042516403776148246","type":"journal-article","created":{"date-parts":[[2007,4,12]],"date-time":"2007-04-12T19:03:27Z","timestamp":1176404607000},"page":"291-295","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":17,"title":["Validation and clinical utility of a novel immunoradiometric assay exclusively for biologically active whole parathyroid hormone in the horse"],"prefix":"10.1111","volume":"35","author":[{"given":"J. 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