{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T08:04:13Z","timestamp":1780041853687,"version":"3.53.1"},"reference-count":23,"publisher":"Wiley","issue":"5","content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":["EFS2","EFSA Journal"],"published-print":{"date-parts":[[2026,5]]},"abstract":"<jats:p>Abstract Following a request from the European Commission, the Panel on Additives and Products or Substances used in Animal Feed (FEEDAP) of EFSA was asked to deliver a scientific opinion on the safety and efficacy of riboflavin 80% (vitamin B2) produced by Saccharomyces cerevisiae ATCC SD\u20108628 as a nutritional feed additive for all animal species. The additive is produced by a genetically modified production strain. The production strain harbours genes of concern coding for resistance to antimicrobials. However, viable cells and DNA of the production strain were not detected in the final product. Therefore, the use of S. cerevisiae ATCC SD\u20108628 to produce vitamin B2 does not raise safety concerns. The use of riboflavin 80% produced by S. cerevisiae ATCC SD\u20108628 in animal nutrition does not represent a safety concern for the target species, consumers and for the environment. Based on the information present in the safety data sheet, the additive should be considered an irritant to skin, eyes and respiratory tract. Exposure by any route is considered a risk. The additive under assessment is effective in covering the animals' requirements of vitamin B2 when administered via feed.<\/jats:p>","DOI":"10.2903\/j.efsa.2026.10120","type":"journal-article","created":{"date-parts":[[2026,5,26]],"date-time":"2026-05-26T09:15:16Z","timestamp":1779786916000},"source":"Crossref","is-referenced-by-count":0,"title":["Safety and efficacy of a feed additive consisting of riboflavin 80% (vitamin B2) produced with Saccharomyces cerevisiae ATCC SD\u20108628 for all animal species (Amyris Inc)"],"prefix":"10.2903","volume":"24","author":[{"name":"EFSA Panel on Additives and Products or Substances used in Animal Feed (FEEDAP)","sequence":"first","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto Edoardo","family":"Villa","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Azimonti","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Eleftherios","family":"Bonos","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Henrik","family":"Christensen","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mojca","family":"Durjava","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Birgit","family":"Dusemund","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ronette","family":"Gehring","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Boet","family":"Glandorf","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maryline","family":"Kouba","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"L\u00f3pez\u2010Alonso","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca","family":"Marcon","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Martelli","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Carlo","family":"Nebbia","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alena","family":"Pechov\u00e1","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Miguel","family":"Prieto\u2010Maradona","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Katerina","family":"Theodoridou","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrey","family":"Yurkov","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Baltasar","family":"Mayo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rosella","family":"Brozzi","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jaume","family":"Galobart","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Elisa","family":"Pettenati","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maria Vittoria","family":"Vettori","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Fabiola","family":"Pizzo","sequence":"additional","affiliation":[],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"311","reference":[{"key":"10.2903\/j.efsa.2026.10120_ref1","doi-asserted-by":"crossref","unstructured":"EFSA (European Food Safety Authority) . (2021). EFSA statement on the requirements for whole genome sequence analysis of microorganisms intentionally used in the food chain. EFSA Journal, 19(7), 6506. https:\/\/doi.org\/10.2903\/j.efsa.2021.6506","DOI":"10.2903\/j.efsa.2021.6506"},{"key":"10.2903\/j.efsa.2026.10120_ref2","doi-asserted-by":"crossref","unstructured":"EFSA BIOHAZ Panel (EFSA Panel on Biological Hazards) , Koutsoumanis, K., Allende, A., \u00c1lvarez\u2010Ord\u00f3\u00f1ez, A., Bolton, D., Bover\u2010Cid, S., Chemaly, M., De Cesare, A., Hilbert, F., Lindqvist, R., Nauta, M., Peixe, L., Ru, G., Simmons, M., Skandamis, P., Suffredini, E., Cocconcelli, P. S., Fern\u00e1ndez Esc\u00e1mez, P. S., Prieto Maradona, M., \u2026 Herman, L. (2023). Scientific opinion on the update of the list of qualified presumption of safety (QPS) recommended microorganisms intentionally added to food or feed as notified to EFSA. EFSA Journal, 21(1), 7747. https:\/\/doi.org\/10.2903\/j.efsa.2023.7747","DOI":"10.2903\/j.efsa.2023.7747"},{"key":"10.2903\/j.efsa.2026.10120_ref3","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) . (2014). Scientific opinion on the safety and efficacy of vitamin B2 (80%) as riboflavin produced by Bacillus subtilis for all animal species, based on a dossier submitted by VITAC EEIG. EFSA Journal, 12(1), 3531. https:\/\/doi.org\/10.2903\/j.efsa.2014.3531","DOI":"10.2903\/j.efsa.2014.3531"},{"key":"10.2903\/j.efsa.2026.10120_ref4","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) . (2016). Scientific opinion on safety and efficacy of vitamin B2 (riboflavin and riboflavin 5\u2032\u2010phosphate ester monosodium salt) produced by Bacillus subtilis (DSM 17339 and DSM 23984) for all animal species based on a dossier submitted by DSM. EFSA Journal, 14(1), 4349. https:\/\/doi.org\/10.2903\/j.efsa.2016.4349","DOI":"10.2903\/j.efsa.2016.4349"},{"key":"10.2903\/j.efsa.2026.10120_ref5","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. L., Bories, G., Chesson, A., Cocconcelli, P. S., Flachowsky, G., Gropp, J., Kolar, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Mantovani, A., Mayo, B., Ramos, F., Saarela, M., \u2026 Innocenti, M. L. (2017a). Guidance on the assessment of the safety of feed additives for the consumer. EFSA Journal, 15(10), 5022. https:\/\/doi.org\/10.2903\/j.efsa.2017.5022","DOI":"10.2903\/j.efsa.2017.5022"},{"key":"10.2903\/j.efsa.2026.10120_ref6","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. L., Bories, G., Chesson, A., Cocconcelli, P. S., Flachowsky, G., Gropp, J., Kolar, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Mantovani, A., Mayo, B., Ramos, F., Saarela, M., \u2026 Innocenti, M. L. (2017b). Guidance on the identity, characterisation and conditions of use of feed additives. EFSA Journal, 15(10), 5023. https:\/\/doi.org\/10.2903\/j.efsa.2017.5023","DOI":"10.2903\/j.efsa.2017.5023"},{"key":"10.2903\/j.efsa.2026.10120_ref7","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. L., Bories, G., Chesson, A., Cocconcelli, P. S., Flachowsky, G., Gropp, J., Kolar, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Mantovani, A., Mayo, B., Ramos, F., Saarela, M., \u2026 Martino, L. (2017c). Guidance on the assessment of the safety of feed additives for the target species. EFSA Journal, 15(10), 5021. https:\/\/doi.org\/10.2903\/j.efsa.2017.5021","DOI":"10.2903\/j.efsa.2017.5021"},{"key":"10.2903\/j.efsa.2026.10120_ref8","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. L., Bories, G., Chesson, A., Cocconcelli, P. S., Flachowsky, G., Gropp, J., Kolar, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Mantovani, A., Mayo, B., Ramos, F., Saarela, M., \u2026 Galobart, J. (2018b). Guidance on the characterisation of microorganisms used as feed additives or as production organisms. EFSA Journal, 16(3), 5206. https:\/\/doi.org\/10.2903\/j.efsa.2018.5206","DOI":"10.2903\/j.efsa.2018.5206"},{"key":"10.2903\/j.efsa.2026.10120_ref9","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Rychen, G., Aquilina, G., Azimonti, G., Bampidis, V., Bastos, M. L., Bories, G., Chesson, A., Flachowsky, G., Gropp, J., Kolar, B., Kouba, M., Lopez\u2010Alonso, M., Lopez Puente, S., Mantovani, A., Mayo, B., Ramos, F., Saarela, M., Villa, R. E., \u2026 Cocconcelli, P. S. (2018c). Scientific opinion on the safety of vitamin B2 (80%) as riboflavin produced by Bacillus subtilis KCCM\u201010445 for all animal species. EFSA Journal, 16(3), 5223. https:\/\/doi.org\/10.2903\/j.efsa.2018.5223","DOI":"10.2903\/j.efsa.2018.5223"},{"key":"10.2903\/j.efsa.2026.10120_ref10","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Bampidis, V., Bastos, M., Christensen, H., Dusemund, B., Kouba, M., Kos Durjava, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Marcon, F., Mayo, B., Pechov\u00e1, A., Petkova, M., Ramos, F., Sanz, Y., Villa, R. E., Woutersen, R., Brock, T., de Knecht, J., \u2026 Azimonti, G. (2019). Guidance on the assessment of the safety of feed additives for the environment. EFSA Journal, 17(4), 5648. https:\/\/doi.org\/10.2903\/j.efsa.2019.5648","DOI":"10.2903\/j.efsa.2019.5648"},{"key":"10.2903\/j.efsa.2026.10120_ref11","unstructured":"European Medicine Agency (EMA) , & Committee for Medicinal Products for Veterinary Use (CVMP) . (2023). VICH GL18(R2) impurities: Residual solvents in new veterinary medicinal products, active substances and excipients (revision 2) (EMA\/CVMP\/VICH\/502\/1999)."},{"key":"10.2903\/j.efsa.2026.10120_ref12","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Bampidis, V., Azimonti, G., Bastos, M. L., Christensen, H., Durjava, M., Dusemund, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Marcon, F., Mayo, B., Pechov\u00e1, A., Petkova, M., Ramos, F., Villa, R. E., Woutersen, R., Brantom, P., Chesson, A., \u2026 Galobart, J. (2023). Guidance on the assessment of the safety of feed additives for the users. EFSA Journal, 21(12), 8469. https:\/\/doi.org\/10.2903\/j.efsa.2023.8469","DOI":"10.2903\/j.efsa.2023.8469"},{"key":"10.2903\/j.efsa.2026.10120_ref13","doi-asserted-by":"crossref","unstructured":"EFSA FEEDAP Panel (EFSA Panel on Additives and Products or Substances used in Animal Feed) , Bampidis, V., Azimonti, G., Bastos, M. L., Christensen, H., Durjava, M., Dusemund, B., Kouba, M., L\u00f3pez\u2010Alonso, M., L\u00f3pez Puente, S., Marcon, F., Mayo, B., Pechov\u00e1, A., Petkova, M., Ramos, F., Villa, R. E., Woutersen, R., Dierick, N., Gropp, J., \u2026 Ortu\u00f1o, J. (2024). Guidance on the assessment of the efficacy of feed additives. EFSA Journal, 22(7), 8856. https:\/\/doi.org\/10.2903\/j.efsa.2024.8856","DOI":"10.2903\/j.efsa.2024.8856"},{"key":"#cr-split#-10.2903\/j.efsa.2026.10120_ref14.1","unstructured":"EFSA NDA Panel (EFSA Panel on Dietetic Products, Nutrition and Allergies) . (2013). Scientific Opinion on the substantiation of a health claim related to riboflavin (vitamin B2) and contribution to normal energy\u2010yielding metabolism pursuant to Article 14 of Regulation"},{"key":"#cr-split#-10.2903\/j.efsa.2026.10120_ref14.2","doi-asserted-by":"crossref","unstructured":"(EC) No 1924\/2006. EFSA Journal, 11(10), 3410, 10 pp. https:\/\/doi.org\/10.2903\/j.efsa.2013.3410","DOI":"10.2903\/j.efsa.2013.3410"},{"key":"10.2903\/j.efsa.2026.10120_ref15","unstructured":"GfE (Gesellschaft f\u00fcr Ern\u00e4hrungsphysiologie) . (1995). Recommendations for the supply of energy and nutrients to beef cattle. DLG\u2010Verlag, Frankfurt am Main, 85 pp. [in German]."},{"key":"10.2903\/j.efsa.2026.10120_ref16","unstructured":"GfE (Gesellschaft f\u00fcr Ern\u00e4hrungsphysiologie) . (2001). Recommendations for the supply of energy and nutrients to dairy cows and heifers. DLG\u2010Verlag, Frankfurt am Main, 136 pp. [in German]."},{"key":"10.2903\/j.efsa.2026.10120_ref17","unstructured":"GfE (Gesellschaft f\u00fcr Ern\u00e4hrungsphysiologie) . (2003). Recommendations for the supply of energy and nutrients to goats. DLG\u2010Verlag, Frankfurt am Main, 121 pp."},{"issue":"7","key":"10.2903\/j.efsa.2026.10120_ref18","doi-asserted-by":"crossref","first-page":"38","DOI":"10.1093\/nar\/gky014","article-title":"Complete genomic and transcriptional landscape analysis using third\u2010generation sequencing: A case study of Saccharomyces cerevisiae CEN.PK113\u20107D","volume":"46","author":"Jenjaroenpun","year":"2018","journal-title":"Nucleic Acids Research"},{"key":"10.2903\/j.efsa.2026.10120_ref19","doi-asserted-by":"crossref","unstructured":"Nijkamp, J. F., van den Broek, M., Datema, E., de Kok, S., Bosman, L., Luttik, M. A., Daran\u2010Lapujade, P., Vongsangnak, W., Nielsen, J., Heijne, W. H. M., Klaassen, P., Paddon, C. J., Platt, D., K\u00f6tter, P., van Ham, R. C., Reinders, M. J. T., Pronk, J. T., de Ridder, D., & Jean\u2010Marc Daran, J. M. (2012). De novo sequencing, assembly and analysis of the genome of the laboratory strain Saccharomyces cerevisiae CEN.PK113\u20107D, a model for modern industrial biotechnology. Microbial Cell Factories, 11, 36.","DOI":"10.1186\/1475-2859-11-36"},{"key":"10.2903\/j.efsa.2026.10120_ref20","volume-title":"Nutrient requirements of dairy cattle. 8th revised edition","author":"NRC (National Academies of Sciences, Engineering, and Medicine)","year":"2021"},{"key":"10.2903\/j.efsa.2026.10120_ref21","first-page":"232","volume-title":"Nutrient requirements of beef cattle. 7th revised edition","author":"NRC (National Research Council)","year":"1996"},{"key":"10.2903\/j.efsa.2026.10120_ref22","first-page":"362","volume-title":"Nutrient requirements of small ruminants. Sheep, goats, cervids, and new world camelids","author":"NRC (National Research Council)","year":"2007"}],"container-title":["EFSA Journal"],"original-title":[],"link":[{"URL":"https:\/\/api.wiley.com\/onlinelibrary\/tdm\/v1\/articles\/10.2903\/j.efsa.2026.10120","content-type":"application\/pdf","content-version":"vor","intended-application":"text-mining"},{"URL":"http:\/\/onlinelibrary.wiley.com\/wol1\/doi\/10.2903\/j.efsa.2026.10120\/fullpdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,5,29]],"date-time":"2026-05-29T07:31:15Z","timestamp":1780039875000},"score":1,"resource":{"primary":{"URL":"http:\/\/doi.wiley.com\/10.2903\/j.efsa.2026.10120"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026,5]]},"references-count":23,"journal-issue":{"issue":"5"},"URL":"https:\/\/doi.org\/10.2903\/j.efsa.2026.10120","relation":{},"ISSN":["1831-4732","1831-4732"],"issn-type":[{"value":"1831-4732","type":"print"},{"value":"1831-4732","type":"electronic"}],"subject":[],"published":{"date-parts":[[2026,5]]}}}