{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T16:00:24Z","timestamp":1776355224109,"version":"3.51.2"},"reference-count":6,"publisher":"Wiley","issue":"7","license":[{"start":{"date-parts":[[2015,9,1]],"date-time":"2015-09-01T00:00:00Z","timestamp":1441065600000},"content-version":"tdm","delay-in-days":1888,"URL":"http:\/\/doi.wiley.com\/10.1002\/tdm_license_1"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EFS2","EFSA Journal"],"published-print":{"date-parts":[[2010,7]]},"abstract":"<jats:p>Abstract Cylactin\u00ae is a product consisting of viable cells of Enterococcus faecium. The product strain was found to beresistant to kanamycin. A detailed study of the nature of kanamycin resistance demonstrated the absence ofknown acquired genes coding for aminoglycosides\u2010modifying enzymes. Two genetic determinants, theaminoglycoside acetyltransferase gene aac(6\u2019)\u2010li and the gene sfkmr, both intrinsic to E. faecium, were shown tocontribute to the resistance to kanamycin. In the view of the FEEDAP Panel, the resistance of this strain of E.faecium is most likely to be caused by an unknown mechanism that potentiates the effect of the sfkmr gene, andnot by acquisition of resistance genes, and thus is not a cause for concern. When used at 100 times therecommended level, Cylactin\u00ae did not show any adverse effect on performance or mortality of chickens forfattening. Therefore, the additive is safe for the target animals at the proposed conditions of use. E. faeciumNCIMB 10415 is free of known enterococcal virulence factors. No other concerns for consumer safety wereidentified. Cylactin\u00ae is not a skin\/mucosal irritant or skin sensitiser. As the product is formulated with a largeparticle size and the dusting potential is low, inhalation exposure of users will be minimal. Cylactin\u00ae isefficacious in chickens for fattening as demonstrated in three trials showing an increase in final body weightwhen diets were supplemented at or close to the minimum recommended dose (0.3 \u00d7 109 CFU\/kg feed). Cylactin\u00ae is compatible with decoquinate, monensin, robenidine, diclazuril and semduramycin.<\/jats:p>","DOI":"10.2903\/j.efsa.2010.1661","type":"journal-article","created":{"date-parts":[[2016,5,23]],"date-time":"2016-05-23T18:47:13Z","timestamp":1464029233000},"source":"Crossref","is-referenced-by-count":11,"title":["Scientific Opinion on the safety and efficacy of the product Cylactin\u00ae (Enterococcus faecium) as a feed additive for chickens for fattening"],"prefix":"10.2903","volume":"8","author":[{"name":"EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)","sequence":"first","affiliation":[],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"311","reference":[{"key":"10.2903\/j.efsa.2010.1661_ref1","unstructured":"EC (European Commission) , 1997, updated 2003. Opinion on the use of certain micro\u2010organisms as additives in feedingstuffs."},{"key":"10.2903\/j.efsa.2010.1661_ref2","first-page":"1","article-title":"Opinion of the Scientific Panel on Additives and Products or Substances used in Animal Feed on a request from the Commission on the safety of the product Cylactin for dogs and cats","volume":"138","author":"EFSA (European Food Safety Authority)","year":"2004","journal-title":"The EFSA Journal"},{"key":"10.2903\/j.efsa.2010.1661_ref3","first-page":"1","article-title":"Opinion of the Scientific Panel on additives and products or substances used in animal feed (FFEDAP) on the updating of the criteria used in the assessment of the bacteria for resistance to antibiotics of human or veterinary importance","volume":"223","author":"EFSA (European Food Safety Authority)","year":"2005","journal-title":"The EFSA Journal"},{"key":"10.2903\/j.efsa.2010.1661_ref4","first-page":"1","article-title":"Technical guidance. Update of the criteria used in the assessment of bacterial resistance to antibiotics of human or veterinary importance. Prepared by the Panel on Additives and Products or Substances used in Animal Feed","volume":"732","author":"EFSA (European Food safety Authority)","year":"2008","journal-title":"The EFSA Journal"},{"key":"10.2903\/j.efsa.2010.1661_ref5","first-page":"1","article-title":"Technical guidance. Compatibility of zootechnical microbial additives with other additives showing antimicrobial activity. Prepared by the Panel on Additives and Products or Substances used ion Animal Feed","volume":"658","author":"EFSA (European Food Safety Authority)","year":"2008","journal-title":"The EFSA Journal"},{"key":"10.2903\/j.efsa.2010.1661_ref6","volume-title":"Retail Meat Annual Report 2007","author":"NARMS (National Antimicrobial Resistance Monitoring System)","year":"2007"}],"container-title":["EFSA Journal"],"original-title":[],"link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2903%2Fj.efsa.2010.1661","content-type":"unspecified","content-version":"vor","intended-application":"text-mining"},{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2903\/j.efsa.2010.1661","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.2903\/j.efsa.2010.1661\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,4,16]],"date-time":"2026-04-16T15:05:06Z","timestamp":1776351906000},"score":1,"resource":{"primary":{"URL":"http:\/\/doi.wiley.com\/10.2903\/j.efsa.2010.1661"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2010,7]]},"references-count":6,"journal-issue":{"issue":"7"},"URL":"https:\/\/doi.org\/10.2903\/j.efsa.2010.1661","relation":{},"ISSN":["1831-4732","1831-4732"],"issn-type":[{"value":"1831-4732","type":"print"},{"value":"1831-4732","type":"electronic"}],"subject":[],"published":{"date-parts":[[2010,7]]}}}