{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,7]],"date-time":"2026-03-07T14:26:38Z","timestamp":1772893598453,"version":"3.50.1"},"reference-count":28,"publisher":"Wiley","issue":"1","license":[{"start":{"date-parts":[[2010,1,5]],"date-time":"2010-01-05T00:00:00Z","timestamp":1262649600000},"content-version":"vor","delay-in-days":369,"URL":"http:\/\/onlinelibrary.wiley.com\/termsAndConditions#vor"}],"content-domain":{"domain":["beva.onlinelibrary.wiley.com"],"crossmark-restriction":true},"short-container-title":["Equine Veterinary Journal"],"published-print":{"date-parts":[[2009,1]]},"abstract":"<jats:title>Summary<\/jats:title>\n                  <jats:p>\n                    <jats:italic>Reasons for performing study<\/jats:italic>\n                    : Airway inflammation in recurrent airway obstruction (RAO) is triggered by housing affected horses in stables. It has been suggested that RAO is an allergic condition, but innate immune mechanisms are also involved. Fungal products activate innate immune mechanisms through toll\u2010like receptor 2 (TLR2). In human airway epithelium, TLR2 activation leads to interleukin (IL)\u20108 production. This pathway is negatively regulated by the zinc finger protein A20. This study was performed to enhance understanding of innate immune mechanisms in RAO.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Hypothesis<\/jats:italic>\n                    : TLR2 and IL\u20108 mRNA are elevated in RAO during stabling compared with controls. A20 mRNA is negatively associated with the numbers of airway inflammatory cells.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Objectives<\/jats:italic>\n                    : To determine TLR\n                    <jats:bold>2<\/jats:bold>\n                    , IL\u20108 and A20 mRNA expression in lungs of stabled and pastured RAO\u2010affected and control horses.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Methods<\/jats:italic>\n                    : Airway obstruction and inflammatory cell counts in bronchoalveolar lavage were measured, and TLR\n                    <jats:bold>2<\/jats:bold>\n                    , IL\u20108 and A20 mRNA expression quantified by qRT\u2010PCR in 6 RAO\u2010affected and 6 control horses, during and after exposure to hay and straw.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Results<\/jats:italic>\n                    : Airway obstruction and neutrophils were increased in RAO\u2010affected horses during stabling. While stabling increased IL\u2010\n                    <jats:bold>8<\/jats:bold>\n                    , TLR2 and A20 mRNA were unaffected. TLR2 and A20 were significantly correlated (r = 0.83) and A20 mRNA was negatively associated with inflammatory cells.\n                  <\/jats:p>\n                  <jats:p>\n                    <jats:italic>Potential relevance<\/jats:italic>\n                    : Stabling does not lead to an increase in TLR2 expression. Other molecules or processes in the TLR2 cascade might be important in fungal\u2010induced airway inflammation. Equine epithelial\u2010derived A20 may be involved in modulation of airway inflammation.\n                  <\/jats:p>","DOI":"10.2746\/042516408x347592","type":"journal-article","created":{"date-parts":[[2008,12,24]],"date-time":"2008-12-24T19:11:29Z","timestamp":1230145889000},"page":"76-81","update-policy":"https:\/\/doi.org\/10.1002\/crossmark_policy","source":"Crossref","is-referenced-by-count":5,"title":["Expression of toll\u2010like receptor 2 mRNA in bronchial epithelial cells is not induced in RAO\u2010affected horses"],"prefix":"10.1111","volume":"41","author":[{"given":"A.","family":"Berndt","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"F. J.","family":"Derksen","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"P. J.","family":"Venta","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"W.","family":"Karmaus","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"S.","family":"Ewart","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"V.","family":"Yuzbasiyan\u2010Gurkan","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"N. E.","family":"Robinson","sequence":"additional","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","published-online":{"date-parts":[[2010,1,5]]},"reference":[{"key":"e_1_2_1_2_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(03)00142-9"},{"key":"e_1_2_1_3_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00323.2003"},{"key":"e_1_2_1_4_1","doi-asserted-by":"publisher","DOI":"10.1152\/ajplung.00394.2006"},{"key":"e_1_2_1_5_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.vetimm.2003.08.013"},{"key":"e_1_2_1_6_1","doi-asserted-by":"crossref","first-page":"933","DOI":"10.2460\/ajvr.1988.49.06.933","article-title":"Aerosolized Micropolyspora faeni antigen as a cause of pulmonary dysfunction in ponies with recurrent airway obstruction (heaves)","volume":"49","author":"Derksen F.J.","year":"1988","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_7_1","doi-asserted-by":"publisher","DOI":"10.1152\/jappl.1987.62.4.1398"},{"key":"e_1_2_1_8_1","first-page":"1066","article-title":"Bronchoalveolar lavage in ponies with recurrent airway obstruction (heaves)","volume":"132","author":"Derksen F.J.","year":"1985","journal-title":"Am. Rev. respir. Dis."},{"key":"e_1_2_1_9_1","doi-asserted-by":"crossref","first-page":"203","DOI":"10.2460\/ajvr.1992.53.02.203","article-title":"Intradermal testing of horses with chronic obstructive pulmonary disease and recurrent urticaria","volume":"53","author":"Evans A.G.","year":"1992","journal-title":"Am. J. vet. Res."},{"key":"e_1_2_1_10_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI18696"},{"key":"e_1_2_1_11_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(98)00178-0"},{"key":"e_1_2_1_12_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0165-2427(01)00420-2"},{"key":"e_1_2_1_13_1","doi-asserted-by":"publisher","DOI":"10.1165\/rcmb.2003-0438OC"},{"key":"e_1_2_1_14_1","doi-asserted-by":"publisher","DOI":"10.1146\/annurev.cellbio.21.122303.120653"},{"key":"e_1_2_1_15_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(19)37138-8"},{"key":"e_1_2_1_16_1","doi-asserted-by":"publisher","DOI":"10.1074\/jbc.M201683200"},{"key":"e_1_2_1_17_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1993.tb02962.x"},{"key":"e_1_2_1_18_1","doi-asserted-by":"publisher","DOI":"10.1111\/j.2042-3306.1993.tb02960.x"},{"key":"e_1_2_1_19_1","doi-asserted-by":"publisher","DOI":"10.1007\/s11046-005-2669-3"},{"key":"e_1_2_1_20_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.ejphar.2006.03.073"},{"key":"e_1_2_1_21_1","doi-asserted-by":"publisher","DOI":"10.1016\/S0021-9258(18)77165-2"},{"key":"e_1_2_1_22_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516402776249236"},{"key":"e_1_2_1_23_1","doi-asserted-by":"publisher","DOI":"10.1046\/j.1365-2222.2003.01640.x"},{"key":"e_1_2_1_24_1","doi-asserted-by":"publisher","DOI":"10.2746\/042516400777591147"},{"key":"e_1_2_1_25_1","doi-asserted-by":"publisher","DOI":"10.1128\/mr.59.3.423-450.1995"},{"key":"e_1_2_1_26_1","doi-asserted-by":"publisher","DOI":"10.2460\/ajvr.1998.59.08.1039"},{"key":"e_1_2_1_27_1","doi-asserted-by":"publisher","DOI":"10.1172\/JCI200420773"},{"key":"e_1_2_1_28_1","doi-asserted-by":"publisher","DOI":"10.4049\/jimmunol.178.2.970"},{"key":"e_1_2_1_29_1","doi-asserted-by":"publisher","DOI":"10.1016\/j.imbio.2008.02.005"}],"container-title":["Equine Veterinary Journal"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2746%2F042516408X347592","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/onlinelibrary.wiley.com\/doi\/pdf\/10.2746\/042516408X347592","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/pdf\/10.2746\/042516408X347592","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,2]],"date-time":"2025-11-02T13:06:17Z","timestamp":1762088777000},"score":1,"resource":{"primary":{"URL":"https:\/\/beva.onlinelibrary.wiley.com\/doi\/10.2746\/042516408X347592"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2009,1]]},"references-count":28,"journal-issue":{"issue":"1","published-print":{"date-parts":[[2009,1]]}},"alternative-id":["10.2746\/042516408X347592"],"URL":"https:\/\/doi.org\/10.2746\/042516408x347592","archive":["Portico"],"relation":{},"ISSN":["0425-1644","2042-3306"],"issn-type":[{"value":"0425-1644","type":"print"},{"value":"2042-3306","type":"electronic"}],"subject":[],"published":{"date-parts":[[2009,1]]},"assertion":[{"value":"2010-01-05","order":3,"name":"published","label":"Published","group":{"name":"publication_history","label":"Publication History"}}]}}