{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,16]],"date-time":"2026-01-16T02:42:26Z","timestamp":1768531346031,"version":"3.49.0"},"reference-count":17,"publisher":"European Centre for Disease Control and Prevention (ECDC)","issue":"31","license":[{"start":{"date-parts":[[2017,8,3]],"date-time":"2017-08-03T00:00:00Z","timestamp":1501718400000},"content-version":"unspecified","delay-in-days":0,"URL":"http:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2017,8,3]]},"abstract":"<jats:p>Colistin resistance genes <jats:italic>mcr-3<\/jats:italic> and <jats:italic>mcr-1<\/jats:italic> have been detected in an <jats:italic>Escherichia coli<\/jats:italic> isolate from cattle faeces in a Spanish slaughterhouse in 2015. The sequences of both genes hybridised to same plasmid band of ca\u2009250 kb, although colistin resistance was non-mobilisable. The isolate was producing extended-spectrum beta-lactamases and belonged to serotype O9:H10 and sequence type ST533. Here we report an <jats:italic>mcr<\/jats:italic>-3 gene detected in Europe following earlier reports from Asia and the United States.<\/jats:p>","DOI":"10.2807\/1560-7917.es.2017.22.31.30586","type":"journal-article","created":{"date-parts":[[2017,8,11]],"date-time":"2017-08-11T08:38:29Z","timestamp":1502440709000},"update-policy":"https:\/\/doi.org\/10.2807\/1560-7917.es.crp2020","source":"Crossref","is-referenced-by-count":89,"title":["Co-occurrence of colistin-resistance genes mcr-1 and mcr-3 among multidrug-resistant Escherichia coli isolated from cattle, Spain, September 2015"],"prefix":"10.2807","volume":"22","author":[{"given":"Marta","family":"Hern\u00e1ndez","sequence":"first","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, Valladolid, Spain"},{"name":"Departamento de Ingenier\u00eda Agr\u00edcola y Forestal, Tecnolog\u00eda de los Alimentos, Escuela T\u00e9cnica Superior de Ingenier\u00edas Agrarias, Universidad de Valladolid, Palencia, Spain"},{"name":"These authors contributed equally to the manuscript"}]},{"given":"M Roc\u00edo","family":"Iglesias","sequence":"additional","affiliation":[{"name":"These authors contributed equally to the manuscript"},{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"}]},{"given":"David","family":"Rodr\u00edguez-L\u00e1zaro","sequence":"additional","affiliation":[{"name":"These authors contributed equally to the manuscript"},{"name":"\u00c1rea de Microbiolog\u00eda, Departamento de Biotecnolog\u00eda y Ciencia de los Alimentos, Universidad de Burgos, Burgos, Spain"}]},{"given":"Alejandro","family":"Gallardo","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"}]},{"given":"Narciso","family":"Quijada","sequence":"additional","affiliation":[{"name":"Laboratorio de Biolog\u00eda Molecular y Microbiolog\u00eda, Instituto Tecnol\u00f3gico Agrario de Castilla y Le\u00f3n, Valladolid, Spain"}]},{"given":"Pedro","family":"Miguela-Villoldo","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain"}]},{"given":"Maria Jorge","family":"Campos","sequence":"additional","affiliation":[{"name":"MARE - Marine and Environmental Sciences Centre, Instituto Polit\u00e9cnico de Leiria, Peniche, Portugal"}]},{"given":"Segundo","family":"P\u00edriz","sequence":"additional","affiliation":[{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"}]},{"given":"Gema","family":"L\u00f3pez-Orozco","sequence":"additional","affiliation":[{"name":"Subdirecci\u00f3n General de Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura y Pesca, Alimentaci\u00f3n y Medio Ambiente, Madrid, Spain"}]},{"given":"Cristina","family":"de Frutos","sequence":"additional","affiliation":[{"name":"Laboratorio Central de Veterinaria, Ministerio de Agricultura y Pesca, Alimentaci\u00f3n y Medio Ambiente, Algete, Spain"}]},{"given":"Jos\u00e9 Luis","family":"S\u00e1ez","sequence":"additional","affiliation":[{"name":"Subdirecci\u00f3n General de Sanidad e Higiene Animal y Trazabilidad, Ministerio de Agricultura y Pesca, Alimentaci\u00f3n y Medio Ambiente, Madrid, Spain"}]},{"given":"Mar\u00eda","family":"Ugarte-Ruiz","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain"}]},{"given":"Lucas","family":"Dom\u00ednguez","sequence":"additional","affiliation":[{"name":"VISAVET Health Surveillance Centre, Universidad Complutense, Madrid, Spain"},{"name":"Departamento de Sanidad Animal, Facultad de Veterinaria, Universidad Complutense, Madrid, Spain."}]},{"given":"Alberto","family":"Quesada","sequence":"additional","affiliation":[{"name":"Departamento de Bioqu\u00edmica, Biolog\u00eda Molecular y Gen\u00e9tica, Facultad de Veterinaria, Universidad de Extremadura, C\u00e1ceres, Spain"},{"name":"INBIO G+C, Universidad de Extremadura, C\u00e1ceres, Spain"}]}],"member":"7995","reference":[{"issue":"3","key":"r1","doi-asserted-by":"crossref","first-page":"e00543-17","DOI":"10.1128\/mBio.00543-17","article-title":"Novel plasmid-mediated colistin resistance gene mcr-3 in Escherichia coli.","volume":"8","author":"Yin","year":"2017","journal-title":"MBio"},{"key":"r2","unstructured":"European Commission. 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