{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,29]],"date-time":"2026-09-29T16:44:17Z","timestamp":1790700257735,"version":"4.1.0"},"reference-count":14,"publisher":"Wiley","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EFS2","EFSA Journal"],"published-print":{"date-parts":[[2025,12]]},"abstract":"<jats:p>Abstract Parasites in seafood are a recognised food safety concern worldwide. Anisakiasis is a human disease caused by ingestion of larvae of parasitic nematodes of the genus Anisakis, typically present in raw or undercooked seafood. It is considered a significant emerging foodborne disease and was recently ranked among the top 10 of 24 parasitic foodborne infections in Europe, with a rapidly increasing potential to cause illness. Many clinical cases are often misdiagnosed as other gastrointestinal disorders, leading to misinterpretation of data and likely a substantial underestimation of their true prevalence. The issue is further complicated by a rising number of asymptomatic individuals who develop sensitivity to thermostable Anisakis antigens. These otherwise healthy individuals often show elevated anti\u2010Anisakis IgE antibody levels and may have recovered from a mild or undiagnosed gastric anisakiasis without showing allergic symptoms. Because these cases lack obvious clinical signs, this \u2018silent\u2019 form of anisakiasis could affect more than 12% of certain populations and is typically identified only through sero\u2010epidemiological research. Earlier detection approaches, including DNA\u2010based methods such as real\u2010time PCR, demonstrated the potential to identify Anisakis as an allergy\u2010causing agens in marketed seafood. However, these methods had not been validated, and the results, therefore, required cautious interpretation. Here, we present the first validated real\u2010time PCR method designed to improve the detection of Anisakis as an allergy\u2010causing agens and to support more accurate monitoring within the EU seafood market. Additional work to express two known allergic proteins (Ani s 1 and Ani s 7) for immunological testing of hypersensitised patients can open venues for a future complete risk assessment of anisakiasis.<\/jats:p>","DOI":"10.2903\/j.efsa.2025.e231106","type":"journal-article","created":{"date-parts":[[2025,12,15]],"date-time":"2025-12-15T13:14:14Z","timestamp":1765804454000},"source":"Crossref","is-referenced-by-count":1,"title":["Assessing allergenic potential, exposure and risk of Anisakis spp."],"prefix":"10.2903","volume":"23","author":[{"given":"J.","family":"Majstorovi\u0107","sequence":"first","affiliation":[{"name":"Laboratory of Functional Helminthology, Institute of Parasitology, Biology Centre Czech Academy of Sciences Czechia"},{"name":"Federal German Federal Institute for Risk Assessment (BfR) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"U. M.","family":"Herfurth","sequence":"additional","affiliation":[{"name":"Federal German Federal Institute for Risk Assessment (BfR) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"I.","family":"Mladineo","sequence":"additional","affiliation":[{"name":"Laboratory of Functional Helminthology, Institute of Parasitology, Biology Centre Czech Academy of Sciences Czechia"},{"name":"University of Tasmania Australia"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"M.","family":"Winkel","sequence":"additional","affiliation":[{"name":"Federal German Federal Institute for Risk Assessment (BfR) Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"issue":"2","key":"10.2903\/j.efsa.2025.e231106_ref1","first-page":"652","article-title":"Parasites of fish and risks to public health","volume":"16","author":"Adams","year":"1997","journal-title":"Revue Scientifique et Technique (International Office of 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