{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T07:55:52Z","timestamp":1769154952814,"version":"3.49.0"},"reference-count":33,"publisher":"Wiley","issue":"5","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":1222,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2006,9]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Reasons for performing study<\/jats:italic>\n                    : Sialic acids modulate the metabolite transport across membranes and may be involved in protection against pathogenic agents. The presence of sialoderivatives in the equine mandibular gland requires further study.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Objective<\/jats:italic>\n                    : To biochemically visualise\n                    <jats:italic>in situ<\/jats:italic>\n                    the presence of sialoderivatives, by means of mild and strong periodate oxidation and alcoholic saponification, combined with lectin histochemistry and sialidase digestion in order to hypothesise roles for detached sialoderivatives.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    : Mandibular glands were removed from 8 mature horses of both sexes and subjected to histochemical procedures, including periodate oxidation, saponfication and lectin staining. Controls were based upon the omission of peroxidase\u2010conjugated lectins and respective enzyme\u2010free buffers.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    : The reactivities of PNA and RCA I lectins were affected by sialidase treatment, whether preceded by saponification or not, showing that the dimer N\u2010acetyl\u2010sialic acid\u2010\u03b2\u2010Gal was linked (1\u20133)GalNAc and (1\u20134)GlcNAc. In acinar cells the sequence sialic acid\u2010\u03b2\u2010Gal(1\u20133)GalNAc showed sialic residues acetylated at C\n                    <jats:sub>4<\/jats:sub>\n                    only and at C\n                    <jats:sub>4<\/jats:sub>\n                    and C\n                    <jats:sub>7<\/jats:sub>\n                    and\/or C\n                    <jats:sub>8<\/jats:sub>\n                    and\/or C\n                    <jats:sub>9<\/jats:sub>\n                    (\u03b12\u20136Gal) in both sexes, while in female mandibular gland also C\n                    <jats:sub>4<\/jats:sub>\n                    and C\n                    <jats:sub>9<\/jats:sub>\n                    (\u03b12\u20133Gal) acetylated residues were present. Sialic acid linked to \u03b2\u2010Gal(1\u20134)GlcNAc was prevalently C\n                    <jats:sub>4<\/jats:sub>\n                    and C\n                    <jats:sub>7<\/jats:sub>\n                    and\/or C\n                    <jats:sub>8<\/jats:sub>\n                    and\/or C\n                    <jats:sub>9<\/jats:sub>\n                    (\u03b12\u20136Gal and \u03b12\u20133Gal) acetylated, whereas only a minor quantity showed acetyl groups at C\n                    <jats:sub>7<\/jats:sub>\n                    and\/or C\n                    <jats:sub>8<\/jats:sub>\n                    and\/or C\n                    <jats:sub>9<\/jats:sub>\n                    (\u03b12\u20136Gal) in the acinar cells of both sexes.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Conclusions<\/jats:italic>\n                    : The great variety of sialic acid residues expressed by equine mandibular gland could assume an important role in the defensive mechanisms towards pathogen agents and, compared with those of cattle, probably represents an example of molecular species\u2010specificity related to different alimentary habits.\n                  <\/jats:p>","DOI":"10.2746\/042516406778400637","type":"journal-article","created":{"date-parts":[[2007,3,14]],"date-time":"2007-03-14T19:05:16Z","timestamp":1173899116000},"page":"410-415","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":12,"title":["Equine mandibular gland:\n                    <i>in situ<\/i>\n                    characterisation of sialoderivatives"],"prefix":"10.1111","volume":"38","author":[{"given":"P.","family":"SCOCCO","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"PEDINI","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,5]]},"reference":[{"key":"e_1_2_1_2_1","first-page":"723","article-title":"Variety of sialic acids occurring in the bovine sublingual gland","volume":"9","author":"Accili D.","year":"1994","journal-title":"Histol. Histopath."},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.3891\/acta.chem.scand.14-1809"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1177\/14.4.291"},{"key":"e_1_2_1_5_1","doi-asserted-by":"crossref","first-page":"9842","DOI":"10.1016\/S0021-9258(18)81735-5","article-title":"4\u2010O\u2010acetyl\u2010N\u2010acetylneuraminic acid in the N\u2010linked carbohydrate structures of equine and guinea pig \u03b12\u2010macroglobulins, potent inhibitors of influenza virus infection","volume":"264","author":"Hanaoka K.","year":"1989","journal-title":"J. biol. Chem."},{"key":"e_1_2_1_6_1","first-page":"81","volume-title":"Atti V Conv. Allevamenti di Selvaggina a Scopo Alimentare","author":"Hofmann R.R.","year":"1984"},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)87002-0"},{"key":"e_1_2_1_8_1","doi-asserted-by":"publisher","DOI":"10.1007\/s10719-006-5442-z"},{"key":"e_1_2_1_9_1","doi-asserted-by":"publisher","DOI":"10.1177\/32.9.6379041"},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9861(87)90366-3"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-1281(84)80069-0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/0003-9969(93)90003-5"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00508309"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00270386"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2318(08)60021-9"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/5.8.733"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1042\/bj2800341"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1177\/26.3.632555"},{"key":"e_1_2_1_19_1","first-page":"263","volume-title":"Sialic Acids. Chemistry, Metabolism and Function. Cell Biol. Monographs","author":"Reutter W.","year":"1982"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/0042-6822(83)90507-X"},{"key":"e_1_2_1_21_1","first-page":"65","volume-title":"Methods in Enzymology","author":"Schauer R.","year":"1978"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0065-2318(08)60109-2"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01002741"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF01003432"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1007\/BF00217558"},{"key":"e_1_2_1_26_1","first-page":"47","article-title":"Lectin histochemistry and identification of O\u2010acetylated sialoderivatives in the horse sublingual gland","volume":"43","author":"Scocco P.","year":"1999","journal-title":"Eur. J. Histochem."},{"key":"e_1_2_1_27_1","first-page":"43","article-title":"From conventional to modern histochemistry: a way for the fine characterization of secretion glycoconjugates","volume":"107","author":"Scocco P.","year":"2002","journal-title":"It. J. Anat. Embryol."},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.1038\/440435a"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1177\/10454411900010040201"},{"key":"e_1_2_1_30_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.ph.57.030195.002555"},{"key":"e_1_2_1_31_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1600-0714.1982.tb00138.x"},{"key":"e_1_2_1_32_1","doi-asserted-by":"publisher","DOI":"10.1093\/glycob\/cwg032"},{"key":"e_1_2_1_33_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0008-6215(00)90358-6"},{"key":"e_1_2_1_34_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.1432-1033.1992.tb16626.x"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2746%2F042516406778400637","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.2746\/042516406778400637","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T09:59:05Z","timestamp":1762077545000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.2746\/042516406778400637"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2006,9]]},"references-count":33,"journal-issue":{"issue":"5","published-print":{"date-parts":[[2006,9]]}},"alternative-id":["10.2746\/042516406778400637"],"URL":"https:\/\/doi.org\/10.2746\/042516406778400637","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2006,9]]},"assertion":[{"value":"2010-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}